Dark fermentative hydrogen production by the unicellular halotolerant cyanobacterium Aphanothece halophytica grown in seawater

Taikhao, Samart; Incharoensakdi, Aran; Phunpruch, Saranya

doi:10.1007/s10811-014-0292-8

Cited by 27 publications

(24 citation statements)

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“…Since A . halophytica can grow in natural seawater supplemented with 1.76 mM NaNO 3 [7], it would be useful if this cyanobacterium grown in natural seawater will produce long-term of H 2 photohydrogen by using PSII inhibitors. This study needs further investigation.…”

Section: Discussionmentioning

confidence: 99%

“…The bidirectional hydrogenase activity in the A. halophytica sample was determined after cells were incubated with various concentrations of CCCP and DCMU under the light for 2 h. Bidirectional hydrogenase activity was measured in the presence of dithionite-reduced methyl viologen. The assay contained 1 mL of cells in suspension and 1 mL of 25 mM phosphate buffer (pH 7.0) containing 2.5 mM methyl viologen and 10 mM sodium dithionite [7]. The reaction mixture was incubated under dark anaerobic conditions at 25°C for 15 min before H 2 production was measured using gas chromatography, under previously described conditions [7].…”

Section: Methodsmentioning

confidence: 99%

“…The assay contained 1 mL of cells in suspension and 1 mL of 25 mM phosphate buffer (pH 7.0) containing 2.5 mM methyl viologen and 10 mM sodium dithionite [7]. The reaction mixture was incubated under dark anaerobic conditions at 25°C for 15 min before H 2 production was measured using gas chromatography, under previously described conditions [7]. Bidirectional hydrogenase activity was calculated in terms of μ mol H 2 g −1 dry weight min −1 .…”

Section: Methodsmentioning

confidence: 99%

“…The unicellular cyanobacterium Aphanothece halophytica is a halotolerant microorganism that can grow in a wide range of salinity from 0.25 to 3.0 M NaCl [5]. A. halophytica produces a large amount of dark fermentative H 2 compared with other marine cyanobacteria [6, 7]. H 2 production by A. halophytica is catalyzed by bidirectional hydrogenase and occurs particularly under nitrogen-deprived and dark anaerobic conditions [6–8].…”

Section: Introductionmentioning

confidence: 99%

“…Hydrogenase is the only enzyme that catalyzes both H 2 uptake and H 2 production in this organism [8]. Due to the high sensitivity of bidirectional hydrogenase to oxygen (O 2 ) [9], which is the main product when photosystem II (PSII) activity splits a water molecule, H 2 production by A. halophytica decreases in the light [7]. To enhance H 2 production by A. halophytica , O 2 must be removed.…”

Section: Introductionmentioning

confidence: 99%

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Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Pansook

Incharoensakdi

Phunpruch

2019

The Scientific World Journal

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The unicellular halotolerant cyanobacterium Aphanothece halophytica is a potential dark fermentative producer of molecular hydrogen (H2) that produces very little H2 under illumination. One factor limiting the H2 photoproduction of this cyanobacterium is an inhibition of bidirectional hydrogenase activity by oxygen (O2) obtained from splitting water molecules via photosystem II activity. The present study aimed to investigate the effects of the photosystem II inhibitors carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU) on H2 production of A. halophytica under light and dark conditions and on photosynthetic and respiratory activities. The results showed that A. halophytica treated with CCCP and DCMU produced H2 at three to five times the rate of untreated cells, when exposed to light. The highest H2 photoproduction rates, 2.26 ± 0.24 and 3.63 ± 0.26 μmol H2 g−1 dry weight h−1, were found in cells treated with 0.5 μM CCCP and 50 μM DCMU, respectively. Without inhibitor treatment, A. halophytica incubated in the dark showed a significant increase in H2 production compared with cells that were incubated in the light. Only CCCP treatment increased H2 production of A. halophytica during dark incubation, because CCCP functions as an uncoupling agent of oxidative phosphorylation. The highest dark fermentative H2 production rate of 39.50 ± 2.13 μmol H2 g−1 dry weight h−1 was found in cells treated with 0.5 μM CCCP after 2 h of dark incubation. Under illumination, CCCP and DCMU inhibited chlorophyll fluorescence, resulting in a low level of O2, which promoted bidirectional hydrogenase activity in A. halophytica cells. In addition, only CCCP enhanced the respiration rate, further reducing the O2 level. In contrast, DCMU reduced the respiration rate in A. halophytica.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Pansook

Incharoensakdi

Phunpruch

2019

The Scientific World Journal

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Generation of Hydrogen Using Cyanobacteria

Dutra,

Schneider,

Dias

et al. 2024

Solid‐Gaseous Biofuels Production

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Co‐production of bioethanol and commercially important exopolysaccharides from the marine cyanobacterium Synechococcus elongatus BDU 10144 in a novel low‐cost seawater‐fertilizer‐based medium

Chandra

Mallick

2022

Intl J of Energy Research

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Summary The production of bioethanol by cyanobacteria can become economically sustainable if other valuable commercial products, such as exopolysaccharides (EPS), are produced simultaneously. This study investigates the feasibility of producing bioethanol and the commercially important EPS from a non‐nitrogen fixing marine cyanobacterium, Synechococcus elongatus BDU 10144 in a novel low‐cost seawater‐fertilizer medium for the development of a biorefinery strategy. Herein, agricultural fertilizer diammonium phosphate (DAP) (nitrogen source), magnesium sulfate (MgSO4) and potassium were optimized by the central composite design to produce maximum biomass and carbohydrate accretion. The optimal physical conditions for attaining maximum growth and carbohydrate accumulation were determined to be pH 10, 50 μmol m−2 s−1 (light intensity) and 25°C. Further, different concentrations of seawater were mixed with the optimized fertilizer‐salts, and it was observed that 70% of seawater mixed with the optimized fertilizer‐salts (hereafter FSW medium) was an optimum condition for growing the test cyanobacterium, which ultimately reduced the cost of the medium by >40 times compared to the standard artificial seawater nutrient (ASN‐III) medium. Under the optimal conditions, the maximum biomass (1.79 g/L) and carbohydrate (702.4 mg/L) yield obtained were ~1.7 and 2.2 times higher than the ASN‐III. The yield of bioethanol was noted to be 318.1 mg/L, which was ~2.3 times greater than ASN‐III. The commercially important EPS was obtained from the discarded supernatant (0.28 g/L), which was ~1.6 times higher in the FSW medium. Thus, the present investigation paves a way forward for mass cultivation of the S. elongatus BDU 1044 in the novel FSW medium for the cost‐efficient and sustainable development of a biorefinery concept.

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Dark fermentative hydrogen production by the unicellular halotolerant cyanobacterium Aphanothece halophytica grown in seawater

Cited by 27 publications

References 27 publications

Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Effects of the Photosystem II Inhibitors CCCP and DCMU on Hydrogen Production by the Unicellular Halotolerant CyanobacteriumAphanothece halophytica

Generation of Hydrogen Using Cyanobacteria

Co‐production of bioethanol and commercially important exopolysaccharides from the marine cyanobacterium Synechococcus elongatus BDU 10144 in a novel low‐cost seawater‐fertilizer‐based medium

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