Data fusion from several densitometric modes in fingerprinting of 70 grass species

Simultaneous component analysis as an interesting preliminary data analysis method in GC-MS – An example of headspace screening of Polish grasses

“…Five species: GlMax, LoMul, HoJub, BrSec and BrTec have highest values of this component. Although it is connected with trend independent of the phytochemical content, their outliance is similar to our previous study on these grasses, done by thin-layer chromatography [37]. Therefore, they truly deserve further investigation.…”

“…However, this method resulted in chromatogram with unacceptable sensitivity. Therefore, we decided to perform liquid extraction, evaporate the extract and dissolve in methanol just like in our previous TLC work [37]. Although there is a risk of the loss of most volatile compounds, each evaporated extract had very strong herbal odor and the corresponding chromatogram presented much more peaks.…”

Novel Hyperspectral Analysis of Thin-Layer Chromatographic Plates—An Application to Fingerprinting of 70 Polish Grasses

“…As our second idea was to investigate an idea for infrared extension to video scanning, we realized that grasses are a good group for checking the hyperspectral extension of video scanning. Our previous thin-layer chromatography analysis [34] proved that they contain constituents glowing under ultraviolet light with various colors. Therefore, it can be expected that some spots will also glow in the infrared part of the spectrum, which will, in this case, add new information to the fingerprint.…”

“…The purpose of this idea is to classify the species according to the similarity of their chemical composition and to identify some interesting plants for further, more detailed research in the future. In our previous research [34], we performed fingerprinting with densitometric scanning and visually identified some unknown markers. The identification of them requires further research and mass spectrometry study, as there is no strict literature reference to discuss (only several species were analyzed by this method in the earlier research; for a literature survey, see Table 1 in [34]).…”

“…In our previous research [34], we performed fingerprinting with densitometric scanning and visually identified some unknown markers. The identification of them requires further research and mass spectrometry study, as there is no strict literature reference to discuss (only several species were analyzed by this method in the earlier research; for a literature survey, see Table 1 in [34]).…”