Dataset on the effect of flavonoids on the stabilization of the resin-dentin interface

Abstract

Data in this article are associated with our research article “Effect of Myricetin on Odontoblast-like Cells and its Potential to Preserve Resin–Dentin Bonds.” Both a poor infiltration of resin monomers into the demineralized dentin matrix and hydrolytic degradation of the adhesive could lead to the instability of the resin–dentin interface. The degradation of collagen is caused by matrix metalloproteinases (MMP) and cysteine cathepsins. These collagenolytic enzymes are contained in their latent form as pro-MMPs in the dentinal structure, and undergo activation during the adhesive process. Given that the integrity of the collagen matrix is essential for the preservation of the dentin bond strength in both the medium and long term, the inhibition of these proteases is necessary to improve the durability of adhesive restorations. Among the different strategies suggested to improve both the behavior of the substrate against enzymatic degradation and the biomechanical behavior of the adhesive interface, the use of protease inhibitors and collagen crosslinking agents has been recommended, such as polyphenols. Research has focused on flavonoids such as proanthocyanidins (PAC), a class of phenolic compounds found in a variety of plants such as blueberry and grape whose chemical structure favors their action as cross-linking agents. However, the focus has recently shifted towards myricetin (MYR) due to its chemical structure: a greater amount of hydroxyl groups at the substitution positions, which form bonds with the carbonyl groups of the side chains of collagen amino acids and generate interfiber bonds. Our previous study has shown the efficacy of MYR both as a cross-linking agent and as a MMP inhibitor without any immediate effects on microtensile bond strength (µTBS) and preserving it for six months after storage, and maintaining the odontoblastic phenotype without affecting cell viability. The objective of this article is to present a dataset on the effect of flavonoids PAC and MYR on the resin–dentin interface. Given that durability of the resin – dentin bond holds great importance for the clinical longevity of adhesive restorations, our data aims to show the effects of these flavonoids on resin – dentin µTBS after 18-month storage. Test groups for the µTBS assay were set as follows: G1 (negative control), conventional adhesion technique; G2 (vehicle control), 100% ethanol (EtOH) for 120 s; G3, 0.2% chlorhexidine (CHX) for 60 s; G4, 1% glutaraldehyde (GA) for 60 s; and G5, 600 µM myricetin (MYR) for 120 s. Datasets were exported to SPSS software, version 21.0 (SPSS, Chicago, IL, USA) for analysis using the Shapiro–Wilk, a two-way analysis of variance including factor interactions (treatment and storage time). Data are presented as mean ± standard deviation (SD). Differences with p-values < 0.05 were considered significant. Our data can be used as a basis for comparison among other natural and synthetic substances that could work as MMP inhibitors and crosslinking ...

Preliminary Evaluation of Bioactive Collagen–Polyphenol Surface Nanolayers on Titanium Implants: An X-ray Photoelectron Spectroscopy and Bone Implant Study

“…Indeed, the crosslinking of collagen by polyphenols has been widely investigated and applied in many technological domains [32,38]. To stay in medical applications, polyphenolic compounds and mixtures are used to cross-link the collagen of dentine to increase the mechanical strength of the adhesion layer between dentin and composites [42][43][44][45][46][47]. Proanthocyanidins (PA), which make up a significant fraction of the extract we used, have been shown to induce effects both to the secondary and tertiary structure of collagen, and a significant role for hydrogen bonding in the stabilization of collagen by PA has been suggested [48][49][50].…”

The Effect of Two Nano Proanthocyanidin-Based Dentin Biomodifiers on Micro Tensile Bond Strength of Dentin Treated with Two Adhesive Modes

“…This could explain the highest values recorded in groups using the total-etch mode, disregarding the dentine pre-treatment agents used. On the other hand, stable bonds can only be achieved if the adhesive infiltrates uniformly the etched dentine in order to prevent the different degrees of incomplete infiltration (20) that, if happens, a trapped demineralized zone at the bottom of the hybrid layer (HL) will occur (21) .…”

Uso do extrato de romã (Punica granatum L.) na superfície dentinária erodida: ação antioxidante, resistência de união e análise morfológica da interface adesiva após envelhecimento

“…Acredita-se que os flavonóides presentes nos extratos naturais sejam capazes de induzir ligações moleculares de colágeno e melhorar as propriedades mecânicas da dentina, bem como funcionar como inibidores de MMPs e impedir a degradação precoce da interface adesiva (Baldion & Betancourt, 2021;Paik et al, 2022). Além disso, os compostos naturais contendo bioativos melhoram a molhabilidade do substrato dental e deixam a superfície quimicamente preparada para os procedimentos adesivos (Ururahy et al, 2017).…”