De novo assembly and analysis of midgut transcriptome of Haemaphysalis flava and identification of genes involved in blood digestion, feeding and defending from pathogens

Xu, Xing-Li; Cheng, Tao; Yang, Huanming; Liao, Zhihui

doi:10.1016/j.meegid.2015.12.005

Cited by 34 publications

(43 citation statements)

References 68 publications

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“…In the gut transcriptome of R. microplus, binding and catalytic activity was also identified as the most common functional types of activity (HEEKIN et al, 2013). The same results were observed by Xu et al (2016), who classified 47 and 42% of the transcripts of H. flava in the functional categories binding and catalytic activity, respectively. In the proteomic study of gut of O. erraticus, Oleaga et al (2015) also identified catalytic activity and binding as the most prevalent activities within their set of proteins, in accordance to our results.…”

Section: Discussionsupporting

confidence: 68%

“…Thus, knowledge about the vital molecular constituents of ticks, such as genes and/or proteins, have become the target of several studies (CASTRO et al, 2016;CHMELAŘ et al, 2016;DÍAZ-MARTÍN et al, 2015;HEEKIN et al, 2013;KOTSYFAKIS et al, 2015;PERNER et al, 2016;RIBEIRO et al, 2017;XU et al, 2016). These studies have sought to ascertain and understand the roles of the molecules involved in the tick-host or tick-pathogen relationship.…”

Section: Introductionmentioning

confidence: 99%

“…DNA sequencing technologies, together with bioinformatics tools, allow a broad view of the probable protein constituents of a tissue, by isolating the messenger RNA repertoire and sequencing of the cDNA (complementary DNA), leading to an increasing knowledge of the putative molecules expressed by a tissue, such as, salivary gland and midgut, from different species (CASTRO et al, 2016;CHMELAŘ et al, 2016;KOTSYFAKIS et al, 2015;PERNER et al, 2016;RIBEIRO et al 2017;SCHWARZ et al, 2014;VALENZUELA, 2004;XU et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Peptidases such as cathepsins, legumain, aspartic, metallo and serine proteinase are cited as the main proteolytic enzymes actives in the digestive machinery of hemoglobin (SOJKA et al, 2013). All these peptidases have been identified and characterized by biochemical, proteomic and transcriptomic studies in different tick species, such as Dermacentor variabilis (Say, 1821) (ANDERSON et al, 2008), Rhipicephalus microplus (Canestrini, 1888) (MENDIOLA et al, 1996;KONGSUWAN et al, 2010;HEEKIN et al, 2013), Ixodes ricinus Linneaus, 1758 (SOJKA et al, 2007(SOJKA et al, , 2008(SOJKA et al, , 2013HORN et al, 2009;FRANTA et al, 2010;KOTSYFAKIS et al, 2015;PERNER et al, 2016), Haemaphysalis longicornis Neumann, 1901(KOH et al, 1990BOLDBAATAR et al, 2006), Haemaphysalis flava Neumann 1897 (XU et al, 2016), Ornithodoros tholozani (Laboulbène and Mégnin, 1882) (AKOV et al, 1976), Ornithodoros moubata (Murray, 1877) (GRANDJEAN, 1984) and Ornithodoros erraticus (Lucas, 1849) (OLEAGA et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, midgut transcriptome analysis of nymphs and adult of I. ricinus revealed several enzymes associated with protein, carbohydrate and lipid digestion; transporters and channels that might be associated with nutrient uptake; and immunity-related transcripts including antimicrobial peptides (KOTSYFAKIS et al, 2015). Xu et al (2016) analyzed the midgut transcriptome of H. flava and identified transcripts associated with metabolic processes, suggesting that they were involved in the blood meal digestion, such as peptidases, inhibitors peptidases, phospholipase, hydrolases and immunity-related proteins.…”

