De novo transcriptome sequencing and analysis of <i>Anisakis pegreffii</i> (Nematoda: Anisakidae) third-stage and fourth stage larvae

Nam, U-Hwa; Kim, Jong‐Oh; Kim, Jeong-Ho

doi:10.21307/jofnem-2020-041

Cited by 8 publications

(9 citation statements)

References 52 publications

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“…Despite this, in vitro culture of Anisakis species constitutes a useful tool in the study of developmental stages in the nematode life cycle. In several recent studies, in vitro culture was employed to analyse the transcriptome and gene expression in L4 larvae of A. simplex (s.s.), A. pegreffii and their hybrids, as well as for morphological and molecular characterization [ 9 , 37 , 38 ].…”

Section: Discussionmentioning

confidence: 99%

Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters

Roca-Geronès

Alcover

Godínez-González

et al. 2021

Animals

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The sibling species Anisakis simplex (s.s.) and Anisakis pegreffii are parasites of marine mammals and fish worldwide and the main causative agents of human anisakiasis. In sympatric areas, a hybrid genotype between the two species has been identified, mainly in third-stage larvae, but rarely in fourth-stage and adult forms. The aim of this study was to confirm the presence of hybrid genotypes in larvae parasitizing fish caught in sympatric and allopatric Spanish marine waters, the North-East Atlantic and West Mediterranean, respectively, and to study possible differences in the growth behaviour between genotypes. Of the 254 molecularly analysed larvae, 18 were identified as hybrids by PCR-RFLP analysis of the rDNA ITS region, 11 of which were subsequently confirmed by EF1 α-1 nDNA gene sequencing. These results therefore indicate an overestimation of hybrid genotypes when identification is based only on the ITS region. We also report the detection of a hybrid specimen in a host from the West Mediterranean, considered an allopatric zone. Additionally, fourth-stage larvae with a hybrid genotype were obtained in vitro for the first time, and no differences were observed in their growth behaviour compared to larvae with A. simplex (s.s.) and A. pegreffii genotypes.

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Section: Discussionmentioning

confidence: 99%

Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters

Roca-Geronès

Alcover

Godínez-González

et al. 2021

Animals

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“…Collagen synthesis-related genes were highly expressed in L4, suggesting the active biosynthesis of collagen during molting process. The same authors have recently published related studies based on the RNA-seq and de novo assembly of A. pegreffii L3 and L4 transcriptome profiles [ 44 ].…”

Section: Transcriptomic Analyses: Studies On Specific Developmentamentioning

confidence: 99%

Advances in Omic Studies Drive Discoveries in the Biology of Anisakid Nematodes

D’Amelio¹,

Lombardo²,

Pizzarelli³

et al. 2020

Genes

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Advancements in technologies employed in high-throughput next-generation sequencing (NGS) methods are supporting the spread of studies that, combined with advances in computational biology and bioinformatics, have greatly accelerated discoveries within basic and biomedical research for many parasitic diseases. Here, we review the most updated “omic” studies performed on anisakid nematodes, a family of marine parasites that are causative agents of the fish-borne zoonosis known as anisakiasis or anisakidosis. Few deposited data on Anisakis genomes are so far available, and this still hinders the deep and highly accurate characterization of biological aspects of interest, even as several transcriptomic and proteomic studies are becoming available. These have been aimed at discovering and characterizing molecules specific to peculiar developmental parasitic stages or tissues, as well as transcripts with pathogenic potential as toxins and allergens, with a broad relevance for a better understanding of host–pathogen relationships and for the development of reliable diagnostic tools.

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“…The A. pegreffii transcriptome here obtained was assembled with those RNA-seq data sets from the third larval stage of the parasite species. The single transcriptome available from the fourth stage larva of A. pegreffii [ 8 ] was not included in this analysis because the main aim of this analysis was to provide a robust and "ready to use'' transcriptome of the infective stage (third larval stage) of the parasite also provoking the zoonotic disease (anisakiasis) to humans.…”

Section: Limitationsmentioning

confidence: 99%

De novo transcriptome assembly and annotation of the third stage larvae of the zoonotic parasite Anisakis pegreffii

et al. 2022

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Objectives Anisakis pegreffii is a zoonotic parasite requiring marine organisms to complete its life-history. Human infection (anisakiasis) occurs when the third stage larvae (L3) are accidentally ingested with raw or undercooked infected fish or squids. A new de novo transcriptome of A. pegreffii was here generated aiming to provide a robust bulk of data to be used for a comprehensive "ready-to-use" resource for detecting functional studies on genes and gene products of A. pegreffii involved in the molecular mechanisms of parasite-host interaction. Data description A RNA-seq library of A. pegreffii L3 was here newly generated by using Illumina TruSeq platform. It was combined with other five RNA-seq datasets previously gathered from L3 of the same species stored in SRA of NCBI. The final dataset was analyzed by launching three assembler programs and two validation tools. The use of a robust pipeline produced a high-confidence protein-coding transcriptome of A. pegreffii. These data represent a more robust and complete transcriptome of this species with respect to the actually existing resources. This is of importance for understanding the involved adaptive and immunomodulatory genes implicated in the “cross talk” between the parasite and its hosts, including the accidental one (humans).

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De novo transcriptome sequencing and analysis of Anisakis pegreffii (Nematoda: Anisakidae) third-stage and fourth stage larvae

Cited by 8 publications

References 52 publications

Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters

Hybrid Genotype of Anisakis simplex (s.s.) and A. pegreffii Identified in Third- and Fourth-Stage Larvae from Sympatric and Allopatric Spanish Marine Waters

Advances in Omic Studies Drive Discoveries in the Biology of Anisakid Nematodes

De novo transcriptome assembly and annotation of the third stage larvae of the zoonotic parasite Anisakis pegreffii

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