D1S80 Polymorphism in Istanbul (Turkey)

Abstract

Blood samples were obtained from 78 randomly selected, healthy, unrelated individuals from both sexes and were examined in order to estimate the allele and genotype frequencies of D1S80 locus. DNA was extracted using Chelex 100 extraction method (1). The amplification was carried out according to the instructions of AmpliFLP D1S80 PCR amplification kit (2). The PCR products were analyzed using a vertical denaturating polyacrylamide gel electrophoresis. The gels were silver stained. The statistical analysis was done by the gene count method and it revealed no significant deviation from Hardy-Weinberg expectations. The complete data including the comparison with various Turkish and different populations (3) is available at http://istanbul.edu.tr/enstituler/ forensic/popgen-02.htm.

Allele Frequency of VNTR Locus D1S80 Observed in Denizli Province of Turkey

“…Mahdieh et al (2005) showed that alleles 23 and 18 are most common in the Iranian population. We did not observe allele 23 in this study, but it was observed at a frequency of 0.007 by Akgunes et al (2002) and 0.009 by Ç akir et al (2001). Sachdeva et al (2004) showed higher frequencies of allele 31 in Thoti and Kolam populations (9-14%) than in other Indian populations.…”

“…Some investigations have studied the frequency of D1S80 alleles in the Turkish population; for example, Attila et al (2004) investigated this locus for prenatal diagnosis. Ç akir et al (2001) and Akgunes et al (2002) investigated D1S80 polymorphisms in the Turkish population using agarose and polyacrylamide gel. The most common alleles found were allele 24 and allele 18.…”

“…Ç akir et al (2001, using gel electrophoresis, found that allele 24 (0.46%) was the most common allele in the central Anatolian Turkish population living in Ankara. Akgunes et al (2002), using polyacrylamide gel, reported that the frequency of D1S80 allele 24 was 0.346 in the mixed Turkish population living in Istanbul.…”

“…Since available data were reported at regional bases, they do not represent the whole population (Ç akir et al 2001;Akgunes et al 2002Akgunes et al ). Ç akir et al (2001, using gel electrophoresis, found that allele 24 (0.46%) was the most common allele in the central Anatolian Turkish population living in Ankara.…”