De novo adventitious root formations in mini-cuttings of Azadirachta indica in response to different rooting media and auxin treatments

Gehlot, Ashok; Gupta, Reena; Arya, I. D.; Arya, Sarita; Tripathi, Atul

doi:10.3832/ifor1189-007

Cited by 18 publications

(21 citation statements)

References 15 publications

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“…On the basis of our results, and regardless of the application method, rooting percentage generally increased with increasing IBA concentration (Table 1). Similarly, a strong relationship has been observed between the concentration of auxin and rooting frequency in cuttings of Azadiratcha indica (Gehlot et al 2014). However, the auxin application method is also important because it affected the rooting response and the quality of the rooted plantlets.…”

Section: Discussionmentioning

confidence: 94%

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Vielba

Vidal

Ricci

et al. 2020

Israel J. Plant Sci.

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The present study investigated how auxin concentration and the method of application affected the formation of adventitious roots in microshoots of chestnut (Castanea sativa) and oak (Quercus robur). The activity of two urea derivatives (2, 3-MDPU and 3, 4-MDPU) was also evaluated. Microshoots were derived from basal sprouts of two mature chestnut trees (P1 and P2) and one adult oak genotype (Sainza). In chestnut, rooting percentage was positively affected by auxin in a dose-dependent manner, particularly in shoots treated with the hormone for 24 h. The effect of auxin on rooting also differed depending on the application method. In shoots treated for 24 h, the highest concentration of auxin produced the healthiest rooted plantlets, in terms of the root system and shoot quality. By contrast, in shoots treated by the basal quick-dip method, the shoot quality was best at the lowest auxin concentration. The effect of urea derivatives on the root system depended on the species as well as on the auxin concentration and application period. Use of the MDPUs improved the root system architecture of auxin-treated shoots by promoting lateral root development and triggering the synchronous initiation of root primordia at the base of the shoot. Shoot quality was also improved by MDPUs, which promoted resumption of growth and reduced shoot-tip necrosis.

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Section: Discussionmentioning

confidence: 94%

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Vielba

Vidal

Ricci

et al. 2020

Israel J. Plant Sci.

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“…LIKE AUXIN RESISTANT ( LAX ) and AUXIN RESISTANT ( AUX s) act as auxin transports; only Potri.004G172800 (best hit to AT2G21050.1 , LAX2 ) shows an upregulated expression pattern during AR formation ( Swarup and Bhosale, 2019 ; An et al, 2020 ; Li, 2021 ). The expression level of Potri.008G161200 (best hit of AT4G14550.1 , IAA14 ) was increased up to 4.9, 20.9, 24.2, 50.7, and 30.4 times on different days, which was a member of the Aux/IAA protein family and involved in lateral root development ( Gehlot et al, 2015 ; Yang et al, 2019 ). From 0 to 10 d, the expression of Potri.011G042400 (best hit to AT3G62980 , TIR1) increased continuously, which may be located upstream of the auxin regulatory pathway ( Shu et al, 2019 ).…”

Section: Resultsmentioning

confidence: 99%

Transcriptomic profiles of poplar (Populus simonii × P. nigra) cuttings during adventitious root formation

Meng²,

Chen³

et al. 2022

Front. Genet.

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The formation of adventitious roots (ARs) is vital for the vegetative propagation of poplars. However, the relevant mechanisms remain unclear. To reveal the underlying molecular mechanism, we used RNA-seq to investigate the transcriptional alterations of poplar cuttings soaked in water for 0, 2, 4, 6, 8, and 10 d; 3,798 genes were differentially expressed at all the time points, including 2,448 upregulated and 1,350 downregulated genes. Biological processes including “cell cycle,” “photosynthesis,” “regulation of hormone levels,” and “auxin transport” were enriched in the differentially expressed genes (DEGs). KEGG results showed that the common DEGs were most enriched in the pathway of “Carbon fixation in photosynthetic organisms” and “Starch and sucrose metabolism.” We further dissected 38 DEGs related to root and auxin, including two lateral root primordium 1 (LRP1), one root meristem growth factor (RGF9), one auxin-induced in the root (AIR12), three rooting-associated genes (AUR1 and AUR3), eight auxin transcription factors (ARFs and LBDs), 10 auxin respective genes (SAURs and GH3s), nine auxin transporters (PINs, ABCs, LAX2, and AUXs), and four auxin signal genes (IAAs and TIR1). We found that the rooting abilities of poplar cuttings with and without leaves are different. By applying different concentrations of IBA and sucrose to the top of cuttings without leaves, we found that 0.2 mg/ml IBA and 2 mg/ml sucrose had the best effect on promoting AR formation. The transcriptome results indicated photosynthesis may influence AR formation in poplar cuttings with leaves and revealed a potential regulatory mechanism of leafy cuttage from poplar cuttings. In addition, we provided a new perspective to resolve rooting difficulties in recalcitrant species.

