Data from quantitative serum proteomic analysis after laparoscopic gastric plication

Abstract

Bariatric surgery is an effective treatment for morbid obesity with a sustained weight loss and improvements in metabolic syndrome. We present a label free quantitative shotgun proteomics approach to analyze the serum proteome of obese people who underwent Laparoscopic Gastric Plication (LGP) as a new bariatric surgery. Pre-surgery serum samples of obese individuals were compared with the serum of the same subjects 1–2 months post-surgery (T1) and 4–5 months post-surgery (T2). The data provide a list of 224 quantifiable proteins with at least two unique peptides that were quantifiable in at least 70% of samples. Gene ontology biological processes and molecular functions of differentially regulated proteins between pre- and post-surgery samples were investigated using WebGestalt online tool. In addition, molecular networks of differentially abundant proteins were determined through Ingenuity Pathway Analysis (IPA) software. This report is related to the research article entitled “Serum proteome changes and accelerated reduction of fat mass after Laparoscopic Gastric Plication in morbidly obese patients” (Savedoroudi et al. [1]). Proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository through the identifier PXD010528.

Serum proteome changes and accelerated reduction of fat mass after laparoscopic gastric plication in morbidly obese patients

“…proteins to ensure only proteins with high quality quantification were retained, 224 proteins met our quantitation criteria (Supplementary Table 1 in Ref [24]).…”

“…Gene ontology (GO) overrepresentation analysis for biological process and molecular function was conducted with WebGestalt to gain a biological view of significantly changing proteins [22]. The complete list of enriched GO terms is reported in Table 2 and Table 3 for T1 and T2 in Ref [24], respectively. The GO analysis revealed that most of the significantly changing proteins at T1 post-surgery have molecular functions such as lipid transporter activity, steroid binding and lipid binding.…”

“…Five significant networks were identified when presurgery subjects were compared with T1. The top enriched network defined as "Lipid Metabolism, Molecular Transport, Small Molecule Biochemistry" with a score of 45, containing 4 in Ref [24].…”