DDX6 Helicase Behavior and Protein Partners in Human Adipose Tissue-Derived Stem Cells during Early Adipogenesis and Osteogenesis

Abstract

DDX6 helicase is an RNA-binding protein involved in different aspects of gene expression regulation. The roles played by DDX6 depend on the complexes associated with it. Here, for the first time, we characterize the protein complexes associated with DDX6 in human adipose tissue-derived stem cells (hASCs) and analyze the dynamics of this helicase under different conditions of translational activity and differentiation. The results obtained demonstrated that the DDX6 helicase is associated with proteins involved in the control of mRNA localization, translation and metabolism in hASCs. DDX6 complexes may also assemble into more complex structures, such as RNA-dependent granules, the abundance and composition of which change upon inhibited translational activity. This finding supports the supposition that DDX6 is possibly involved in the regulation of the mRNA life cycle in hASCs. Although there was no significant variation in the protein composition of these complexes during early adipogenic or osteogenic induction, there was a change in the distribution pattern of DDX6: the number of DDX6 granules per cell was reduced during adipogenesis and was enhanced during osteogenesis.

Human Amniotic Epithelial Cells as a Tool to Investigate the Effects of Cyanidin 3-O-Glucoside on Cell Differentiation

“…A downregulated gene, DDX6 , which encodes DEAD-box helicase 6 protein, has been reported to function in adipogenesis. In human adipose tissue-derived stem cells, the number of DDX6 granules per cell was reduced during adipogenesis [ 42 ].…”

3D Poly(Lactic Acid) Scaffolds Promote Different Behaviors on Endothelial Progenitors and Adipose-Derived Stromal Cells in Comparison With Standard 2D Cultures

“…hASCs were isolated from adipose tissue obtained from one otherwise healthy female donor (BMI = 27.03) who had undergone liposuction surgery. The isolation protocol followed previously published procedures ( Rebelatto et al, 2008 ; Marcon et al, 2020 ). In short, the tissue was first washed with sterile phosphate-buffered saline (PBS) and then digested with collagenase type I (Gibco ® , Invitrogen ® , Carlsbad, CA, United States) for 30 min at 37°C, 5% CO 2 and under constant agitation.…”

Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins

“…NBDY (non-annotated P-body dissociating polypeptide) was initially characterized as a human microprotein component of the mRNA decapping complex, directly interacting with EDC4 and DCP1A being localized in Pbodies' granules [53,57]. It has recently been demonstrated that DCP1A is dispersed in granular structures in the hASC cytoplasm, partially co-located with DDX6 (another interaction partner of NBDY) [53,58]. Another example is the smORF of LINC00116 that encodes the 56-aa microprotein MTLN (Figure 3B).…”