De novo transcriptome assembly and comparative transcriptomic analysis provide molecular insights into low temperature stress response of Canarium album

Abstract

A de novo transcriptome analysis was performed in C. album, a temperature sensitive fruit tree in China, after treatment with varied temperatures. A total number of 168,385 transcripts were assembled, comprising of 109,439 unigenes, of which 70,530 were successfully annotated. Compared with control check group (CK), which was treated under 25 °C, the chilling stress (4 °C) treated group (CT), showed about 2810 up-regulated and 2567 down-regulated genes. Whereas, group treated under freezing (− 3 °C) stress (FT) showed an up-regulation and a down-regulation of 1748 and 1459 genes, respectively. GO classification analysis revealed that DEGs related to metabolic processes, single-organism metabolic process, and catalytic activity are significantly enriched in both CT and FT conditions. KEGG pathway enrichment analysis for both CT and FT treatments showed an enrichment of genes encoding or related to glycine/serine and threonine metabolism, alpha-linolenic acid metabolism, carotenoid biosynthesis, photosynthesis-antenna proteins, and circadian rhythm. However, genes related to photosynthesis, carbon fixation in photosynthetic organisms, glutathione metabolism, pyruvate metabolism, nicotinate and nicotinamide metabolism were specifically enriched in CT condition. Nevertheless, FT treatment induced genes related to plant-pathogen interaction, linoleic acid metabolism, plant hormone signal transduction and pentose phosphate pathway. Many of the genes involved in plant hormone signal transduction showed significantly different expression in both FT and CT conditions. However, the change was more evident in FT. Here we present the first of the reports for a de novo transcriptomic analysis in C. album, suggesting that the plant shows differential responses in chilling and freezing temperatures, where the hormone signaling and transduction contribute greatly to FT responses. Our study thus paves way for future research regarding functions of these potentially identified genes.

Discovery of gene regulation mechanisms associated with uniconazole-induced cold tolerance in banana using integrated transcriptome and metabolome analysis

“…Linoleic acid is a liquid unsaturated fatty acid that plays an indispensable role in maintaining membrane integrity under cold stress [49]. Linoleic acid metabolism is significantly enhanced in the leaves of Canarium album, rice, and Rhododendron chrysanthum in response to cold stress [32,50,51]. Linolenic acid, which is converted from linoleic acid, is a precursor of JA synthesis [52].…”

Chromosome-level genome assembly and demographic history of Euryodendron excelsum in monotypic genus endemic to China

“… 77 , 78 Transcriptome analyses in different plants also indicated that genes related to glycine metabolic process were major responsive genes in cold conditions. 79 , 80 …”

De Novo Transcriptome Analysis by PacBio SMRT-Seq and Illumina RNA-Seq Provides New Insights into Polyphenol Biosynthesis in Chinese Olive Fruit

“…To date, transcriptomic techniques have been successfully applied in C. album [64][65][66], and the chloroplast genome with a 163,140 bp of C. album has been reported [67], the comprehensive genomic and full-length transcriptomic information is still poorly understood. The importance of fruit quality and the exceptional bioactive properties in C. album have long been recognized; hence, we constructed a full-length transcriptome of C. album by using PacBio Sequel and Illumina RNA-seq here to increase our understanding of the vital and dynamic process of C. album fruit development.…”