Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells

Prasetyo, Eric Priyo; Widjiastuti, Ira; Cahyani, Febriastuti; Kuntjoro, Mefina; Hendrijantini, Nike; Hariyani, Ninuk; Winoto, Ervina Restiwulan; Nugraha, Alexander Patera; Goenharto, Sianiwati; Susilowati, Helen; Hendrianto, Eryk; Rantam, Fedik Abdul

doi:10.1590/pboci.2020.141

Cited by 12 publications

(8 citation statements)

References 12 publications

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“…3 MSCs have been successfully extracted from various tissues, such as the umbilical cord, hair follicles, and adipose tissue, including orofacial tissues. [4][5][6][7][8] MSCs can be derived from orofacial tissue, including the dental pulp of permanent or deciduous teeth, the periodontal ligament, and the gingiva. [9][10][11][12][13] One of the potential and superior sources of MSCs from the oral cavity was in the gingiva.…”

Section: Introductionmentioning

confidence: 99%

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

et al. 2020

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Objective This study aims to confirm whether the GDMSCs isolated from rabbit’s (Oryctolagus cuniculus) gingiva are mesenchymal stem cells (MSCs). Materials and Methods This study design was partly quasi-experimental with an observational design. GDMSCs were isolated from the gingiva of healthy male rabbits (O. cuniculus) (n = 2), 6 months old, and 3 to 5 kg of body weight. The specific cell surface markers of MSCs; clusters of differentiation (CD), namely, CD44, CD73, CD90, CD105, and CD200 expressions; and hematopoietic stem cell surface markers CD34 and CD45 were examined using flow cytometry and immunohistochemistry with immunofluorescence. The osteogenic differentiation of isolated GDMSCs was examined using alizarin red staining. Results GDMSCs in the fourth passage showed a spindle-like formation and fibroblast-like cells that attached to the base of the culture plate. GDMSCs were MSCs that positively expressed CD44, CD73, CD90, CD105, and CD200 but did not express CD34 and CD45 when examined using flow cytometry and immunohistochemical analysis. GDMSCs had osteogenic differentiation confirmed by calcified deposits in vitro with a red–violet and brownish color after alizarin red staining. Conclusion GDMSCs isolated from the rabbits (O. cuniculus) were confirmed as MSCs in vitro documented using immunohistochemistry and flow cytometry. GDMSCs can differentiate into osteogenic lineage in vitro that may be suitable for regenerative dentistry.

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Section: Introductionmentioning

confidence: 99%

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

et al. 2020

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“…The alkali condition from calcium hydroxide would impact the surrounding tissues, including healing, anti-inflammation 17 and cytotoxic, leading to apoptosis. 18,19 The result of this study showed a significant difference between CH-iodophors and CH-barium sulfate. The ability of CH-iodophors to inhibit E. faecalis and P. gingivalis growths is significantly greater than that of the CH-barium sulfate root canal dressing.…”

Section: Enterococcus Faecalis and Porphyromonas Gingivalismentioning

confidence: 63%

The antibacterial efficacy of calcium hydroxide–iodophors and calcium hydroxide–barium sulfate root canal dressings on Enterococcus faecalis and Porphyromonas gingivalis in vitro

Prasetyo

Juniarti

Sampoerno

et al. 2022

Dent. J. (Majalah Kedokt. Gigi)

