Dataset on 21 autosomal and two sex determining short tandem repeat loci in the Kedayan population in Borneo, Malaysia

Hakim, Hashom Mohd; Khan, Hussein Omar; Ismail, Siti Afifah; Lalung, Japareng; Kofi, Abban Edward; Abdullah, Mohd Tajuddin; Chambers, Geoffrey K.; Edinur, Hisham Atan

doi:10.1016/j.dib.2020.105909

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…The STR population datasets currently available for various population groups in Malaysia are listed in Table 3 . These datasets were mainly produced by researchers from academic institutions [ 8 , 9 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 ], and are not part of the RMP database. Mathematical probabilities calculated from these data do indicate that the STR loci tested are reliable for the identification of individuals in criminal cases and for kinship analysis associated with paternity disputes and immigration applications.…”

Section: Dna Population Data For Casework and Paternity Testing Stmentioning

confidence: 99%

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Hakim

Khan²,

Lalung

et al. 2020

Genes

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Science and technology are extensively used in criminal investigation. From the mid- to late-1980s, one of the scientific discoveries that has had a particularly remarkable impact on this field has been the use of highly variable DNA sequence regions (minisatellites) in the human genome for individual identification. The technique was initially referred to as DNA fingerprinting, but is now more widely referred to as DNA profiling. Since then, many new developments have occurred within this area of science. These include the introduction of new genetic markers (microsatellites also known as short tandem repeats/STRs), the use of the polymerase chain reaction for target amplification, the development of DNA databases (databanking), and the advancement and/or improvement of genotyping protocols and technologies. In 2019, we described the progress of DNA profiling and DNA databanking in Malaysia for the first time. This report included information on DNA analysis regulations and legislation, STR genotyping protocols, database management, and accreditation status. Here, we provide an update on the performance of our DNA databank (numbers of DNA profiles and hits) plus the technical issues associated with correctly assigning the weight of evidence for DNA profiles in an ethnically diverse population, and the potential application of rapid DNA testing in the country. A total of 116,534 DNA profiles were obtained and stored in the Forensic DNA Databank of Malaysia (FDDM) by 2019, having increased from 70,570 in 2017. The number of hits increased by more than three-fold in just two years, where 17 and 69 hits between the DNA profiles stored in the FDDM and those from crime scenes, suspects, detainees, drug users, convicts, missing persons, or volunteers were recorded in 2017 and 2019, respectively. Forensic DNA analysis and databanking are thus progressing well in Malaysia and have already contributed to many criminal investigations. However, several other issues are discussed here, including the need for STR population data for uncharacterized population groups, and pilot trials for adopting rapid DNA profiling technology. These aspects should be considered by policy makers and law enforcement agencies in order to increase the reliability and efficiency of DNA profiling in criminal cases and in kinship analysis in Malaysia.

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Section: Dna Population Data For Casework and Paternity Testing Stmentioning

confidence: 99%

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Hakim

Khan²,

Lalung

et al. 2020

Genes

Self Cite

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Evaluation of long-term storage effects on buccal cell DNA from untreated cards for STR profiling

Hakim

Lalung

Khan³

et al. 2020

IOP Conf. Ser.: Earth Environ. Sci.

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The success of DNA profiling using long-term stored samples depends on the amount and quality of their recovered DNA templates. Physical and biochemical factors such as microbial activity, humidity and temperature contribute to DNA degradation. In this study, we used genomic DNA extracted from buccal cell samples that were stored for more than 4 years on Bode Buccal DNA Collector™ cards (Bode Technology, Virginia, USA) for typing 27 and 24 short tandem repeat (STR) loci using Powerplex® Fusion 6C system (Promega Corporation, Madison, USA) and Globalfiler™ Express kit (Thermo Fisher Scientific, USA), respectively. Our results demonstrated that the Buccal DNA Collector can be used as a collection medium for buccal swab samples that are not immediately analyzed or those that need to be retrospectively analyzed. There is a sign of DNA degradation which might well be expected because buccal cell samples were deposited on untreated filter paper and have been stored for a long period (> four years) at room temperature. However, STR allele calls were obtained from most of the buccal cell samples, especially when typed using the Powerplex® Fusion 6C system kit.

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Dataset on 21 autosomal and two sex determining short tandem repeat loci in the Kedayan population in Borneo, Malaysia

Cited by 2 publications

References 12 publications

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Autosomal STR Profiling and Databanking in Malaysia: Current Status and Future Prospects

Evaluation of long-term storage effects on buccal cell DNA from untreated cards for STR profiling

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