Dalbergiones lower the inflammatory response in oral cells in vitro

Abstract

Objectives Periodontitis is a global health burden that underlines the demand for anti-inflammatory treatment. Dalbergia melanoxylon being a rich source of flavonoids has been widely used in traditional medicine but the potential anti-inflammatory activity of its dalbergiones remains to be shown. Material and methods We have isolated 3′-hydroxy-4,4′-dimethoxydalbergione, 4-methoxydalbergione, and 4′-hydroxy-4-methoxydalbergione from Dalbergia melanoxylon and tested their potential anti-inflammatory activity. Results All dalbergiones are potent inhibitors of an LPS-induced inflammatory response of RAW 264.7 macrophages. This is specified by IL1β and IL6 production, and the p65 nuclear translocation. Consistently, in primary macrophages, the dalbergiones caused an M1-to-M2 polarization switch indicated by the decreased ration of IL1β and IL6 versus arginase 1 and YM1 expression. To implement oral cells, we have used gingival fibroblasts exposed to IL1β and TNFα. Consistently, all dalbergiones reduced the expression of IL6 and IL8 as well as the nuclear translocation of p65. Conclusion These findings increase the accumulating knowledge on dalbergiones and extend it towards its capacity to lower the inflammatory response of oral cells. Clinical relevance These findings are another piece of evidence that supports the use of herbal medicine to potentially lower inflammatory events related to dentistry.

Prenylated Isoflavanones with Antimicrobial Potential from the Root Bark of Dalbergia melanoxylon

“…Although we could not demonstrate anthelmintic activity for the crude extract of D. melanoxylon, mild anthelmintic effects of prenylated isoflavonoids have been reported [48]. Neoflavonoids (represented e.g., by methyl dalbergin (11) and dalbergin (12)) were not included in the biological testing in our study but were previously shown to possess osteogenic properties [37] whereas structurally related dalbergiones from D. melanoxylon exhibited anti-inflammatory effects [49]. Prenylated flavonoids and isoflavonoids play important roles in the defense strategy of plants by protecting them against diseases through a broad inhibition profile against bacteria and fungi [50].…”

“…Neoflavonoids (represented e.g. , by methyl dalbergin ( 11 ) and dalbergin ( 12 )) were not included in the biological testing in our study but were previously shown to possess osteogenic properties [ 37 ] whereas structurally related dalbergiones from D. melanoxylon exhibited anti-inflammatory effects [ 49 ].…”