Decellularization of tissues and organs

Gilbert, Thomas W.; Sellaro, Tiffany L.; Badylak, Stephen F.

doi:10.1016/j.biomaterials.2006.02.014

Cited by 1,345 publications

(1,976 citation statements)

References 83 publications

(118 reference statements)

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“…Disinfection of the tissue was carried out using 0.1% (v/v) peracetic acid for 3 h followed by extensive washing in PBS to remove residual SDS. Peracetic acid is a commonly used disinfection agent and also acts as a decellularisation agent (Gilbert et al, 2006). Peracetic acid is also widely used to disinfect surgical instruments due to excellent anti‐viral properties (Rutala and Weber, 2004).…”

Section: Resultsmentioning

confidence: 99%

“…This significant reduction was thought to be due to the use of SDS. The absence of GAGs reduces the restriction on water flow and causes a reduction in the water retention of the tissue, which also facilitates the diffusion of decellularisation solutions to remove soluble cellular components (Gilbert et al, 2006). The loss of GAGs has been associated with a reduction in the ultimate tensile strength of the tissue (Partington et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

“…These treatments are used to solubilise both cytoplasmic proteins and nuclear cellular membranes. Additional enzymatic treatments such as nucleases are added for the removal of the cellular debris (Crapo et al, 2011; Gilbert et al, 2006). During the decellularisation process proteases are released from lysed cells, which can cause damage to the native ECM ultrastructure.…”

Section: Introductionmentioning

confidence: 99%

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Decellularisation and histological characterisation of porcine peripheral nerves

Žilić

Wilshaw

Haycock

2016

Biotech & Bioengineering

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Peripheral nerve injuries affect a large proportion of the global population, often causing significant morbidity and loss of function. Current treatment strategies include the use of implantable nerve guide conduits (NGC's) to direct regenerating axons between the proximal and distal ends of the nerve gap. However, NGC's are limited in their effectiveness at promoting regeneration Current NGCs are not suitable as substrates for supporting either neuronal or Schwann cell growth, as they lack an architecture similar to that of the native extracellular matrix (ECM) of the nerve. The aim of this study was to create an acellular porcine peripheral nerve using a novel decellularisation protocol, in order to eliminate the immunogenic cellular components of the tissue, while preserving the three‐dimensional histoarchitecture and ECM components. Porcine peripheral nerve (sciatic branches were decellularised using a low concentration (0.1%; w/v) sodium dodecyl sulphate in conjunction with hypotonic buffers and protease inhibitors, and then sterilised using 0.1% (v/v) peracetic acid. Quantitative and qualitative analysis revealed a ≥95% (w/w) reduction in DNA content as well as preservation of the nerve fascicles and connective tissue. Acellular nerves were shown to have retained key ECM components such as collagen, laminin and fibronectin. Slow strain rate to failure testing demonstrated the biomechanical properties of acellular nerves to be comparable to fresh controls. In conclusion, we report the production of a biocompatible, biomechanically functional acellular scaffold, which may have use in peripheral nerve repair. Biotechnol. Bioeng. 2016;113: 2041–2053. © 2016 The Authors. Biotechnology and Bioengineering published by Wiley Periodicals, Inc.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Decellularisation and histological characterisation of porcine peripheral nerves

Žilić

Wilshaw

Haycock

2016

Biotech & Bioengineering

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“…12 However, many other decellularization methods have been reported for tissue engineering including physical, chemical, and enzymatic based approaches. 11 …”

Section: Discussionmentioning

confidence: 99%

“…10 Although there are many decellularization methods available at the time of writing including physical, chemical, enzymatic, and proteinase approaches, however high pH alone may be able to achieve sufficient decellularization. 11 …”

Section: Introductionmentioning

confidence: 99%

Sodium hydroxide based non-detergent decellularizing solution for rat lung

et al. 2018

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Lung transplantation is the last option for the treatment of end stage chronic lung disorders. Because the shortage of donor lung organs represents the main hurdle, lung regeneration has been considered to overcome this hurdle. Recellularization of decellularized organ scaffold is a promising option for organ regeneration. Although detergents are ordinarily used for decellularization, other approaches are possible. Here we used high alkaline (pH12) sodium hydroxide (NaOH)-PBS solution without detergents for lung decellularization and compared the efficacy on DNA elimination and ECM preservation with detergent based decellularization solutions CHAPS and SDS. Immunohistochemical image analysis showed that cell components were removed by NaOH solution as well as other detergents. A Collagen and GAG assay showed that the collagen reduction of the NaOH group was comparable to that of the CHAPS and SDS groups. However, DNA reduction was more significant in the NaOH group than in other groups (p < 0.0001). The recellularization of HUVEC revealed cell attachment was not inferior to that of the SDS group. Ex vivo functional analysis showed 100% oxygen ventilation increased oxygen partial pressure as artificial hemoglobin vesicle-PBS solution passed through regenerated lungs in the SDS or NaOH group. It was concluded that the NaOH-PBS based decellularization solution was comparable to ordinal decellularizaton solutions and competitive in cost effectiveness and residues in the decellularized scaffold negligible, thus providing another potential option to detergent for future clinical usage.

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Biomaterials from Decellularized Tissues

Londoño

Badylak

2016

Biomaterials From Nature for Advanced Devices and Therapies

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Decellularization of tissues and organs

Cited by 1,345 publications

References 83 publications

Decellularisation and histological characterisation of porcine peripheral nerves

Decellularisation and histological characterisation of porcine peripheral nerves

Sodium hydroxide based non-detergent decellularizing solution for rat lung

Biomaterials from Decellularized Tissues

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