Data on Wistar Hannover Rats from a General Toxicity Study

Abstract

Abstract:The aim of this study was to collect data on chronological changes in clinical laboratory tests, pathological examinations, and hepatic drug-metabolizing enzymes from Wistar Hannover rats at 8, 10, 19, and 32 weeks of age. The serum triglyceride concentration and the serum LDL cholesterol level were higher in males than in females at all ages. In contrast, serum total protein and creatinine concentrations and cholinesterase activity were lower in males than in females. In addition, sex differences were confirmed in pituitary weight and hepatic CYP3A2 and CYP2C11 activities. In conclusion, the general toxicological data noted in clinical laboratory tests, pathological examinations, and hepatic drug-metabolizing enzymes relating to chronological changes and sex differences may be useful in assessing drug-related toxicity in this strain. Key words: clinical laboratory test, hepatic drug-metabolizing enzyme, pathological examination sprague-dawley (sd) and F344 rats are commonly used for toxicity studies in japan [2], and the inbred F344/N rat substrain has been used for the National toxicity Program rodent toxicity and carcinogenicity bioassays for more than thirty years [10,19]. the F344/N rat is known to have high background incidences of certain types of tumor including testicular interstitial cell tumors and mononuclear cell leukemia [7], while typical spontaneous diseases in aged sd rats are chronic nephropathy and pituitary and mammary tumors [5,16]. therefore, the use of these strains in long-term toxicity studies should be carefully considered when planning study design and assessing toxicity.wistar Hannover rats are used widely in Europe [21], particularly for toxicity studies, because of their small body size, long survival rate, low spontaneous tumor incidence, and ease of handling. research with this strain has been limited in japan; however, its use in toxicity studies is expected to increase. in 2006, Petterino et al. reported clinical chemistry and hematology historical control data from preclinical toxicity studies using sd rats [14], but published historical data on wistar Hannover rats relating to a general toxicity study are scarce [21]. Furthermore, age-related changes and sex differences in serum chemistry values in sd rats have been reported [18]. therefore, collection of general toxico-

A Study for collecting background data on Wistar Hannover [Crl:WI(Han)] rats in general toxicity studies - comparative data to Sprague Dawley rats -

“…Increased relative organ weights of the testes, ovaries and thymus which were considered to be biologically sig-SD rats, absolute weights of heart, kidney and liver were lower in Wistar Han rats than those seen in SD rats and Histopathologic examination revealed increased incidence of corneal mineralization in Wistar Han rats, which is considered to be relevant to the corneal opacities observed during ophthalmology. These lesions were TM :WIST rats and Crl:WI(Han) rats (Hashimoto et al, 2011;Yamatoya et al, 2012). Other histopathologic changes in Wistar Han rats were comparable to those in SD rats and no remarkable changes were observed in macroscopic pathology in either strain of rat.…”

Histological Characteristics of the Regression of Corpora Lutea in Wistar Hannover Rats: the Comparisons with Sprague-Dawley Rats

“…In recent years, Wistar Hannover (WH) rats have become increasingly commonly used for toxicological studies in Japan, and several studies on their suitability for this purpose have been published 6 , 7 , 8 . In our own studies to collect background data for a subchronic (26-week) general toxicity study, we found that the pattern of CL regression differed between WH and Sprague-Dawley (SD [Crl:CD]) (SD) rats.…”

Spontaneously Occurring Formation of Intranuclear and Cytoplasmic Inclusions in Renal Proximal Epithelium Due to Accumulation of d-Amino Acid Oxidase in Wistar Hannover Rats

“…A few years ago, RccHan TM :WIST (Wistar Hannover) rats were introduced to Japan as experimental animals for toxicity testing of pharmaceuticals. However, relatively little background data are available on Wistar Hannover rats (Okamura et al 2011; Yamatoya et al 2012). In a background data study gathered from a 26-week study and carcinogenicity study that used Wistar Hannover rats, we found spontaneous intranuclear and cytoplasmic inclusions in the proximal tubular epithelium.…”