Dahi containing Lactobacillus acidophilus and Bifidobacterium bifidum improves phagocytic potential of macrophages in aged mice

Abstract

The present study investigated the effect of Dahi containing potential probiotic bacterial strains on macrophage functions in ageing mice. Probiotic Dahi was prepared by co-culturing Dahi bacteria (Lactococcus lactis ssp. cremoris and Lactococcus lactis ssp. lactis biovar diacetylactis) along with Lactobacillus acidophilus LaVK2 (La-Dahi) or combined L. acidophilus LaVK2 and Bifidobacterium bifidum BbVK3 (LaBb-Dahi) in buffalo milk. The effect of ageing on phagocytic function was evaluated on 4 mo, 12 mo and 16 mo old mice. The effect of probiotic Dahi was evaluated for macrophage functions in ageing mice (12 mo old) fed La-Dahi or LaBb-Dahi supplements for 4 months. The production of extracellular superoxide and H 2 O 2 declined in peritoneal macrophages but enhanced in splenic macrophages, while intracellular superoxide declined in both peritoneal and splenic macrophages with ageing in mice. A decline in phagocytic activity of peritoneal macrophages was also observed in aged mice. Supplementation of diet with La-Dahi or LaBb-Dahi for 4 months improved production of reactive oxygen species and phagocytic and adherence indices of peritoneal macrophages in aged mice. These results suggest that oral administration of La-Dahi or LaBb-Dahi has potential to improve immune functions in ageing individuals.

Dietary Supplementation with Probiotic Strain Improves Immune-Health in Aged Mice

“…Several studies have demonstrated that probiotics are able to reduce age-associated changes on phagocytic cells. It was reported that administration of the probiotic strains L. acidophilus LaVK2 or B. bifidum BbVK3 ameliorate age induced deficits in phagocytic activity of peritoneal macrophages with corresponding increase in production of reactive oxygen species [11]. It was also reported that oral administration of L. lactis subsp.…”

Antimicrobial Effect of Probiotics against Common Pathogens

“…Probiotic candidates have been accessed from very diverse habitats including faeces of breastfed human infants [65,69,80,85,95], faeces of healthy adults [9,15,65,70], faeces of elderly [81], faeces of children [25,96], breast milk [42], human saliva [52], vaginal isolates of healthy women [66,75], various fermented foods or beverages including raw or fermented milk [23,35,44], kefir [97], cheese [51,56,98], whey [99], yogurt [16,41], dahi [100,101], other dairy products [25,36,61], sourdough [102], sausages [17], fermented meat [24], kimchi [10,62], maize [25,59], fermented olives [103], Yerba mate [79], ragi [64], soy sauce [86], soil [104], as well as animal origin including rat faeces [71], geese [68], c...…”

Дослідження Процесів Ферментації Та Фізичного Визрівання Вершків У Весняно-Літній Період Року При Виробництві Масла З Пробіотичними Властивостями

“…У наш час у різних країнах світу L. acidophilus вводять у молочну сировину як монокультуру, так і у комплексі з різноманітними видами молочнокислих бактерій для виробництва кисломолочних продуктів (Kaushal and Kansal, 2014). Доведено можливість і доцільність спільного культивування мезофільних молочнокислих лактококів і ацидофільної палички (Dianawati et al, 2013), що дозволяє одержати у продукті досить високу концентрацію життєздатних клітин обох груп мікроорганізмів.…”