“…Daun02 is catalyzed by βgal into daunorubicin, a toxic compound that leads to early inactivation of calcium-dependent action potentials (Engeln et al, 2016), followed by apoptosis and cell death only in the strongly activated βgal-expressing neurons (Pfarr et al, 2015). After a couple of days, the behavioral effects of the targeted neural inactivation by Daun02 can be assessed under a drug free condition (Bossert et al, 2011; Fanous et al, 2012; Koya et al, 2016) to demonstrate causal roles for neuronal ensembles within multiple nodes of the corticostriatal circuit, including prefrontal cortex, striatum and amygdala (Bossert et al, 2011; Cruz et al, 2013, 2014, 2015; Pfarr et al, 2015; de Guglielmo et al, 2016; Funk et al, 2016; Koya et al, 2016; Suto et al, 2016; Caprioli et al, 2017). …”