Data report: concentration and carbon isotopic composition in pore fluids from IODP Expedition 385

Torres, Marta E; Kim, Ji Hoon

doi:10.14379/iodp.proc.385.201.2022

Cited by 2 publications

(3 citation statements)

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“…1) which differ in their degree of hydrothermally driven heat flow (Neumann et al, 2023), and in the relative extent of marine vs terrestrial sedimentation (Persad and Masaglia, 2023). Samples yielding PCR amplicons were generally affiliated with subsurface maxima or with broad zones of high alkalinity and DIC concentration (Torres and Kim 2022), a proxy for microbial organic matter remineralization (Supplementary Figures S1 and S2). Sediment samples, DNA yields, and PCR results are summarized in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…1) which differ in their degree of hydrothermally driven heat flow [30], and in the relative extent of marine vs. terrestrial sedimentation [31]. Samples yielding PCR amplicons were generally affiliated with subsurface maxima or with broad zones of high alkalinity and DIC concentration [32], a proxy for microbial organic matter remineralization (Supplementary Figures S1a-h and S2). These geochemical layers often coincide with methane-sulfate transition zones, which are shown to have elevated microbial cell counts and increased microbial activity [33].…”

Section: Resultsmentioning

confidence: 99%

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PCR-based survey of methane-cycling archaea in methane-soaked subsurface sediments of Guaymas Basin, Gulf of California

Hinkle,

Mara,

Beaudoin

et al. 2023

Preprint

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The Guaymas Basin in the Gulf of California is characterized by active seafloor spreading, rapid deposition of organic-rich sediments, steep geothermal gradients, and abundant methane of mixed thermogenic and microbial origin. Subsurface sediment samples were selected from eight drilling sites to explore the diversity, depth range, and in-situ temperature range of methane-cycling archaea in the Guaymas Basin subsurface, using PCR amplification with general (mcrIRD) and ANME-1 specific primers targeting the alpha (α) subunit of methyl coenzyme M reductase, encoded by themcrAgene. Diverse ANME-1 lineages were detected in seven drilling sites, preferentially around the methane-sulfate interface, and in several cases showed preferences for specific sites and types of sediment. Phylogenetically, most ANME-1 sequences from the Guaymas Basin subsurface were related to similar sequences from marine mud volcanoes, seep sites, and the shallow marine subsurface. Methanogenic lineages were found in three sites. The most frequently recovered phylotypes, closely affiliated with the hyperthermophilicMethanocaldococcaceae, were found at the hydrothermally influenced Ringvent site. The coolest drilling site, in the northern axial trough of Guaymas Basin, yielded the greatest diversity of methanogen lineages. Our survey indicates extensive microbial methane cycling within subsurface sediments of Guaymas Basin, and calls attention to deep sources of thermogenic methane where potential microbial processing remains to be shown.ImportanceThe Guaymas Basin has received significant attention from microbiologists and biogeochemists due to the abundance of methane in its sediments. This study has identified new ANME-1 and methanogenic lineages and phylogenetic clusters present in subsurface Guaymas Basin sediments. Our work allows for a greater understanding of 1) the diversity of methane-cycling archaea and 2) methane-cycling in the Guaymas Basin.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

PCR-based survey of methane-cycling archaea in methane-soaked subsurface sediments of Guaymas Basin, Gulf of California

Hinkle,

Mara,

Beaudoin

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…PCR-amplified mcrA genes were obtained from nearly all drilling sites in the IODP Expedition 385 (Figure 1) which differ in their degree of hydrothermally driven heat flow [30], and in the relative extent of marine vs. terrestrial sedimentation [31]. Samples yielding PCR amplicons were generally affiliated with subsurface maxima or with broad zones of high alkalinity and DIC concentration [32], a proxy for microbial organic matter remineralization (Supplementary Figures S1a-h and S2). These geochemical layers often coincide with methane-sulfate transition zones (SMTZs), which are shown to have elevated microbial cell counts and increased microbial activity [33].…”

Section: Resultsmentioning

confidence: 99%

A PCR-Based Survey of Methane-Cycling Archaea in Methane-Soaked Subsurface Sediments of Guaymas Basin, Gulf of California

Hinkle,

Mara,

Beaudoin

et al. 2023

Microorganisms

View full text Add to dashboard Cite

The Guaymas Basin in the Gulf of California is characterized by active seafloor spreading, the rapid deposition of organic-rich sediments, steep geothermal gradients, and abundant methane of mixed thermogenic and microbial origin. Subsurface sediment samples from eight drilling sites with distinct geochemical and thermal profiles were selected for DNA extraction and PCR amplification to explore the diversity of methane-cycling archaea in the Guaymas Basin subsurface. We performed PCR amplifications with general (mcrIRD), and ANME-1 specific primers that target the alpha (α) subunit of methyl coenzyme M reductase (mcrA). Diverse ANME-1 lineages associated with anaerobic methane oxidation were detected in seven out of the eight drilling sites, preferentially around the methane-sulfate interface, and in several cases, showed preferences for specific sampling sites. Phylogenetically, most ANME-1 sequences from the Guaymas Basin subsurface were related to marine mud volcanoes, seep sites, and the shallow marine subsurface. The most frequently recovered methanogenic phylotypes were closely affiliated with the hyperthermophilic Methanocaldococcaceae, and found at the hydrothermally influenced Ringvent site. The coolest drilling site, in the northern axial trough of Guaymas Basin, yielded the greatest diversity in methanogen lineages. Our survey indicates the potential for extensive microbial methane cycling within subsurface sediments of Guaymas Basin.

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Data report: concentration and carbon isotopic composition in pore fluids from IODP Expedition 385

Abstract: Introduction 2 Study sites 3 Analytical methods 4 Results 6 Acknowledgments 7 References

Cited by 2 publications

References 36 publications

PCR-based survey of methane-cycling archaea in methane-soaked subsurface sediments of Guaymas Basin, Gulf of California

PCR-based survey of methane-cycling archaea in methane-soaked subsurface sediments of Guaymas Basin, Gulf of California

A PCR-Based Survey of Methane-Cycling Archaea in Methane-Soaked Subsurface Sediments of Guaymas Basin, Gulf of California

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