Section: Introductionmentioning

confidence: 99%

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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Ornithodoros mimon is an argasid tick that parasitizes bats, birds and opossums and is also harmful to humans. Knowledge of the transcripts present in the tick gut helps in understanding the role of vital molecules in the digestion process and parasite-host relationship, while also providing information about the evolution of arthropod hematophagy. Thus, the present study aimed to know and ascertain the main molecules expressed in the gut of argasid after their blood meal, through analysis on the gut transcriptome of engorged females of O. mimon using 454-based RNA sequencing. The gut transcriptome analysis reveals several transcripts associated with hemoglobin digestion, such as serine, cysteine, aspartic proteases and metalloenzymes. The phylogenetic analysis on the peptidases confirmed that most of them are clustered with other tick genes. We recorded the presence a cathepsin O peptidase-coding transcript in ticks. The topology of the phylogenetic inferences, based on transcripts of inferred families of homologues, was similar to that of previous reports based on mitochondrial genome and nuclear rRNA sequences. We deposited 2,213 sequence of O. mimon to the public databases. Our findings may help towards better understanding of important argasid metabolic processes, such as digestion, nutrition and immunity.Keywords: Argasidae, transcriptome, gut, phylogeny, Ornithodoros mimon. ResumoOrnithodoros mimon é um carrapato argasídeo parasita de morcegos, aves e marsupiais, além de ser bastante agressivo aos humanos. O conhecimento dos transcritos presentes no intestino dos carrapatos auxilia no entendimento do papel de moléculas vitais no processo de digestão e na relação parasito-hospedeiro, além de fornecer também informações sobre a evolução dos artrópodes hematófagos. Desta maneira, o presente estudo teve como objetivo conhecer e identificar as principais moléculas expressas no intestino de uma espécie de carrapato argasídeo após o repasto sanguíneo, através de uma análise transcritômica descritiva do intestino de fêmeas ingurgitadas de O. mimon, utilizando um sequenciamento de RNA de nova geração da plataforma 454. Além de inferir a relação filogenética de carrapatos através de um conjunto de dados transcritômicos. O transcriptoma do intestino revelou diversos transcritos associados com a digestão da hemoglobina, como proteinases das classes serino, cisteína, aspártica e metalo. Registramos a presença de um transcrito de uma cisteína peptidase do tipo catepsina O em carrapatos. A inferência filogenética baseada em conjunto de dados transcritos homólogos tem uma resolução topológica similar a de outros conjuntos de dados moleculares. Foram depositados no banco de dados gênico público 2213 transcritos de O. mimon. Os achados obtidos no presente estudo podem contribuir para compreensão dos importantes processos, como digestão, nutrição e imunidade dos carrapatos da família Argasidae, além de fornecer informações sobre a filogenia da ordem Ixodida.

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Section: Discussionsupporting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Landulfo

Patané

Silva

et al. 2017

Rev. Bras. Parasitol. Vet.

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Cloning and expression pattern of a heat shock cognate protein 70 gene in ticks (Haemaphysalis flava)

2017

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Ticks and tick-borne-diseases have serious public health implications, and screening feasible protein candidates for vaccines development is identified to be an effective alternative to control of tick infestations. In current study, we focused on cloning the full-length gene encoding a heat shock cognate protein 70 (Hsc70), a molecular chaperone of critical functional roles belonging to heat shock protein 70 (HSP70) family, in salivary glands of Haemaphysalis flava, namely Hf-Hsc70, and analyzing the expression of Hf-Hsc70 in different life phases, organs and ambient temperatures. Rapid amplification of cDNA ends (RACE) was performed to amplify the 5' and 3' ends of Hf-Hsc70. The expression profiles of Hf-Hsc70 were studied by semi-quantitative real-time PCR (RT-PCR). The full-length of Hf-Hsc70 was 2363 bp, and contained an ORF of 1965 bp encoding a protein of 648 amino acids. The expression levels of Hf-Hsc70 at different life phases were in the order of female larvae < female fully engorged nymphs < male adult ticks < female full engorged adult ticks < female half engorged adult ticks. The relative expression of Hf-Hsc70 in salivary glands was steadily higher than that in midguts (p < 0.05) regardless of feeding status. A 3-h of heat stress did not significantly induce the up-regulation of Hf-Hsc70 transcription. These results indicated that Hf-Hsc70 was a constitutive form of HSP70 family, and its expression pattern in different life phases and organs suggested a possible role in blood feeding, which would further make Hsc70 a potential candidate for the development of vaccines against ticks.

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Cathepsin L—a novel cysteine protease from Haemaphysalis flava Neumann, 1897

Sun

et al. 2019

Parasitol Res

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De novo assembly and analysis of midgut transcriptome of Haemaphysalis flava and identification of genes involved in blood digestion, feeding and defending from pathogens

Cited by 34 publications

References 68 publications

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Gut transcriptome analysis on females of Ornithodoros mimon (Acari: Argasidae) and phylogenetic inference of ticks

Cloning and expression pattern of a heat shock cognate protein 70 gene in ticks (Haemaphysalis flava)

Cathepsin L—a novel cysteine protease from Haemaphysalis flava Neumann, 1897

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