DEC‐205 expression on migrating dendritic cells in afferent lymph

Abstract

SUMMARYPrevious studies have identi®ed a 210 000-molecular weight molecule expressed at a high level on the surface of dendritic cells (DCs) in afferent lymph of cattle and evident on cells with the morphology of DCs in lymphoid tissues. Expression is either absent from other immune cells or is present at a lower level. The molecular weight and cellular distribution suggested that the molecule, called bovine WC6 antigen (workshop cluster), might be an orthologue of human DEC-205 (CD205). To establish whether this was the case, the open reading frame of bovine DEC-205 was ampli®ed, by polymerase chain reaction, from thymic cDNA (accession no. AY264845). The cDNA sequence of bovine DEC-205 had 86% and 78% nucleic acid identity with human and mouse molecules, respectively. COS-7 cells transfected with a plasmid containing the cattle DEC-205 coding region expressed a molecule that stained with WC6-speci®c monoclonal antibody, showing that ruminant WC6 is an orthologue of DEC-205. Twocolour¯ow cytometry of mononuclear cells from afferent lymph draining cattle skin, and from blood, con®rmed the high level of expression on large cells in lymph that were uniformly DC-LAMP positive and major histocompatibility complex class II positive. Within this DC-LAMP population were subpopulations of cells that expressed the mannose receptor or SIRPa. The observations imply that DCs in afferent lymph are all high , but not a uniform population of homogeneous mature DCs.

Immunohistochemical Detection of Dendritic Cell Markers in Cattle

“…8,18 In addition, bovine CD205 has been previously described as the WC6 antigen, showing a strong expression on ALDCs. 10 In this study, we used a monoclonal antibody (clone CNA.42) for the labeling of FDCs 34 and a polyclonal antibody for the detection of S100 protein, which is expressed in a wide variety of cell types, including FDCs. 17,23 Because of the complicated interactions between the distinct cells associated with both normal and pathologic mechanisms of action of the immune system, it is of critical interest to examine DCs not only in cell culture but also in vivo.…”

Differential Effects of Viral Vectors on Migratory Afferent Lymph Dendritic CellsIn VitroPredict Enhanced ImmunogenicityIn Vivo

“…Afferent lymphatic DC (ALDC) represent a major population of migrating DC (11,12,19,60) with functional and phenotypic heterogeneity. In cattle, various populations of skin-draining ALDC have been described by their differential expression of SIRP␣ (CD172a), CD11a, CD26, and CD13 (4,19,20,30).…”

Antigen presenting cells in mucosal sites of veterinary species

“…in comparison to draining lymph from skin [31]. In addition, the bovine homologue to human CD205 was shown to be present on both DC subsets, thereby confirming their DC nature [32].…”