Dataset from a proteomics analysis of tumor antigens shared between an allogenic tumor cell lysate vaccine and pancreatic tumor tissue.

Stingl, Christoph; Lau, Sai Ping; Burg, Sjoerd H. van der; Aerts, Joachim; Eijck, Casper H.J. van; Luider, Theo M.

doi:10.1016/j.dib.2022.108490

Cited by 2 publications

(2 citation statements)

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“…The creation of a comparable database was carried out with tandem mass tags followed by prefractionation with high‐pH RPLC into 24 fractions. The database contained peptides sequence, charge, and length as a function of each fraction and could identify 61 primary tumor antigens [136]. Prostate cancer was also studied with a targeted proteome strategy, with the generation of a prostate cancer tissue‐specific peptide library for posterior comparison with digested clinical prostate samples [137].…”

Section: High‐ph Rplcmentioning

confidence: 99%

“…With similar fractionation strategies, bottom-up studies employed offline high-pH RPLC to screen digested peptides from a variety of biological samples. Between these samples are pancreas cancer tissue [136], prostate cancer tissue [137], bronchoalveolar lavage fluid for lung can-cer biomarker research [138], Trypanosoma brucei cells [139], primary biliary cholangitis from human serum [140], Panax ginseng cauline leaf and root [141], Saccharomyces cerevisiae yeast [142], and HeLa cells [143][144][145].…”

Section: Offline High-ph Rplc Approachesmentioning

confidence: 99%

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Recent stationary phase‐based fractionation strategies in proteomic analysis

Ribeiro

Urban

2023

J of Separation Science

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In this article, we review stationary phase‐assisted sample prefractionation in proteomic analysis in 2019–2022 period. Applications are grouped according to the mode of retention used in the prefractionation step prior to the final analysis by low‐pH reversed‐phase liquid chromatography‐tandem mass spectrometry. Online or offline instrumental configurations are also commented on, with a highlight on novel online platforms. From the articles reviewed in this period, the order of popularity of chromatographic modes for sample fractionation is affinity chromatography > size exclusion chromatography > hydrophilic interaction chromatography > high‐pH reversed‐phase liquid chromatography > ion exchange chromatography.

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Section: High‐ph Rplcmentioning

confidence: 99%