De novo transcriptome sequencing and comparative analysis of Haemaphysalis flava Neumann, 1897 at larvae and nymph stages

Guo, Jiaying; Sun, Yali; Luo, Xiaoying; Li, Muxiao; He, Pei; He, Li; Zhao, Junlong

doi:10.1016/j.meegid.2019.104008

Cited by 9 publications

(7 citation statements)

References 52 publications

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“…For engorged female H. longicornis , ~53 million reads generated 65,916 contigs, of which 23,339 could be annotated (Niu et al, 2019 ). Whole body transcriptomes for larvae (64,474,326) and nymphs (81,612,022) were also generated (Guo et al, 2019 ). This resulted in 536,336 transcripts and 440,896 unigenes.…”

Section: Next-generation Sequencing Of Whole Body Transcriptomesmentioning

confidence: 99%

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Mans

2020

Front. Cell. Infect. Microbiol.

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Species have definitive genomes. Even so, the transcriptional and translational products of the genome are dynamic and subject to change over time. This is especially true for the proteins secreted by ticks at the tick-host feeding interface that represent a complex system known as the sialoverse. The sialoverse represent all of the proteins derived from tick salivary glands for all tick species that may be involved in tick-host interaction and the modulation of the host's defense mechanisms. The current study contemplates the advances made over time to understand and describe the complexity present in the sialoverse. Technological advances at given periods in time allowed detection of functions, genes, and proteins enabling a deeper insight into the complexity of the sialoverse and a concomitant expansion in complexity with as yet, no end in sight. The importance of systematic classification of the sialoverse is highlighted with the realization that our coverage of transcriptome and proteome space remains incomplete, but that complete descriptions may be possible in the future. Even so, analysis and integration of the sialoverse into a comprehensive understanding of tick-host interactions may require further technological advances given the high level of expected complexity that remains to be uncovered.

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Section: Next-generation Sequencing Of Whole Body Transcriptomesmentioning

confidence: 99%

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Mans

2020

Front. Cell. Infect. Microbiol.

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“…The most abundantly annotated functions were binding activity, such as protein binding activity (n=211), ATP binding activity (n=185) and metal ion binding (n=183) which coincided with transcriptomes of other ixodid tick species [42,43]. The remaining annotated functions were as follows: structural constituent of ribosome (n=210), RNA binding (n=182), oxidoreductase activity (n=170), zinc ion binding (n=125), DNA binding (n=121), hydrolase activity (n=106), nucleic acid binding (n=98).…”

Section: Gene Ontology (Go) Classi Cationmentioning

confidence: 93%

De Novo Assembly and Analysis of Transcriptome of the Dermacentor Marginatus Genes Differentially Expressed After Blood Feeding and Long-term Starvation

Yuan

et al. 2020

Preprint

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Background The Ixodid tick Dermacentor marginatus is a vector of many pathogens wide spread in Eurasia. Study of gene targets of the tick species provides insight to find novel tick protective antigen for drug development and vaccine targets. Methods To obtain a broader picture of gene sequences and changes in expression level, the RNA-seq was performed to obtain the whole-body transcriptome data of D. marginatus adult female ticks after engorgement and long-term starvation. Subsequently, the real-time quantitative PCR (RT-qPCR) was applied to validate the RNA-seq data. Results The RNA-seq produced 30251 unigenes, of which 32% were annotated using Trinity. Gene expression was compared among groups differed by status as newly molted, starved and engorged female adult ticks. Nearly 1/3 of the unigenes in each group were differentially expressed compared to the other two group, and the most numerous were genes encoding proteins involved in catalytic and binding activities and apoptosis. Selected up-regulated differentially expressed genes in each group associated to protein, lipids, carbohydrate and chitin metabolism. Blood feeding and long-term starvation also caused genes differentially expressed in the defense response and antioxidant response. RT-qPCR results indicated 6 differentially expressed transcripts showed similar trends in expression changes with RNA-seq results confirming that the gene expression profiles in transcriptome data is in consistent with RT-qPCR validation. Conclusions Obtaining the sequence information of D. marginatus and characterizing the expression pattern of the genes involved in blood feeding and during starvation would be helpful in understanding molecular physiology of D. marginatus and provides data for anti-tick vaccine and drug development for controlling the tick.

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“…The most abundantly annotated functions were binding activity, such as protein binding activity (n = 211), ATP binding activity (n = 185) and metal ion binding (n = 183) which coincided with transcriptomes of other ixodid tick species [47,48]. The remaining annotated functions were as follows: structural constituent of ribosome (n = 210), RNA binding (n = 182), oxidoreductase activity (n = 170), zinc ion binding (n = 125), DNA binding (n = 121), hydrolase activity (n = 106), nucleic acid binding (n = 98).…”

Section: Gene Ontology (Go) Classi Cationmentioning

confidence: 93%

De novo assembly and analysis of the transcriptome of the Dermacentor marginatus genes differentially expressed after blood feeding and long-term starvation

Yuan

et al. 2020

Preprint

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Background: The ixodid tick Dermacentor marginatus is a vector of many pathogens wide spread in Eurasia. Studies of gene sequence on many tick species have greatly increased the information on tick protective antigen which might have the potential to function as effective vaccine candidates or drug targets for eco-friendly acaricide development. In the current study, RNA-seq was applied to identify D. marginatus sequences and analyze differentially expressed unigenes.Methods: To obtain a broader picture of gene sequences and changes in expression level, RNA-seq was performed to obtain the whole-body transcriptome data of D. marginatus adult female ticks after engorgement and long-term starvation. Subsequently, the real-time quantitative PCR (RT-qPCR) was applied to validate the RNA-seq data.Results: RNA-seq produced 30,251 unigenes, of which 32% were annotated. Gene expression was compared among groups that differed by status as newly molted, starved and engorged female adult ticks. Nearly one third of the unigenes in each group were differentially expressed compared to the other two groups, and the most numerous were genes encoding proteins involved in catalytic and binding activities and apoptosis. Selected up-regulated differentially expressed genes in each group were associated to protein, lipids, carbohydrate and chitin metabolism. Blood-feeding and long-term starvation also caused genes differentially expressed in the defense response and antioxidant response. RT-qPCR results indicated 6 differentially expressed transcripts showed similar trends in expression changes with RNA-seq results confirming that the gene expression profiles in transcriptome data is in consistent with RT-qPCR validation.Conclusions: Obtaining the sequence information of D. marginatus and characterizing the expression pattern of the genes involved in blood feeding and during starvation would be helpful in understanding molecular physiology of D. marginatus and provides data for anti-tick vaccine and drug development for controlling the tick.

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De novo transcriptome sequencing and comparative analysis of Haemaphysalis flava Neumann, 1897 at larvae and nymph stages

Cited by 9 publications

References 52 publications

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

Quantitative Visions of Reality at the Tick-Host Interface: Biochemistry, Genomics, Proteomics, and Transcriptomics as Measures of Complete Inventories of the Tick Sialoverse

De Novo Assembly and Analysis of Transcriptome of the Dermacentor Marginatus Genes Differentially Expressed After Blood Feeding and Long-term Starvation

De novo assembly and analysis of the transcriptome of the Dermacentor marginatus genes differentially expressed after blood feeding and long-term starvation

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