Danio rerio as a model in aquatic toxicology and sediment research

Hollert, Henner; Keiter, Steffen

doi:10.1007/s11356-015-5362-1

Cited by 24 publications

(10 citation statements)

References 25 publications

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“…However, very little is known about the regulation and function of stress granules formation in non-mammalian vertebrates. The zebrafish ( Danio rerio ) is a fresh-water fish of the cyprinid family, and it represents one of the most popular and versatile genetic models for environmental and human disease studies 21,22 . However, in zebrafish, only limited information concerning the presence and regulation of SG-like structures is available 23–25 .…”

Section: Introductionmentioning

confidence: 99%

YB-1 recruitment to stress granules in zebrafish cells reveals a differential adaptive response to stress

Guarino

Mauro

Ruggiero

et al. 2019

Sci Rep

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The survival of cells exposed to adverse environmental conditions entails various alterations in cellular function including major changes in the transcriptome as well as a radical reprogramming of protein translation. While in mammals this process has been extensively studied, stress responses in non-mammalian vertebrates remain poorly understood. One of the key cellular responses to many different types of stressors is the transient generation of structures called stress granules (SGs). These represent cytoplasmic foci where untranslated mRNAs are sorted or processed for re-initiation, degradation, or packaging into mRNPs. Here, using the evolutionarily conserved Y-box binding protein 1 (YB-1) and G3BP1 as markers, we have studied the formation of stress granules in zebrafish ( D. rerio ) in response to different environmental stressors. We show that following heat shock, zebrafish cells, like mammalian cells, form stress granules which contain both YB-1 and G3BP1 proteins. Moreover, zfYB-1 knockdown compromises cell viability, as well as recruitment of G3BP1 into SGs, under heat shock conditions highlighting the essential role played by YB-1 in SG assembly and cell survival. However, zebrafish PAC2 cells do not assemble YB-1-positive stress granules upon oxidative stress induced by arsenite, copper or hydrogen peroxide treatment. This contrasts with the situation in human cells where SG formation is robustly induced by exposure to oxidative stressors. Thus, our findings point to fundamental differences in the mechanisms whereby mammalian and zebrafish cells respond to oxidative stress.

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Section: Introductionmentioning

confidence: 99%

YB-1 recruitment to stress granules in zebrafish cells reveals a differential adaptive response to stress

Guarino

Mauro

Ruggiero

et al. 2019

Sci Rep

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“…Table 1 gives an overview on the toxicological endpoints registered during exposure. Endpoints were selected according to their toxicological relevance, and their characterization in previous studies [37].…”

Section: Danio Rerio Multi-endpoints Toxicity Assay With Sediments Comentioning

confidence: 99%

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

et al. 2019

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Background: The toxicological characterization of sediments is an essential task to monitor the quality of aquatic environments. Many hazardous pollutants may accumulate in sediments and pose a risk to the aquatic community. The present study provides an attempt to integrate a diagnostic whole mixture assessment workflow based on a slightly modified Danio rerio embryo acute toxicity test with chemical characterization. Danio rerio embryos were directly exposed to sieved sediment (≤ 63 μm) for 96 h. Sediment samples were collected from three polluted sites (Kramfors, Sundsvall and Örnsköldsvik) in the Gulf of Bothnia (Sweden) which are characterized by a long history of pulp and paper industry impact. Effect data were supported by chemical analyses of 237 organic pollutants and 30 trace elements. Results: The results show that malformations and neurotoxic compounds are the main drivers of differentiation in chemical and effects analyses, respectively. Specific spinal cord malformations and delayed hatching were observed only in sediments from Kramfors while light hyperactivity was seen only after exposure to sediments from Sundsvall. Conclusions: Our experiments demonstrate that specific chemical profiles lead to specific effect patterns in Danio rerio embryos. In fact, behavioral endpoints could help detect the exposure to neurotoxins, and the observation of body malformations seems to be a potential tool for the identification of site-specific pollutants as polychlorinated biphenyl (PCBs), brominated flame retardants (BFRs) and several pesticides. Overall, results show the suitability of Danio rerio embryos for the fast screening of sediment samples.

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“…Considering that treated wastewaters exhibit usually low or absent acute toxicity, but can contain complex mixture of micropollutants at low concentrations prone to result in sub-lethal and delayed effects [13], this study aimed to develop and implement a sensitive test battery using zebrafish embryos and in vitro tests This test battery enabling to explore different biological effects was applied to the toxicity evaluation of differently treated effluents at a pilot scale fed by a mixture of hospital and urban wastewaters. In a first campaign, the raw mixed effluent was treated through a moving bed biofilm reactor (MBBR) followed by a tertiary ozonation step with a rather high ozone dose.…”

Section: Introductionmentioning

confidence: 99%

Assessment of Ozone or Not-Treated Wastewater Ecotoxicity Using Mechanism-Based and Zebrafish Embryo Bioassays

Wigh¹,

Aı̈t-Aı̈ssa²,

Creusot³

et al. 2018

JEP

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Toxicity of wastewater treatment plant (WWTP) effluents is usually assessed with standardized bioassays, as e.g. the Fish Embryo Toxicity test (FET), but assessment of sub-lethal toxic effects requires to develop more adapted tests. The present work aimed to add the recording of several sub-lethal endpoints in exposed zebrafish embryo-larval stages in order to increase the sensitivity of residual toxicity evaluation of WWTP effluents using a semi-quantitative method (iFET score). This approach was complemented with the genotoxicity assessment on the exposed embryos, a sub-acute hazard particularly relevant to evaluate a potential chronic toxicity risk of low and multi-contaminated environmental matrices. Additionally, endocrine activities of effluents were quantified using human reporter cell lines. This test battery was applied to the assessment of the residual toxicity of five biological treatment effluents, further treated or not using various ozonation treatments intended to improve pharmaceutical compounds removal. Acute toxicity towards zebrafish embryos was very low. However, iFET score approach proposed was able to reveal residual toxicity through the presence of developmental abnormalities in all samples tested. Additionally, a low residual genotoxicity was measured in embryos exposed to two of the WWTP effluents when all excepted one exhibited a residual

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Danio rerio as a model in aquatic toxicology and sediment research

Cited by 24 publications

References 25 publications

YB-1 recruitment to stress granules in zebrafish cells reveals a differential adaptive response to stress

YB-1 recruitment to stress granules in zebrafish cells reveals a differential adaptive response to stress

Toxicity and neurotoxicity profiling of contaminated sediments from Gulf of Bothnia (Sweden): a multi-endpoint assay with Zebrafish embryos

Assessment of Ozone or Not-Treated Wastewater Ecotoxicity Using Mechanism-Based and Zebrafish Embryo Bioassays

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