“…The causes of delayed first AI after calving in the present study were not fully understood and it is necessary to find out the factors contributed in. Previous studies reported that the causes of delayed first AI after calving in dairy cows include management and the cow factors (Yusuf et al, 2011), in which could be break down to the factors such as extended voluntary waiting period (Arbel et al, 2001), infection of the reproductive tract (Dohoo, 1983;Gilbert et al, 2005;Yusuf et al, 2011), season of calving, herd and parity (Darwash et al, 1997;Yusuf et al, 2011), abnormal resumption of ovarian cycle (Lamming & Darwash, 1998;Yusuf et al, 2006;Yusuf et al, 2011), and negative energy balance (Lucy, 2001;Wiltbank et al, 2006). In this study, all cows were housed using tie-stall.…”