Data on protein abundance alteration induced by chronic exercise in mdx mice model of Duchenne muscular dystrophy and potential modulation by apocynin and taurine

Abstract

Here we present original data related to the research paper entitled “Proteome analysis in dystrophic mdx mouse muscle reveals a drastic alteration of Key Metabolic and Contractile Proteins after chronic exercise and the potential modulation by anti-oxidant compounds” (Gamberi et al., 2018) [1]. The dystrophin-deficient mdx mouse is the most common animal model for Duchenne muscular dystrophy. The mdx mice phenotype of the disorder is milder than in human sufferers and it can be worsened by chronic treadmill exercise. Apocynin and taurine are two antioxidant compounds proved to be beneficial on some pathology related parameters (Schröder and Schoser, 2009) [2]. This article reports the detailed proteomic data on protein abundance alterations, in tibialis anterior muscle of mdx mice, induced by chronic exercise protocol. A selected group of mdx mice was also treated with apocynin and taurine during this protocol. Detailed MS data, comparison between mdx vs wild type, exercised mdx vs wild type, and complete analysis of spot variation are provided. Furthermore, in wild type mice subjected to the same exercise protocol, the abundance of key proteins, resulted modified in exercised mdx, were analyzed by western blot.

Proteome analysis in dystrophic mdx mouse muscle reveals a drastic alteration of key metabolic and contractile proteins after chronic exercise and the potential modulation by anti-oxidant compounds

“…MS/MS analysis was carried out by using an Ultraflex III MALDI-TOF/TOF mass spectrometer (Bruker Daltonics) and the results were reported Table 1 in ref. [27].…”

“…In Figure 1 and 2 in ref. [27], histograms and picture representing spot abundance variation between each condition are reported.…”

“…In fact, checking the apparent molecular weight and the peptide coverage, we observed that these spots have an apparent molecular weight lower than the one expected and furthermore, the peptide coverage is limited to the N-terminus of the protein, suggesting an activation of proteolysis (Table 2 in ref. [27]). It is also interesting to note an increased level of two spots, corresponding to Tnnt3, which showed a shift in pI value, indicating that exercise increased the level of a Tnn3 isoform harbouring post-translational modification.…”

“…In Table 3 in ref [27] we also reported the differentially abundant proteins between mdx exe and wt.…”

“…Concerning this enzyme, it must be highlighted that several spots (55, 56, 57, 58, 59, 60) presented an experimental molecular weight lower than the one expected (Table 1) indicating that the increased level of this protein (observed for spots 61, 62,64, 65, 67 and 69), is also combined with and increased proteolysis. The protein peptide coverage of these spots, Table 2 of ref [27], indicates that they correspond to aminoterminal fragment of the protein.…”