Decellularized Adipose Tissue: Biochemical Composition, in vivo Analysis and Potential Clinical Applications

Mohiuddin, Omair A.; Campbell, Brett; Poche, J. Nicholas; Thomas-Porch, Caasy; Hayes, Daniel; Bunnell, Bruce A.; Gimble, Jeffrey M.

doi:10.1007/5584_2019_371

Cited by 45 publications

(38 citation statements)

References 60 publications

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“…This makes adipose tissue an excellent candidate as a biomaterial for tissue engineering applications (Flynn, 2010; Song et al, 2018). Analysis of DAT has shown that it contains collagens (I, III, IV, VI, and VI), glycosaminoglycans (GAG), laminin, elastin, fibronectin, vascular endothelial growth factor (VEGF), and fibroblast growth factor (FGF) (Mohiuddin et al, 2019). In the past decade, decellularized adipose tissue (DAT) has been proven as a versatile scaffold, which is compatible with several different cell types and useful for a wide range of potential clinical applications (Mohiuddin et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model

Mohiuddin

Campbell

Poche

et al. 2019

Front. Bioeng. Biotechnol.

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Critical-sized bone defects fail to heal and often cause non-union. Standard treatments employ autologous bone grafting, which can cause donor tissue loss/pain. Although several scaffold types can enhance bone regeneration, multiple factors limit their level of success. To address this issue, this study evaluated a novel decellularized human adipose tissue (DAT) hydrogel as an alternative. In this study, DAT hydrogel alone, or in combination with adipose-derived stromal/stem cells (ASC), osteo-induced ASCs (OIASC), and hydroxyapatite were tested for their ability to mediate repair of a critical-sized (3 mm) femoral defect created in C57BL/6 mice. Micro-computed tomography results showed that all DAT hydrogel treated groups significantly enhanced bone regeneration, with OIASC + hydroxyapatite treated group displaying the most robust bone regeneration. Histological analyses revealed that all treatments resulted in significantly higher tissue areas with the relative mineralized tissue area significantly increased at 12 weeks; however, cartilaginous content was lowest among treatment groups with OIASC. Immunohistochemical analyses showed that DAT hydrogel enhanced collagen I and osteopontin expression, while the addition of OIASCs to the hydrogel reduced collagen II levels. Thus, DAT hydrogel promotes bone regeneration in a critical-sized femoral defect model that is further enhanced in the presence of OIASCs and hydroxyapatite.

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Section: Introductionmentioning

confidence: 99%

Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model

Mohiuddin

Campbell

Poche

et al. 2019

Front. Bioeng. Biotechnol.

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“…These methods combine biological (enzyme digestion), chemical, and physical processing steps to achieve the uniform and consistent manufacture of protein scaffolds depleted of contaminating genomic DNA and lipids. Obatala scientists, in collaboration with partners at Pennsylvania State University (PSU), Tulane University, and Western Ontario University, have developed and published on decellularized human adipose tissue-derived extracellular matrix (ECM) hydrogel, commercially termed “AdipoGel™” [ 70 , 83 , 84 , 85 , 86 ]. This hydrogel or scaffold is prepared using a combination of chemical and mechanical processing followed by protease digestion as a final modification prior to sterilization.…”

Section: Extracellular Matrix (Ecm) From Decellularized Adipose Timentioning

confidence: 99%

“…This hydrogel or scaffold is prepared using a combination of chemical and mechanical processing followed by protease digestion as a final modification prior to sterilization. In vitro, AdipoGel is capable of supporting adipose-derived stromal/stem cell (ASC) proliferation and adipogenic and osteogenic differentiation; in vivo, AdipoGel promotes critical-sized endochondral bone defect repair [ 83 , 84 , 85 ]. Furthermore, the decellularized adipose-derived scaffold can be chemically modified with thiol methacrylate and cross-linked to create a cytocompatible matrix that can be “tuned” to achieve biomechanical properties appropriate for the promotion of either soft or hard tissue regeneration [ 87 ].…”

Section: Extracellular Matrix (Ecm) From Decellularized Adipose Timentioning

confidence: 99%

Clinical Translational Potential in Skin Wound Regeneration for Adipose-Derived, Blood-Derived, and Cellulose Materials: Cells, Exosomes, and Hydrogels

Frazier¹,

Alarcon²,

et al. 2020

Biomolecules

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Acute and chronic skin wounds due to burns, pressure injuries, and trauma represent a substantial challenge to healthcare delivery with particular impacts on geriatric, paraplegic, and quadriplegic demographics worldwide. Nevertheless, the current standard of care relies extensively on preventive measures to mitigate pressure injury, surgical debridement, skin flap procedures, and negative pressure wound vacuum measures. This article highlights the potential of adipose-, blood-, and cellulose-derived products (cells, decellularized matrices and scaffolds, and exosome and secretome factors) as a means to address this unmet medical need. The current status of this research area is evaluated and discussed in the context of promising avenues for future discovery.

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“…In addition, there are different names about DAM, including decellularized adipose tissue[ 26 , 74 - 77 ], adipose-derived matrix[ 23 , 24 ], and acellular adipose matrix[ 78 ]. For the convenience of explanation, this article collectively names DAM from adipose tissue of different sources (including human, pig, mouse, etc .).…”

Section: Overview Of Ecm/dammentioning

confidence: 99%

Decellularized adipose matrix provides an inductive microenvironment for stem cells in tissue regeneration

Yang

Qiu

Xiong

et al. 2020

WJSC

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Stem cells play a key role in tissue regeneration due to their self-renewal and multidirectional differentiation, which are continuously regulated by signals from the extracellular matrix (ECM) microenvironment. Therefore, the unique biological and physical characteristics of the ECM are important determinants of stem cell behavior. Although the acellular ECM of specific tissues and organs (such as the skin, heart, cartilage, and lung) can mimic the natural microenvironment required for stem cell differentiation, the lack of donor sources restricts their development. With the rapid development of adipose tissue engineering, decellularized adipose matrix (DAM) has attracted much attention due to its wide range of sources and good regeneration capacity. Protocols for DAM preparation involve various physical, chemical, and biological methods. Different combinations of these methods may have different impacts on the structure and composition of DAM, which in turn interfere with the growth and differentiation of stem cells. This is a narrative review about DAM. We summarize the methods for decellularizing and sterilizing adipose tissue, and the impact of these methods on the biological and physical properties of DAM. In addition, we also analyze the application of different forms of DAM with or without stem cells in tissue regeneration (such as adipose tissue), repair (such as wounds, cartilage, bone, and nerves), in vitro bionic systems, clinical trials, and other disease research.

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Decellularized Adipose Tissue: Biochemical Composition, in vivo Analysis and Potential Clinical Applications

Cited by 45 publications

References 60 publications

Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model

Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model

Clinical Translational Potential in Skin Wound Regeneration for Adipose-Derived, Blood-Derived, and Cellulose Materials: Cells, Exosomes, and Hydrogels

Decellularized adipose matrix provides an inductive microenvironment for stem cells in tissue regeneration

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