Data on the Amphidinium carterae Dn241EHU isolation and morphological and molecular characterization

Seoane, Sergio; Molina-Miras, A.; López-Rosales, L.; Sánchez-Mirón, A.; Cerón-García, M.C.; Camacho, F. Garcı́a; Madariaga, Iosu; Molina-Grima, E.

doi:10.1016/j.dib.2018.07.036

Cited by 7 publications

(2 citation statements)

References 7 publications

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“…The marine microalga used in this study was Amphidinium carterae Dn241EHU, which is deposited in the microalgae culture collection of the Plant Biology and Ecology Department of the University of the Basque Country. The morphological characteristics and genetic identification of this strain have been previously reported . Inoculum was grown in f/2 medium as described elsewhere …”

Section: Materials and Methodsmentioning

confidence: 99%

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Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Abreu

Molina-Miras

Aguilera-Sáez

et al. 2019

J. Agric. Food Chem.

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This study assessed the feasibility of an NMR metabolomics approach coupled to multivariate data analysis to monitor the naturally present or stresses-elicited metabolites from a long-term (>170 days) culture of the dinoflagellate marine microalgae Amphidinium carterae grown in a fiberglass paddlewheel-driven raceway photobioreactor. Metabolic contents, in particular, in two members of the amphidinol family, amphidinol A and its 7-sulfate derivative amphidinol B (referred as APDs), and other compounds of interest (fatty acids, carotenoids, oxylipins, etc.) were evaluated by altering concentration levels of the f/2 medium nutrients and daily mean irradiance. Operating with a 24 h sinusoidal light cycle allowed a 3-fold increase in APD production, which was also detected by an increase in hemolytic activity of the methanolic extract of A. carterae biomass. The presence of APDs was consistent with the antitumoral activity measured in the methanolic extracts of the biomass. Increased daily irradiance was accompanied by a general decrease in pigments and an increase in SFAs (saturated fatty acids), MUFAs (monounsaturated fatty acids), and DHA (docosahexaenoic acid), while increased nutrient availability lead to an increase in sugar, amino acid, and PUFA ω-3 contents and pigments and a decrease in SFAs and MUFAs. NMR-based metabolomics is shown to be a fast and suitable method to accompany the production of APD and bioactive compounds without the need of tedious isolation methods and bioassays. The two APD compounds were chemically identified by spectroscopic NMR and spectrometric ESI-IT MS (electrospray ionization ion trap mass spectrometry) and ESI-TOF MS (ESI time-of-flight mass spectrometry) methods.

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Section: Materials and Methodsmentioning

confidence: 99%

“…The morphological characteristics and genetic identification of this strain have been previously reported. 17 Inoculum was grown in f/2 medium as described elsewhere. 13 Production of Microalgal Biomass.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Abreu

Molina-Miras

Aguilera-Sáez

et al. 2019

J. Agric. Food Chem.

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Exploring the potential of epigenetic chemicals to increase metabolite production in the dinoflagellate microalga Amphidinium carterae

Kichouh-Aiadi,

Gallardo-Rodríguez,

Cerón-García

et al. 2024

J Appl Phycol

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This study explores the use of chemical epigenetic modifiers to enhance the production of bioactive molecules in the dinoflagellate Amphidinium carterae. It was selected for its known ability to produce amphidinols with various pharmacological and agro-industrial applications. Growth inhibition, photosynthetic yield reduction, and stress-related variables were observed in response to different treatments using 5-azacitidine, hydroxamic suberoylanilide acid, metyrapone, tricyclazole, sodium butyrate, and jasplakinolide. Although no evidence of amphidinols overproduction was found, hydroxamic suberoylanilide acid and tricyclazole showed potential for stimulating the synthesis of antioxidant carotenoids such as peridinin and their derivatives. An increase of around 200% and 170% relative to control was observed at the highest non-lethal concentration. More research is required to elucidate the mechanisms underlying this response and to optimize production conditions. This pioneering research opens up new avenues for the biotechnological exploitation of dinoflagellate microalgae.

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A new approach to finding optimal centrifugation conditions for shear-sensitive microalgae

et al. 2019

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Data on the Amphidinium carterae Dn241EHU isolation and morphological and molecular characterization

Cited by 7 publications

References 7 publications

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Production of Amphidinols and Other Bioproducts of Interest by the Marine Microalga Amphidinium carterae Unraveled by Nuclear Magnetic Resonance Metabolomics Approach Coupled to Multivariate Data Analysis

Exploring the potential of epigenetic chemicals to increase metabolite production in the dinoflagellate microalga Amphidinium carterae

A new approach to finding optimal centrifugation conditions for shear-sensitive microalgae

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