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“…Cannabis cuttings in this experiment were exposed to a 100 mg L −1 IBA solution following the "long basal soak" protocol for herbaceous cuttings by Kroin [37], which provided guidelines for hormone solution concentrations and several ways of exposing plant material. This method was also employed by Gehlot [38] on vegetative propagation of neem (Azadirachta indica), although the actual exposure time was not listed. Nevertheless, across a range of hormone solution concentrations (250; 500; 750; 1000; 1500 mg L −1 ), 250 mg L −1 IBA provided the best results, e.g., the highest rooting success (80%) and highest number of roots (71), compared to other hormone concentrations [38].…”

Section: Discussionmentioning

confidence: 99%

“…This method was also employed by Gehlot [38] on vegetative propagation of neem (Azadirachta indica), although the actual exposure time was not listed. Nevertheless, across a range of hormone solution concentrations (250; 500; 750; 1000; 1500 mg L −1 ), 250 mg L −1 IBA provided the best results, e.g., the highest rooting success (80%) and highest number of roots (71), compared to other hormone concentrations [38]. In the current study, Kroin's [37] "long basal soak" method was chosen over more time-efficient methods because it was suspected that bacterial infection of cuttings would be less likely to occur in seconds or minutes of inoculation.…”

Section: Discussionmentioning

confidence: 99%

Alternative Rooting Methods for Medicinal Cannabis Cultivation in Denmark—Preliminary Results

Favero,

Salomonsen,

Lütken

2023

Plants

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Cannabis sativa L. is typically propagated through micropropagation or vegetative cuttings, but the use of root-inducing hormones, such as indole-3-butyric acid (IBA), is not allowed for growing medicinal cannabis in Denmark. This study examined alternative rooting treatments, including Rhizobium rhizogenes inoculation, water-only as well as IBA treatments, in eight cannabis cultivars. PCR on root tissue suggested that 19% of R. rhizogenes-inoculated cuttings were transformed. These were derived from “Herijuana”, “Wild Thailand”, “Motherlode Kush”, and “Bruce Banner”, indicating a variation in cultivar susceptibility toward R. rhizogenes. A 100% rooting success was achieved regardless of cultivar and treatment, suggesting that alternative rooting agents are not required for efficient vegetative propagation. However, rooted cuttings differed in shoot morphology with improved shoot growth in cuttings treated with R. rhizogenes (195 ± 7 mm) or water (185 ± 7 mm) while inhibited shoot growth under IBA treatment (123 ± 6 mm). This could have advantageous economic implications should cuttings not treated with hormone reach maturity faster than those exposed to the hormone, thereby contributing to completing a full growing cycle more effectively. IBA exposure increased root length, root dry weight, and root/shoot dry weight ratio compared to cuttings treated with R. rhizogenes or water but simultaneously inhibited shoot growth compared to these.

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De novo adventitious root formations in mini-cuttings of Azadirachta indica in response to different rooting media and auxin treatments

Cited by 18 publications

References 15 publications

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Effects of auxin and urea derivatives on adventitious rooting in chestnut and oak microshoots

Transcriptomic profiles of poplar (Populus simonii × P. nigra) cuttings during adventitious root formation

Alternative Rooting Methods for Medicinal Cannabis Cultivation in Denmark—Preliminary Results

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