Dactylopius opuntiae: control by the Fusarium incarnatum–equiseti species complex and confirmation of mortality by DNA fingerprinting

Carneiro-Leão, Mariele Porto; Tiago, Patrícia Vieira; Medeiros, Lílian Vieira de; Oliveira, Neiva Tinti de

doi:10.1007/s10340-017-0841-4

Cited by 20 publications

(9 citation statements)

References 26 publications

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“…Isolates of the FIESC have been tested against various species of insects such as Dryocosmus kuriphilus Yasumatsu with reported total mortality of 70% (Addario & Turchetti, 2011), Coccus hesperidum L. with observed total mortality of 70% (Fan et al., 2014), Matsucoccus matsumurae Kuwana with corrected mortality of 83% (Liu et al., 2014), Bemisia tabaci Gennadius with 100% cumulative mortality (Anwar et al., 2017), Nasutitermes corniger Motschulsky with cumulative mortality confirmed of 38–96% (Diniz et al., 2021), and Dactylopius opuntiae Cockerell (Carneiro‐Leão et al., 2017). In the last case, 25 isolates were tested and confirmed mortality against D. opuntiae ranged from 8 to 67%.…”

Section: Discussionmentioning

confidence: 99%

Entomopathogenicity of fungi in combination with Ricinus communis extract for the control of Aleurocanthus woglumi

Barbosa

Santos

Diniz

et al. 2021

Entomologia Exp Applicata

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Citrus is among the most important crops in the world, and has suffered losses of up to 80% due to the citrus blackfly, Aleurocanthus woglumi Ashby (Hemiptera: Aleyrodidae). The combined use of entomopathogenic fungi and plant extracts is a sustainable alternative to control this pest. The objectives of this study were to select entomopathogenic fungi to control A. woglumi and verify the insecticidal effect of the combined use of the selected fungi and aqueous extracts of castor bean leaves, Ricinus communis L. (Euphorbiaceae). Twenty fungal isolates obtained from field‐collected dead A. woglumi were identified and tested against the insect. Eight were identified as belonging to Fusarium incarnatum‐equiseti species complex, nine to Fusarium fujikuroi species complex, two to Cordyceps javanica (Bally) Kepler, B. Shrestha & Spatafora, and one to genus Akanthomyces. The five isolates that caused the maximum confirmed mortality were tested for compatibility with aqueous castor bean extract at 5, 10, and 20% (wt/vol) concentrations. Qualitative phytochemical analysis showed the presence of alkaloids, glycoside flavonoids, saponins, and tannins in the castor bean extract. Most of the isolates studied caused high confirmed mortality of citrus blackfly. In the compatibility tests, Fusarium volatile Al‐Hatmi, Sand.‐Den., SA Ahmed & de Hoog and Fusarium proliferatum (Matsush.) Nirenberg ex Gerlach & Nirenberg combined with the 5% aqueous extract were the most suitable for testing against A. woglumi. Additive interactions were observed for the combined control agents. The highest value of confirmed mortality (98.2%) was observed for the combination of 5% aqueous castor bean extract and F. volatile (URM 8051), the efficacy of which should be confirmed by field tests.

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Section: Discussionmentioning

confidence: 99%

Entomopathogenicity of fungi in combination with Ricinus communis extract for the control of Aleurocanthus woglumi

Barbosa

Santos

Diniz

et al. 2021

Entomologia Exp Applicata

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“…have also been studied as biological control agents of Hemiptera (Teetor‐Barsch & Roberts, ). The Fusarium incarnatum‐equiseti species complex has been used in Brazil to control D. opuntiae alone (Carneiro‐Leão et al., ) or in combination with natural extracts of Ricinus communis L. and Poincianella pyramidalis (Tul.) L.P. Queiroz with promising results (Santos et al., ).…”

Section: Integrated Pest Managementmentioning

confidence: 99%

“…L.P. Queiroz with promising results (Santos et al., ). A key point for the use of this fungus in IPM is the molecular characterization of the genetic profiles of strains of F. incarnatum‐equiseti , given the considerable differences in the effectiveness as control agents among biotypes collected in the field (Tiago et al., ; Carneiro‐Leão et al., ). The use of Fusarium isolates obtained from D. opuntiae collected in the field and combined with 5% aqueous extract of R. communis stood out as the most promising for the control of D. opuntiae (Santos et al., ).…”

Section: Integrated Pest Managementmentioning

confidence: 99%

Dactylopius opuntiae, a new prickly pear cactus pest in the Mediterranean: an overview

Mazzeo

Nucifora

Russo

et al. 2019

Entomologia Exp Applicata

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The Opuntia cochineal scale or false carmine cochineal scale, Dactylopius opuntiae (Cockerell) (Hemiptera: Dactylopiidae), is spreading rapidly in many countries, especially in the Mediterranean basin, where it has become a serious pest of prickly pear crops, Opuntia ficus‐indica (L.) Miller (Cactaceae). This crop is an important food resource both for humans and livestock. The cochineal was originally used as a biological agent to control cactaceous weeds in many countries where Opuntiaceae had been introduced. Currently, in some countries where the prickly pear is no longer considered a weed but a productive crop, as in the Mediterranean area, D. opuntiae has changed its role from a highly prized biological control agent to the status of serious pest. This paper provides an overview of the current knowledge on D. opuntiae for farmers and stakeholders in order to indicate the most appropriate way to limit or counteract the spread of this pest especially in new cultivated areas.

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“…DNA fingerprinting is an important method for distinguishing differences between organisms of the same species and between organisms of different species from a molecular level. By designing primers specific for the segment of genes that determine the prolongation factor of pest growth and development, followed by a series of molecular marker techniques based on PCR, smaller fragments of pests in flour that are not easily observed with the naked eye can be detected [ 18 , 19 ]. However, there are more bands in DNA fingerprinting and the strength of each band varies, making it more difficult to determine the alleles.…”

Section: Introductionmentioning

confidence: 99%

An Analytical Method Based on Electrochemical Sensor for the Assessment of Insect Infestation in Flour

Zhu

Karimi-Maleh

2021

Biosensors

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Uric acid is an important indicator of the insect infestation assessment in flour. In this work, we propose a method for uric acid detection based on voltammetry. This technique is particularly considered for the physicochemical properties of flour and contains a simple pretreatment process to rapidly achieve extraction and adsorption of uric acid in flour. To achieve specific recognition of uric acid, graphene and poly(3,4-ethylenedioxythiophene) (PEDOT) were used for the adsorption and concentration of uric acid in flour. The adsorbed mixture was immobilized on the surface of a screen-printed electrode for highly sensitive detection of the uric acid. The results showed that electrocatalytic oxidation of uric acid could be achieved after adsorption by graphene and PEDOT. This electrocatalytic reaction allows its oxidation peak to be distinguished from those of other substances that commonly possess electrochemical activity. This voltammetry-based detection method is a portable and disposable analytical method. Because it is simple to operate, requires no professional training, and is inexpensive, it is a field analysis method that can be promoted.

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Dactylopius opuntiae: control by the Fusarium incarnatum–equiseti species complex and confirmation of mortality by DNA fingerprinting

Cited by 20 publications

References 26 publications

Entomopathogenicity of fungi in combination with Ricinus communis extract for the control of Aleurocanthus woglumi

Entomopathogenicity of fungi in combination with Ricinus communis extract for the control of Aleurocanthus woglumi

Dactylopius opuntiae, a new prickly pear cactus pest in the Mediterranean: an overview

An Analytical Method Based on Electrochemical Sensor for the Assessment of Insect Infestation in Flour

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