Dairy cattle as a bioindicator of exposure to genotoxic substances in a heavily polluted area in Northern Bohemia

Rubeš, Jiřı́; Pokorná, Zuzana; Borkovec, L.; Urbanová, J.; Strnadová, V

doi:10.1016/s0165-1218(97)00032-3

Cited by 16 publications

(5 citation statements)

References 33 publications

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“…Mutagenic tests have been applied to study the chromosome fragility (or stability) on cells exposed both in vivo and in vitro to mutagens or chemicals, suspected to have mutagenic activity (Mrdjanovic et al, 2014;Lovreglio et al, 2014). In particular, the cytogenetic tests we used in the present study have been applied in both human and animal lymphocytes, often revealing a higher chromosome fragility, compared to control groups (in vivo exposure) or control unexposed cells (in vitro exposure) (Rubes et al, 1997;Lioi et al, 1998;Picco et al, 2004;Iannuzzi et al, 2004;Perucatti et al, 2006;Santoro et al, 2008;Bonassi et al, 2008;Di Meo et al, 2011;Genualdo et al, 2012;Siviková and Dianovsky, 2006;Sivikova et al, 2013;Georgieva et al, 2013;Genualdo et al, 2015;Perucatti et al, 2015;Iannuzzi et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic tests reveal no toxicity in lymphocytes of rabbit ( Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene

Perucatti

Genualdo

Pauciullo

et al. 2018

Food and Chemical Toxicology

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Phenylpropanoid glycosides (PPG), like other phenolic compounds, are a powerful antioxidants and the Verbascoside (VB) is one of the most active of them. A previous study, by using in vitro exposure of blood human lymphocytes to Verbascoside, reported a significant increasings of chromosome fragility compared to control. In the present study, four homogeneous groups of rabbits were used to test in vivo the VB and/or Lycopene (LP) by feeding the animals without VB and LP (control), in presence of VB or/and LP for 80 days. Lymphocyte cell cultures were performed in three different times: 0, 40 and 80 days of the experiment and the cytogenetic tests that we used [CA-test (Chromosome Abnormalities in terms of chromosome and chromatid breaks) and Sister Chromatid Exchange (SCE-test)] have revealed no mutagenic effects on chromosomes. Indeed, mean values/cell of CA and SCE decreased during the experiment with some difference among and within groups, with significant decreasing value only for some group. The study shows clear evidence that diets rich in Verbascoside (and/or Lycopene) do not originate any mutagenic activity, resulting no cytotoxic for the animals and, suggesting a possible their use in both animal and human diets.

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Section: Discussionmentioning

confidence: 99%

Cytogenetic tests reveal no toxicity in lymphocytes of rabbit ( Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene

Perucatti

Genualdo

Pauciullo

et al. 2018

Food and Chemical Toxicology

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“…and waste products of industrial plants. [22] The exposure of dairy cattle to genotoxic substances showed a significantly higher count of aberrant cells estimated using cytogenetic analysis of bovine peripheral lymphocytes: [23] the main source of chemical substances was the feeding of roughage and fresh fodder. In livestock, exposure to genotoxic agents may result in mutations, metabolic disorders, immunosuppression and reduced fertility.…”

Section: Discussionmentioning

confidence: 99%

Effect of triazole pesticide formulation on bovine culture cells

Holečková

Šiviková

Dianovský

et al. 2013

Journal of Environmental Science and Health, Part B

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To date, most data about the possible genotoxic effect of triazole pesticides are focused on laboratory animals resulting in limited information on further non-target organisms such as cattle. The objective of the present study was to investigate the effect of triazole (tebuconazole/prothioconazole) fungicide formulation on the induction of chromosomal aberrations (CAs), sister chromatid exchanges (SCEs) and DNA fragmentation in bovine cultured lymphocytes. Our results showed that the fungicide formulation did not induce significant number of CAs in bovine cells after 24 h treatment. Nevertheless, the dose-dependent reduction of mitotic division was observed, with the strongest effect at 30.0 μg mL(-1) in both donors (P < 0.01 and P < 0.001, respectively). Prolonged 48 h exposure caused the increased level of breaks in treated cultures (3.0-15.0 μg mL(-1); P < 0.05) and significant decrease in mitotic index (MI). The tested fungicide failed to produce any statistical changes in the SCE frequency neither after 24 h nor 48 h treatment. However, the significant decline of the proliferation index (PI) was observed after 24 h indicating the fungicide influence on cell cycle kinetics. Prolonged 48 h exposure caused cytotoxicity reflecting in lower PI value relative to control mainly at the highest fungicide concentrations (30.0 μg mL(-1), P < 0.001). Using painting probes for bovine chromosomes 1, 5 and 7 (BTA1, BTA5 and BTA7) only low levels of aneuploidies were detected. Significant increase of polyploidy cells (P < 0.05) was induced by a 3.0 μg mL(-1) dose of the fungicide after 48 h. DNA fragmentation assay didn't reveal the presence of DNA nucleosome ladder in cell cultures at any time (24 h and 48 h) and fungicide concentration.

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“…In particular, SCE is a short-term test for the detection of reciprocal exchanges of DNA between two sister chromatids, involving DNA breakage and subsequent re-junction. The increased frequencies of SCE due to negative conditions, like pollutant exposition, lead to DNA single strand breaks as reported in several studies on livestock populations (Rubes et al, 1997;Di Meo et al, 2000Iannuzzi et al, 2004;Perucatti et al, 2006;Genualdo et al, 2012;Wójcik and Smalec, 2013). The goal of this study is to evaluate the impact of environmental pollutants in some areas of Sardinia Island on the genome of sheep flocks living in this region by using the SCE test.…”

Section: Introductionmentioning

confidence: 89%

Analysis of chromosome damage by sister chromatid exchange (SCE) and redox homeostasis characterization on sheep flocks from Sardinian pasturelands

Genualdo

Perucatti

Pauciullo

et al. 2015

Science of The Total Environment

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Dairy cattle as a bioindicator of exposure to genotoxic substances in a heavily polluted area in Northern Bohemia

Cited by 16 publications

References 33 publications

Cytogenetic tests reveal no toxicity in lymphocytes of rabbit ( Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene

Cytogenetic tests reveal no toxicity in lymphocytes of rabbit ( Oryctolagus cuniculus, 2n=44) feed in presence of verbascoside and/or lycopene

Effect of triazole pesticide formulation on bovine culture cells

Analysis of chromosome damage by sister chromatid exchange (SCE) and redox homeostasis characterization on sheep flocks from Sardinian pasturelands

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