2023
DOI: 10.1166/sam.2023.4535
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Cytotoxicity Modelling of Pasteurella multocida Toxin and Its Histological Study

Heng Lu,
Huanhuan Shen,
Yong Huang

Abstract: In this study, we investigated the molecular mechanism by which the recombinant multicidal Bartonella toxin rPMT damages PK15 cells. We successfully constructed the prokaryotic expression vector pCold I-toxA and identified suitable expression and purification conditions for rPMT. Using the CCK8 assay, we established a cellular damage model and found that PK15 cells were significantly affected by rPMT infection at a concentration of 20 ug/mL for 24 h. Flow cytometry experiments revealed that rPMT induced apopt… Show more

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