D.SAP, an apple-based formulation could treat SARS-CoV-2 infection, and reduce associated inflammatory responses in COVID-19 infected mice model

Abstract

Introduction: The SARS-CoV-2 virus is a virus that may cause severe respiratory disorders with an unclear rate of death. Natural vinegar may have an immune-boosting quality that effectively fights influenza-like respiratory infections. We aimed to evaluate the role of a specially formulated Iranian apple vinegar called Dezhakam sap (D.SAP) in the mice model of covid-19 in the treatment of infection and reduction of covid19-associated lung inflammation. Methods: We designed a covid-19-positive model of male inbred BALB/c mice by viral exposure. Then all mice were examined for active SARS-CoV-2 infection. Mice with positive covid-19 infection were separated into different groups to study the effect of treatment by different concentrations of D.SAP smoke for seven days. Expression assessments for viral RNA on covid-19 affected mice models for three SARS-CoV-2 genes (RDRP, N, and E) and three inflammation markers (IL6, IL1b, and TNF) in lung tissue of all mice were assessed using Real-time PCR. Results: Results showed a significant decrease in viral load in the RDRP gene, N gene, and E gene in all infected rats treated with D.SAP compared with off-treatment mice. In addition, all three inflammation factor genes in infected mice that were treated with D.SAP were significantly reduced compared to the off-treatment infected mice. Conclusion: Findings showed the effectiveness of specially formulated apple vinegar D.SAP to attack new coronavirus. In addition, results may suggest the effectiveness of D.SAP to reduce the lethal inflammation in COVID-19 infection with low side effects. Anti-inflammatory agents block certain substances in the body that cause inflammation. D.SAP may consider a potential natural anti-inflammatory that may increase the survival rate of COVID-19 infection. It seems these effects could be related to the antimicrobial and antioxidant effects of D.SAP due to its polyphenolic compounds.

Using D.SAP, an apple-based formulation for treatment of human papilloma virus, a case report

“…Early biochemical and microbial analysis showed that D.SAP is an apple-based formulation, rich in probiotics and antioxidants compounds. There are several mechanisms associated with the immune boosting and anti-in ammation effects of probiotics and antioxidant compounds (12).…”

“…Because of the selection process of apples and the special traditional production of D.SAP, it is antioxidants, probiotic compounds, micronutrients, and cellulose ber rich. The effects of D.SAP contents on the treatment of acute respiratory disorders such as in uenza and SARS-CoV-2 virus regarding its anti-in ammation effects and immune boosting have been reported (12). In this case report we report the usage of DSAP for treatment of a female case with chronic HPV-induced lesions.…”