Daño en el ADN de linfocitos humanos por efecto de cloroquina

Abstract

Citar como: Amésquita L, Cruz-Briceño MN, Prieto Z. Daño en el ADN de linfocitos humanos por efecto de cloroquina. Rev Peru Med Exp Salud Publica. 2018;35(3):471-5. RESUMENEl objetivo del estudio fue evaluar el efecto de cloroquina (CQ) en linfocitos humanos a través del ensayo cometa. Los linfocitos fueron aislados de muestras de sangre periférica obtenidas de tres donantes sanos, no fumadores de 24 a 30 años. Los linfocitos aislados fueron expuestos durante una hora a diversos tratamientos: peróxido de hidrógeno 2,5 % (control positivo), buffer fosfato salino 1X (control negativo) y cloroquina a concentraciones de 0 µg/ml, 0,25 µg/ml; 5 µg/ml y 300 µg/ml. Se registró los promedios del porcentaje de ADN en la cola del cometa, momento de la cola y momento de la cola de Olive, encontrándose diferencias significativas entre las diversas concentraciones de CQ (p<0,01). Asimismo, la magnitud de daño del ADN se incrementó en función de la concentración de CQ. Se demostró el efecto genotóxico de CQ en linfocitos humanos expuestos in vitro. ABSTRACTThe objective of the study was to evaluate the effect of chloroquine (CQ) on human lymphocytes through the Comet Assay. Lymphocytes were isolated from peripheral blood samples obtained from three healthy, non-smoking donors aged 24 to 30 years. The isolated lymphocytes were exposed for one hour to various treatments: hydrogen peroxide 2.5% (positive control), saline buffer phosphate 1X (negative control) and chloroquine at concentrations of 0 µg/ml, 0.25 µg/ml; 5 µg/ml and 300 µg/ml. The averages of the percentage of DNA in the comet tail, moment of tail and moment of Olive's tail were recorded, with significant differences between the different concentrations of CQ (p<0.01). Also, the magnitude of DNA damage was increased as a function of the CQ concentration. The genotoxic effect of CQ was demonstrated in human lymphocytes exposed in vitro.

Treatment of condylomata acuminata caused by low-risk human papillomavirus with chloroquine phosphate gel

“…This allows the virus to 'control' the cell and reproduce copies of itself. Since the HPV E6 protein degrades p53, it allows the infected cell to replicate itself and prevents apoptosis (32). In the present study, chloroquine phosphate gel increased the expression of p53 protein and a high dose resulted in the necrosis of the epithelial cells of the CA, possibly due to the expression of the p53 protein.…”

“…The HPVE6 protein binds to p53 and degrades or inactivates p53, thereby inhibiting the stabilization of p53. p53 stabilization affects HPVE7 expression (32). This allows the virus to 'control' the cell and reproduce copies of itself.…”