Debridement Effect on Periodontal Pathogen <i>Porphyromonas gingivalis</i> Cultured on Titanium by Application of Atmospheric-Pressure Plasma

Abstract

The debridement treatments of dental implants are very important in longterm maintenance after implant placement in a patient. Deposition of periodontal pathogens around the implant surface has a high risk of causing periimplantitis. The aim of this study was to evaluate the extent of elimination of Porphyromonas gingivalis, known as representative periodontopathic bacteria, from titanium, which has been the main material used for dental implants. Assuming that the debridement processing of dental implants removes periodontal bacteria, one of the methods for removing bacteria deposited on titanium is considered to be plasma irradiation. Irradiation with atmosphericpressure plasma was carried out against periodontopathic bacteria cultured and deposited on the surface of a titanium disk. After the plasma irradiation, the reduction of the number of bacteria re-cultured for 24 hours was evaluated. The number of viable bacteria on the titanium surface was estimated by an ATP-bioluminescent assay. Viable cells after the plasma irradiation were reduced to 1.5% or less compared to the untreated group. As one of the methods of debridement in general dental treatments, atmospheric-pressure plasma has proved to be an effective method to remove adverse prognostic factors in dental patients.

The bactericidal effect of an atmospheric-pressure plasma jet onPorphyromonas gingivalisbiofilms on sandblasted and acid-etched titanium discs

“…To determine the proper treatment time of He-APPJ, we designed an experimental group with 3- and 5-minute treatments and observed a significant decrease in the number of bacteria as the treatment time increased. The treatment time required to completely eradicate bacteria depends on the power setting of the plasma, discharge gas, bacterial type, type of medium, and the distance between the cell and plasma tip [2428]. In addition to this study, Miura et al [28] also tested the bactericidal effect of APPJs on P. gingivalis with different plasma durations and distances on machined surfaces in an in vitro setting.…”

“…The treatment time required to completely eradicate bacteria depends on the power setting of the plasma, discharge gas, bacterial type, type of medium, and the distance between the cell and plasma tip [2428]. In addition to this study, Miura et al [28] also tested the bactericidal effect of APPJs on P. gingivalis with different plasma durations and distances on machined surfaces in an in vitro setting. However, they did not suggest a proper treatment time for P. gingivalis .…”