Đặc điểm sinh học của biến chủng Streptomyces orientalis 4912-81- 61 sinh Vancomycin

Abstract

Vacomycin, which is produced by Streptomyces orientalis, is an antibiotic used in clinical treatment against pathogens ressistant to many popular antibacterial agents. The mutant strain Streptomyces orientalis 4912-81-61 producing high yeild of vancomycin was obtained as the result of N-methyl-N-nitro-N nitrosoguanidine treament on the protoplasts of orginal strain S. orientalis 4912. In the present work, the mutant strain Streptomyces orientalis 4912-81-61 was studied with respect to the biological characteristics. Antibiotic production of this mutant strain was enhanced by 94,3% compared to the original strain. The antibiotic produced by this mutant strain was detemined as vancomycin with a anti-microbial spectrum, inhibiting tested strains of general Bacillus, Staphylococcus and Escherichia. This mutant strain grow at favourable conditions such as pH range of 6-8, temperature 28-30oC, in medium containing NaCl concentration not more than 6%. The strain S. orientalis 4912-81-61 express enzyme activites to degrade casein, gelatin and starch. The fermentation process producign cancomycin by the mutant strain S.orientalis 4912-81-61 should be performed more convennient as its abilities of enzyme productivity and utilization of different sugars.