Day 4 versus day 3 embryo transfer: a prospective study of clinical outcomes

Pantos, Konstantinos; Makrakis, Evangelos; Chronopoulou, Margaret; Biba, Maria; Perdikaris, Anastasios; Dafereras, Alex

doi:10.1016/j.fertnstert.2007.03.056

Cited by 22 publications

(17 citation statements)

References 26 publications

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“…Current literature evaluating day 4 transfers describes advantages compared to day 2 or 3 transfers [38–41] and no significant difference between day 4 and day 5 transfers with regard to single embryo transfers (SET) [3]. The largest prospective study comparing culture durations of 3 to 5 days in a single-step medium showed comparable pregnancy rates in all groups [9] with pregnancy rates of 25.8% after day 4 transfers ( n = 475) and 27.8% after day 5 transfers ( n = 694).…”

Section: Discussionmentioning

confidence: 99%

Pregnancy rates of day 4 and day 5 embryos after culture in an integrated time-lapse incubator

Holschbach

Weigert

Dietrich

et al. 2017

Reprod Biol Endocrinol

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BackgroundThe aim of this study was to compare pregnancy rates in patients undergoing IVF/ICSI with embryo transfer after 4 and 5 days of culture in a closed incubation system with integrated time-lapse imaging.MethodsOut of n = 2207 in vitro fertilization (IVF)/ intracytoplasmic sperm injection (ICSI) cycles performed between January 2011 and April 2016 at a tertiary referral university hospital, a total of n = 599 IVF/ICSI cycles with prolonged embryo culture in an integrated time-lapse system (EmbryoScope© (Vitrolife)) until day 4 or 5 were retrospectively analyzed with regard to embryo morphology and pregnancy rates.ResultsA transfer on day 5 compared to a transfer on day 4 did not result in higher implantation and clinical pregnancy rates (IR 29.4% on day 4 versus 33.0% on day 5, p = 0.310; CPR 45.2% on day 4 versus 45.7% on day 5, p = 1.0). The percentage of ideal embryos transferred on day 4 was comparable to the rate of ideal embryos transferred on day 5 (41.6% versus 44.1%, p = 0.508). However, on day 4 a significantly higher number of embryos was transferred (1.92 on day 4 versus 1.84 on day 5, p = 0.023), which did not result in higher rates of multiple pregnancies.ConclusionsPregnancy rates in IVF/ICSI cycles with integrated time-lapse incubation and transfer on day 4 and 5 are comparable. This finding provides the clinician, IVF laboratory and patient with more flexibility.Trial registrationThis study was retrospectively registered by the local ethics committee of the University of Heidelberg on December 19, 2016 (registration number S-649/2016).

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Section: Discussionmentioning

confidence: 99%

Pregnancy rates of day 4 and day 5 embryos after culture in an integrated time-lapse incubator

Holschbach

Weigert

Dietrich

et al. 2017

Reprod Biol Endocrinol

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“…After fertilization, the embryo usually consists of four blastomeres at 48 h, and an average of eight cells at 72 h. To date, no study has demonstrated the superiority of transfer on day 3 versus day 2 [1,2,20,32,34]. With the current criteria, an extra day at this stage does not provide enough additional information concerning the developmental potential of the embryo [27,36].…”

Section: Introductionmentioning

confidence: 97%

Early compaction at day 3 may be a useful additional criterion for embryo transfer

Cruguel¹,

Ferré-L’Hôtellier²,

Morinière³

et al. 2013

J Assist Reprod Genet

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“…It seems that the transfer of day 4 embryos has rarely been attempted by ART specialists even if such a transfer with embryos that have undergone compaction and reached the morula stage may have certain advantages [20]. …”

Section: Discussionmentioning

confidence: 99%

Comparison of the clinical outcomes of day 4 and 5 embryo transfer cycles

Lee

Lim

et al. 2013

Clin Exp Reprod Med

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ObjectiveThe majority of embryo transfers (ETs) to date have been performed on day 3 to reduce the potential risk of developmental arrest of in vitro cultured embryos before ET. Development of sequential media has significantly improved culture conditions and allowed blastocyst transfer on day 5. While day 5 ET provides higher clinical pregnancy outcomes with reduced risks of multiple pregnancies, it still has potential risks of developmental arrest of IVF embryos. The aim of this study was to evaluate the clinical outcomes of day 4 ETs and compare the efficacy of day 4 ET with day 5 ET.MethodsFrom 2006 to 2009, a total of 747 fresh IVF-ET cycles were retrospectively analyzed (day 4, n=440 or and day 5, n=307). The cycles with any genetic factors were excluded. The rates of matured oocytes, fertilization, good embryos, and clinical pregnancy of the two groups were compared. The chi-square test and t-test were used for statistical analysis.ResultsThere were no significant differences between the two groups with respect to the mean age of the females and rates of matured oocytes. The pregnancy outcomes of day 4 ET (40.7%) were similar to those of day 5 ET (44.6%). The implantation rate of day 5 ET (24.2%) was significantly higher than that of day 4 ET (18.4%) (p=0.003).ConclusionDay 4 ET can be chosen to avoid ET cancellation in day 5 ET resulting from suboptimal circumstances in the IVF laboratory, but the decremented quality of embryos for transfer and the decreased pregnancy rate must be taken into consideration.

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Day 4 versus day 3 embryo transfer: a prospective study of clinical outcomes

Cited by 22 publications

References 26 publications

Pregnancy rates of day 4 and day 5 embryos after culture in an integrated time-lapse incubator

Pregnancy rates of day 4 and day 5 embryos after culture in an integrated time-lapse incubator

Early compaction at day 3 may be a useful additional criterion for embryo transfer

Comparison of the clinical outcomes of day 4 and 5 embryo transfer cycles

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