DART MS Based Chemical Profiling for Therapeutic Potential of Piper Betle Landraces

Abstract

Piper betle Linn.leaves are traditionally used as a folk medicine in India and other Asiatic countries. Twenty-one P. betle landraces were analyzed using a Direct Analysis in Real Time (DART) mass spectral technique and evaluated on the basis of molecules detected in the leaves. Clustering of landraces based on three well known biologically active phenols (m/z 151,165,193) showed two broad groups with high and low phenol contents suggesting differences in their therapeutic potential. Findings of this study could be useful in rapid screening of the landraces for determining their medicinal potential and optimum utilization of the bioresource.

A rapid analytical method for characterization and simultaneous quantitative determination of phytoconstituents in Piper betle landraces using UPLC-ESI-MS/MS

“…22 Previously GC-MS and DART-MS methods were reported for the analysis of chemical constituents of betel leaf. [23][24][25][26][27] GC-MS studies on P. betle leaf oil showed the presence of safrole, allylpyrocatechol-3,4-diacetate, eugenol and eugenyl acetate as the major components and their quantitative variation in different landraces due to the difference in geographical conditions of growth. [23][24][25] P. betle landraces were also analyzed using the Direct Analysis in Real Time (DART) mass spectral technique which showed the presence of mainly terpenes and phenols in betle leaves.…”

“…Some variations were observed in the composition and percentage of constituents. 26,27 Analytical methods based on HPLC-PDA and HPTLC have been reported for the determination of eugenol and eugenyl acetate in the ower and leaf of P. betle. 2,28 Eugenol is also quantied in plasma using tandem mass spectrometry coupled with liquid chromatography.…”