Cytotoxin-associated gene A increases carcinogenicity of helicobacter pylori in colorectal adenoma

Abstract

Objective: This study aimed to investigate the correlation of Helicobacter pylori (Hp) infection with disease risk and severity of colorectal adenoma, also to explore the association of cytotoxin-associated gene A (CagA) positive (CagA+)- Hp infection with gastrin and ki-67 expressions in colorectal adenoma patients. Methods: There were 1000 colorectal adenoma patients and 1500 controls consecutively enrolled, then Hp infection status was determined by 14C urea breath test and rapid urease test. Also, serum CagA expression and gastrin expression of colorectal adenoma patients were determined by enzyme-linked immunosorbent assay. Ki-67 expression in adenoma tissue of colorectal adenoma patients was assessed using immunohistochemistry. Results: Hp+ rate in colorectal adenoma patients (623 (62.3%)) was more elevated than that in controls (814 (54.3%)). Multivariate logistic regression model analysis disclosed that Hp+ was an independent risk factor for colorectal adenoma. Additionally, Hp+ was positively associated with tumor size and high-grade intraepithelial neoplasia in colorectal adenoma patients. Also, serum gastrin expression and intratumoral ki-67 expression were higher in Hp+ CagA+ patients and Hp+ CagA− patients compared to Hp− patients, and they were also higher in Hp+ CagA+ patients compared to Hp+ CagA− patients. Conclusion: Hp infection positively associates with higher disease risk and worse disease conditions of colorectal adenoma, and CagA enhances the carcinogenicity of Hp in colorectal adenoma.

Research Progress of Intestinal Microecology in the Pathogenesis of Colorectal Adenoma and Carcinogenesis

“…It is suggested that H. pylori infection is an independent risk factor for colon adenoma, especially in the case of advanced or multiple adenomas. This notion was also demonstrated by the Zhang et al 90 team. The positive rate of H. pylori was higher in patients with CRA than in the control group.…”

Analysis of serum gastrin‐17 and Helicobacter pylori antibody in healthy Chinese population

“…Measurement of H pylori antibody in our study was conducted using a typing test. The type 1 H pylori produces vacuolating cytotoxin (VacA) and cytotoxin‐associated protein (CagA) and is more infectious and pathogenic than the type 2 H pylori which produces urease subunit A/B (UreA/B) and no cytotoxins 20‐22 …”

“…The type 1 H pylori produces vacuolating cytotoxin (VacA) and cytotoxin-associated protein (CagA) and is more infectious and pathogenic than the type 2 H pylori which produces urease subunit A/B (UreA/B) and no cytotoxins. [20][21][22] In the H pylori-infected group, 68.02% were infected with type 1 H pylori, and 31.98% were infected with type 2 H pylori. These data showed that most of the H pylori infection in our region were caused by type 1 H pylori and demonstrated that the type 1 H pylori was more infectious than type 2 H pylori.…”

Regulation effect of miR-7 on intervening colorectal cancer rats with HP infection through Akt/GSK-3β/β-catenin pathway

“…Colorectal cancer is a malignant tumor of the digestive tract that usually occurs in the colon and rectum with high clinical morbidity and fatality rate. As the eating habit of Chinese people has changed in recent years, the incidence of colorectal cancer is on the rise, which seriously affects the physical and mental health and further endangers the life safety of patients (7)(8). HP, as one of the common bacteria for chronic infection, is closely related to several stomach diseases and has a certain correlation with the occurrence of colorectal cancer (9).…”