Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice

Abstract

Pericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of cardiac septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be used as an alternative to synthetic materials or xenograft in congenital septal defects repair. The availability of an allogenic decellularized pericardium could reduce complication during and after surgery and could be a valid alternative. Decellularization of allogenic tissues aims at reducing the immunogenic reaction that might trigger inflammation and tissue calcification over time. The ideal graft for congenital heart disease repair should be biocompatible, mechanically resistant, non-immunogenic, and should have the ability to growth with the patients. The aim of the present study is the evaluation of the efficacy of a new decellularization protocol of homologous pericardium, even after cryopreservation. The technique has proven to be suitable as a tissue bank procedure and highly successful in the removal of cells and nucleic acids content, but also in the preservation of collagen and biomechanical properties of the human pericardium.

A Methodological Approach for Interpreting and Comparing the Viscoelastic Behaviors of Soft Biological Tissues and Hydrogels at the Cell-Length Scale

“…In the last two decades, researchers have focused on how the mechanical properties of biological tissues and their extracellular matrixes (ECMs) influence a cell's response, investigating mostly their elastic properties [3][4][5][6][7][8][9][10]. However, soft biological tissues exhibit a time-dependent mechanical behavior characterized by significant stress relaxation in response to a prescribed deformation [11], and the viscous properties of these tissues have emerged recently as key elements that influence cellular behavior [12][13][14][15].…”