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Background: A successful endodontic treatment is inseparable from the right choice of root canal dressing. The right choice of medicaments would result in patient satisfaction. Enterococcus faecalis (E. faecalis) and Porphyromonas gingivalis (P. gingivalis) are usually found in failed root canal treatments. Calcium hydroxide is a gold standard dressing that creates an alkaline environment in the root canal and has a bactericidal effect. Commercially, there are calcium hydroxide dressings with supporting additions, including calcium hydroxide–iodophors (CH–iodophors) and Calcium hydroxide–barium sulfate (CH–barium sulfate). Purpose: This study aimed to compare the antibacterial efficacy between CH–iodophors and CH–barium sulfate root canal dressings on E. faecalis and P. gingivalis. Methods: CH–iodophors and CH–barium sulfate were obtained commercially. E. faecalis and P. gingivalis were obtained from stock culture taken from the root canal of failed endodontic treatment. E. faecalis and P. gingivalis were cultured in Petri dishes, and for each bacterium, 12 wells were made in the media. Six wells were used for the CH–iodophors group, and six wells were used for the CH–barium sulfate group. CH–iodophors and CH–barium sulfate were deployed in the wells in E. faecalis and P. gingivalis cultured media in the Petri dishes. After incubation, the inhibition zone diameters were measured. An independent t-test was used for analysis, and the significance level was set at 5%. Results: There is a significant difference in the antibacterial efficacy of CH–iodophors and that of CH–barium sulfate on E. faecalis and P. gingivalis (p = 0.00001). Conclusion: CH–iodophors have a higher antibacterial efficacy than CH–barium sulfate on both E. faecalis and P. gingivalis.

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“…Pulp-capping treatment with calcium hydroxide is said to be successful if calcium hydroxide can dissociate into ionic forms Ca 2 + and OH -. 15 This is an ionization process in tissues that explains the antibacterial properties of calcium hydroxide. The dissociation of calcium hydroxide into Ca 2 + and OHdepends on the carrier that is used.…”

Section: Discussionmentioning

confidence: 99%

Dosage Effectiveness of the Combination Calcium Hydroxide-Propolis as an Inhibitor for Aggregatibacter actinomycetemcomitans Biofilm

Ira Widjiastuti,

Moch. Mudjiono,

Nancy Cynthia Sudiartha

et al. 2021

Indian Journal of Forensic Medicine & Toxicology

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Objective: Calcium hydroxide is widely used as pulp-capping material, but it is resistant to Aggregatibacter actinomycetemcomitans bacteria. Because of this deficiency, many researchers are considering using alternative natural ingredients, such as propolis. Propolis has been widely used in dentistry due to its properties and because it has active ingredients that can act as antibacterial agents. It is hoped that when calcium hydroxide and propolis are combined, the combination can overcome the disadvantages. The aim of this study was to determine the dosage effectiveness of the combination calcium hydroxide-propolis as an inhibitor for A. actinomycetemcomitans biofilm. Material and methods: The sample was divided into five groups. The positive control group is a combination of calcium hydroxide-sterile aquadest, and the negative control group is A. actinomycetemcomitans bacteria. Group I is a combination of calcium hydroxidepropolis with a ratio of 1:1, group II has a ratio of 1:1.5, and group III has a ratio of 1:2. Each group consists of five replications. Combination of calcium hydroxide-propolis and A. actinomycetemcomitans bacterial suspension was incubated for 2x24 hours then washed three times using PBS. Furthermore, 2% of crystal violet was used for staining and covered with aluminum foil and then incubated for 15 minutes. Isopropanol 200µL was added, followed by a measurement of OD with an ELISA reader, and the OD values were calculated using an inhibition biofilm formula. Results: Group II has the largest mean inhibition with 76% compared to groups one and three. Conclusion: A combination of calcium hydroxide and propolis with a ratio of 1: 1.5 is more effective than 1:1 or 1:2 to inhibit A. actinomycetemcomitans biofilm.

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Cytotoxicity of Calcium Hydroxide on Human Umbilical Cord Mesenchymal Stem Cells

Cited by 12 publications

References 12 publications

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

Gingival-Derived Mesenchymal Stem Cell from Rabbit (Oryctolagus cuniculus): Isolation, Culture, and Characterization

The antibacterial efficacy of calcium hydroxide–iodophors and calcium hydroxide–barium sulfate root canal dressings on Enterococcus faecalis and Porphyromonas gingivalis in vitro

Dosage Effectiveness of the Combination Calcium Hydroxide-Propolis as an Inhibitor for Aggregatibacter actinomycetemcomitans Biofilm

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