DAPK1 may be a potential biomarker for arterial aneurysm in clinical treatment and activated inflammation levels in arterial aneurysm through NLRP3 inflammasome by Beclin1

Wu, Senyan; Lu, Wei; Cheng, Guobing; Wu, Jiawen; Liao, Shengyou; Qin, Hu; Li, Xiaoyang; Jiang, Buping

doi:10.1177/09603271211041667

Cited by 8 publications

(12 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Average daily caloric of liquid intake per mouse was monitored and calculated in Figure S1. After 12 h of fasting, mice were anesthetized with pentobarbital solution (50 mg/kg body weight) ( 16 ). Livers and adiposes were dissected quickly, washed twice with saline, blotted dry on a filter paper, and their weights were measured.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol

Guo

Zhu²,

Chen³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

Dietary fat composition is closely associated with the pathological development of alcoholic liver disease (ALD). Fat enriched with saturated fatty acids protects whereas with polyunsaturated fatty acids aggravates alcohol-induced liver injury. However, limited study has addressed how monounsaturated fatty acids (MUFAs) determines the pathological process of ALD. Our study was conducted to evaluate the effect of MUFAs-enriched-camellia seed oil (CSO) on alcohol-induced liver injury. The ALD model was established by feeding C57BL/6 mice with Lieber-DeCarli diet, and with either CSO or polyunsaturated fatty acids (PUFAs)-enriched-corn oil (CO) as fat source. After 4-week-intervention, CSO-feed rescued alcohol-induced liver injury compared to CO-feed, evidenced by measurements of plasma ALT activity, H&E stain, and hepatic cleaved-Caspase-3 expression. Besides, CSO-feed alleviated alcohol-induced oxidative stress, associated with NRF2 and Hif-1α expressions improvement. The reduction of F4/80 immunostaining and the decreased expressions of hepatic TNF-α and IL-6 suggested CSO-feed improved alcohol-induced inflammation. The mechanistic analysis showed that the inhibition of ASK1 and MAPKs might contribute to CSO-protected liver injury. Notably, we observed CSO-feed relieved the gut microbiota disturbance with the decreased Firmicutes and Turicibater, and the increased Bacteroidota, Alloprevotella, and Bacteroides, and reduced circulatory endotoxin level and lipolysis of adipose tissue, which are the known pathogenic factors in alcohol-induced liver injury. Unexpectedly, CSO induced more hepatic steatosis than CO-feed. In conclusion, CSO attenuated chronic alcohol consumption-induced liver injury but enhanced hepatic steatosis. CSO could be a potential dietary choice for alcoholic individuals with liver injury.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Mice were kept in an environment of 55 ± 5% relative humidity, 23 ± 2 • C, and 12 h light and dark cycles. After 1-week acclimatization, mice were divided into (16). Livers and adiposes were dissected quickly, washed twice with saline, blotted dry on a filter paper, and their weights were measured.…”

Section: Animals Experimental Design and Sample Preparationmentioning

confidence: 99%

Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol

Guo

Zhu²,

Chen³

et al. 2022

Front. Nutr.

View full text Add to dashboard Cite

show abstract

“…DAPK1 is methylated at CpG islands in several tumors, and based on this view it can be used as a biomarker for cancer detection and treatment . In addition, promoter hypermethylation leads to the silencing or loss of expression of DAPK1, which has been detected in several tumor cells. , What’s more, poor clinical prognosis and advanced disease in nonsmall cell lung cancer (NSCLC) patients are closely related to DAPK1 promoter hypermethylation. − Furthermore, studies have shown that lymph node involvement and DAPK1 promoter hypermethylation are involved in the development of gastric cancer (GC) and head and neck squamous cell carcinoma (HNSCC) , (Table ).…”

Section: The Dapk Family In Diseasesmentioning

confidence: 99%

Targeting Death-Associated Protein Kinases for Treatment of Human Diseases: Recent Advances and Future Directions

et al. 2023

View full text Add to dashboard Cite

The death-associated protein kinase (DAPK) family is a member of the calcium/calmodulin-regulated serine/threonine protein kinase family, and studies have shown that its role, as its name suggests, is mainly to regulate cell death. The DAPK family comprises five members, including DAPK1, DAPK2, DAPK3, DRAK1 and DRAK2, which show high homology in the common N-terminal kinase domain but differ in the extra-catalytic domain. Notably, previous research has suggested that the DAPK family plays an essential role in both the development and regulation of human diseases. However, only a few small-molecule inhibitors have been reported. In this Perspective, we mainly discuss the structure, biological function, and role of DAPKs in diseases and the currently discovered small-molecule inhibitors, providing valuable information for the development of the DAPK field.

show abstract

“…Although the impact of the mitochondrial disorder on the induction and maintenance of spinal oxidative stress and inflammatory cytokine accumulation has previously been reported, the underlying mechanism remains unclear [ 1 , 2 ]. AMP-activated protein kinase (AMPK), a strongly conserved and highly expressed eukaryotic protein serine/threonine kinase, is capable of sensing changes in body energy and has a crucial role in modulating mitochondrial function [ 3 ], combating oxidative stress and inflammation [ 4 ]. DEX, an α 2-adrenoceptor agonist, has calming, anxiolytic, and antalgic properties with minimal respiratory inhibition [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Mei

Chen

et al. 2022

Disease Markers

View full text Add to dashboard Cite

Neuropathic pain (NPP) is a debilitating clinical condition that presently has few effective treatments. NPP is caused by uncontrolled central oxidative stress and inflammation. Preliminary studies indicate that dexmedetomidine (DEX), an agonist of the alpha-2 adrenergic receptor, is beneficial for treating NPP. In this paper, the effects of administering DEX around injured nerves in a chronic constriction injury- (CCI-) induced neuropathic pain mouse model are investigated. According to the results, the perineural DEX significantly reversed the decline in the mechanical threshold and thermal latency in CCI mice ( p < 0.001 ). In the peripherally affected ischiadic nerve, the perineuronal DEX upregulated the expressions of pAMPK, OPA1, and SNPH but not Drp1 or KIF5B. The aforementioned effects of administering DEX can be partially reversed by compound C, a selective and reversible inhibitor of AMP-activated protein kinase (AMPK). Furthermore, it was found that perineural DEX significantly inhibited the CCI-induced upregulation of the immediate early gene c-Fos, overexpression of the inflammatory factors tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6), attenuation of the NADH dehydrogenase complexes I, II, III, and IV, and the repression of ATP, SOD, and GSH in the dorsal horn of the spinal cord (DHSC) ( p < 0.01 ). These findings indicate that perineuronal DEX protected the injured ischiadic nerves and attenuated neuropathic pain via AMPK activation to improve energy supply in the peripheral injured nerves, alleviate the inflammatory factor release, and inhibit oxidative stress in the DHSC.

show abstract

DAPK1 may be a potential biomarker for arterial aneurysm in clinical treatment and activated inflammation levels in arterial aneurysm through NLRP3 inflammasome by Beclin1

Cited by 8 publications

References 27 publications

Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol

Dietary camellia seed oil attenuates liver injury in mice chronically exposed to alcohol

Targeting Death-Associated Protein Kinases for Treatment of Human Diseases: Recent Advances and Future Directions

Perisciatic Nerve Dexmedetomidine Alleviates Spinal Oxidative Stress and Improves Peripheral Mitochondrial Dynamic Equilibrium in a Neuropathic Pain Mouse Model in an AMPK-Dependent Manner

Contact Info

Product

Resources

About