De Novo Transcriptome Sequencing in <i>Trigonella foenum‐graecum</i> L. to Identify Genes Involved in the Biosynthesis of Diosgenin

Vaidya, Kanak; Ghosh, Arpita; Kumar, Vinay; Chaudhary, Spandan; Srivastava, Navin; Katudia, Kalpesh; Tiwari, Tanushree; Chikara, Surendra K.

doi:10.3835/plantgenome2012.08.0021

Cited by 47 publications

(30 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The few data available on fenugreek kariotype and genome have identified the somatic chromosome numbers of Trigonella taxa as 2n = 14, 16, 30 and 46 along with B chromosomes Vaidya et al, 2013). The C-value of fenugreek was found to be 0.7, which is 1.5 fold higher than the values of model legumes Lotus corniculatus L. var.…”

Section: Fenugreek Geneticsmentioning

confidence: 88%

“…Lotus japonicus (Regel) K. Larsen] and barrel (Medicago truncatula Gaertn. ); genome size of both these species is around 470Mbp, whereas the genome size of fenugreek is around 685Mbp (Young et al, 2003;Vaidya et al, 2013). Despite fenugreek is an important medicinal plant, its whole genome sequencing has not been carried out yet, and only transcriptome sequencing data were published earlier by our research group.…”

Section: Fenugreek Geneticsmentioning

confidence: 99%

“…Despite fenugreek is an important medicinal plant, its whole genome sequencing has not been carried out yet, and only transcriptome sequencing data were published earlier by our research group. The transcriptome contains 42 million high quality reads, with 18,333 transcripts functionally annoted and 6,775 transcripts related to metabolic pathways including diosgenin biosynthesis (Vaidya et al, 2013). In particular, targeting transcriptome sequencing of fenugreek has been fenugreek was not studied in detail in the past, even if, since the last decades, its biological activities are seeking attention of the scientific community.…”

Section: Fenugreek Geneticsmentioning

confidence: 99%

“…In India, fenugreek is cultivated mainly in Rajasthan (around 80% of the national production), Gujarat, Uttaranchal, Uttar Pradesh, Madhya Pradesh, Maharashtra, Haryana and Punjab. Petropoulos (1973) studied by Vaidya et al in order to identify the genes of diosgenin pathway (Vaidya et al, 2013). Studies on fenugreek genome should be improved, since they could provide information about the bioactive phytochemicals of this important medicinal plant.…”

Section: Fenugreek Plantmentioning

confidence: 99%

“…This plant seeds were reported to contain 35% alkaloids, 10% flavonoids (100 mg per g of fenugreek seeds), 4.8% saponins and 0.2-0.9% diosgenin (Jani, 2009;Meghwal, 2012;Vaidya, 2013). Alkaloids, along with some other volatile compounds, are mainly responsible for the bitter taste and typical aroma of fenugreek (Kumar, 2012, Faeste, 2009.…”

Section: Fenugreek Phytochemicals and Nutrientsmentioning

confidence: 99%

See 4 more Smart Citations

Review on Fenugreek (Trigonella foenum-graecum L.) and its Important Secondary Metabolite Diosgenin

Chaudhary¹,

Chaudhary²,

Chikara³

et al. 2018

Not Bot Horti Agrobo

Self Cite

View full text Add to dashboard Cite

Fenugreek (Trigonella foenum-graecum L.) is a medicinal plant used worldwide since ancient times. Its use as smelling agent and spice was documented since 15 th century. The genus Trigonella includes around 260 species diffused worldwide and belonging to Fabaceae family. In the last decades, a number of studies highlighted the biological activities and therapeutic properties of this species mainly attributed to bioactive secondary metabolites such as alkaloids, flavonoids, steroids and saponins. In particular, diosgenin, a steroidal saponin, has been investigated for its medicinal uses and fenugreek has been reported as source of raw material for the production of steroidal hormones. This review article focuses on the cultivation, genetics, ecophysiology and traditional uses of fenugreek, as well as on its medicinal properties, phytochemical and nutrient contents. Extraction procedures and pharmacological activities of diosgenin are also reviewed, as well as methods for its chemical analyses. This review focuses on the medicinal importance of Fenugreek and its important secondary metabolite diosgenin. The review article complies the results of pre-clinical studies conducted to establish the various medicinal applications of diosgenin. This will help researcher to discover fundamental role of diosgenin as a potential product for drug manufacturers and use of fenugreek as a source of diosgenin.

show abstract

Section: Fenugreek Geneticsmentioning

confidence: 88%

Section: Fenugreek Geneticsmentioning

confidence: 99%

Section: Fenugreek Geneticsmentioning

confidence: 99%

Section: Fenugreek Plantmentioning

confidence: 99%

Section: Fenugreek Phytochemicals and Nutrientsmentioning

confidence: 99%

See 3 more Smart Citations

Review on Fenugreek (Trigonella foenum-graecum L.) and its Important Secondary Metabolite Diosgenin

Chaudhary¹,

Chaudhary²,

Chikara³

et al. 2018

Not Bot Horti Agrobo

Self Cite

View full text Add to dashboard Cite

show abstract

New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

Mohammadi

Mashayekh

Rashidi-Monfared

et al. 2019

Phytochemical Analysis

View full text Add to dashboard Cite

Introduction Throughout history, thousands of medicinal and aromatic plants have been widely utilised by people worldwide. Owing to them possessing of valuable compounds with little side effects in comparison with chemical drugs, herbs have been of interest to humans for a number of purposes. Diosgenin, driven from fenugreek, Trigonella foenum‐graecum L., has extensively drawn scientist's attention owing to having curable properties and being a precursor of steroid hormones synthesis. Nonetheless, complete knowledge about the biosynthesis pathway of this metabolite is still elusive. Objective In the present research, we isolated the full‐length CDS of 14 genes involving in diosgenin formation and measured their expression rate in various genotypes, which had illustrated different amount of diosgenin. Methodology The genes were successfully isolated, and functional motifs were also assessed using in silico approaches. Results Moreover, combining transcript and metabolite analysis revealed that there are many genes playing the role in diosgenin formation, some of which are highly influential. Among them, ∆24‐reductase, which converts cycloartenol to cycloartanol, is the first‐committed and rate‐limiting enzyme in this pathway. Additionally, no transcripts indicating to the presence or expression of lanosterol synthase were detected, contradicting the previous hypothesis about the biosynthetic pathway of diosgenin in fenugreek. Conclusion Considering all these, therefore, we propose the most possible pathway of diosgenin. This knowledge will then pave the way toward cloning the genes as well as engineering the diosgenin biosynthesis pathway.

show abstract

Biotechnological Approaches for Genetic Improvement of Fenugreek (Trigonella foenum-graceum L.)

Aasım

Baloch

Bakhsh

et al. 2018

Biotechnological Approaches for Medicinal and Aromatic Plants

View full text Add to dashboard Cite

De Novo Transcriptome Sequencing in Trigonella foenum‐graecum L. to Identify Genes Involved in the Biosynthesis of Diosgenin

Abstract: Trigonella foenum-graecum

Cited by 47 publications

References 31 publications

Review on Fenugreek (Trigonella foenum-graecum L.) and its Important Secondary Metabolite Diosgenin

Review on Fenugreek (Trigonella foenum-graecum L.) and its Important Secondary Metabolite Diosgenin

New insights into diosgenin biosynthesis pathway and its regulation in Trigonella foenum‐graecum L.

Biotechnological Approaches for Genetic Improvement of Fenugreek (Trigonella foenum-graceum L.)

Contact Info

Product

Resources

About