Decellularized extracellular matrix in the treatment of spinal cord injury

Jiang, Wenwei; Zhang, Xuanxuan; Yu, Shumin; Yan, Fangsu; Chen, Jiaxi; Liu, Jinyi; Dong, Chuanming

doi:10.1016/j.expneurol.2023.114506

Cited by 8 publications

(2 citation statements)

References 74 publications

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“…Many approaches to enhance CNS regeneration have used decellularized tissue from either the CNS or PNS. Spinal cord dECM is increasingly being used in traumatic spinal cord injury (SCI) and offers several advantages that are only beginning to be explored in the treatment of traumatic brain injury (TBI; Jiang et al, 2023). Wu and colleagues have shown that brain-derived dECM hydrogels provide sustained protection of structural and functional brain integrity after TBI.…”

Section: Injectable and Transplantable Decm Scaffoldsmentioning

confidence: 99%

Exploring the properties and potential of the neural extracellular matrix for next‐generation regenerative therapies

Ortega,

Soares de Aguiar,

Chandravanshi

et al. 2024

WIREs Nanomed Nanobiotechnol

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The extracellular matrix (ECM) is a dynamic and complex network of proteins and molecules that surrounds cells and tissues in the nervous system and orchestrates a myriad of biological functions. This review carefully examines the diverse interactions between cells and the ECM, as well as the transformative chemical and physical changes that the ECM undergoes during neural development, aging, and disease. These transformations play a pivotal role in shaping tissue morphogenesis and neural activity, thereby influencing the functionality of the central nervous system (CNS). In our comprehensive review, we describe the diverse behaviors of the CNS ECM in different physiological and pathological scenarios and explore the unique properties that make ECM‐based strategies attractive for CNS repair and regeneration. Addressing the challenges of scalability, variability, and integration with host tissues, we review how advanced natural, synthetic, and combinatorial matrix approaches enhance biocompatibility, mechanical properties, and functional recovery. Overall, this review highlights the potential of decellularized ECM as a powerful tool for CNS modeling and regenerative purposes and sets the stage for future research in this exciting field.This article is categorized under: Implantable Materials and Surgical Technologies > Nanotechnology in Tissue Repair and Replacement Therapeutic Approaches and Drug Discovery > Nanomedicine for Neurological Disease Implantable Materials and Surgical Technologies > Nanomaterials and Implants

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Section: Injectable and Transplantable Decm Scaffoldsmentioning

confidence: 99%

Exploring the properties and potential of the neural extracellular matrix for next‐generation regenerative therapies

Ortega,

Soares de Aguiar,

Chandravanshi

et al. 2024

WIREs Nanomed Nanobiotechnol

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“…As a sort of natural biomaterial, decellularized extracellular matrix (dECM) retains the biophysical structures, components of extracellular matrix (ECM) from the native tissues and tissue-specific bioactive factors [ 7 , 8 ]. Furthermore, the injectable dECM hydrogels can be easily prepared by following the consecutive processes of enzymatic digestion, pH neutralization and ionic balance [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Sustained delivery of NT-3 and curcumin augments microenvironment modulation effects of decellularized spinal cord matrix hydrogel for spinal cord injury repair

Chen,

Cheng,

et al. 2024

Regenerative Biomaterials

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Decellularized extracellular matrix hydrogel, especially that derived from spinal cord (DSCM hydrogel), has been actively considered as a functional biomaterial for remodeling the extracellular matrix of the native tissue, due to its unique characteristics in constructing pro-regenerative microenvironment for neural stem cells (NSCs). Furthermore, DSCM hydrogel can provide multiple binding domains to growth factors and drugs. Therefore, both exogenous neurotrophic factors and anti-inflammatory drugs are highly desired to be incorporated into DSCM hydrogel, which may synergistically modulate the complex microenvironment at the lesion site after spinal cord injury (SCI). Herein, neurotrophin-3 (NT-3) and curcumin (Cur) were integrated into DSCM hydrogel for SCI therapy. Due to different affinities to the DSCM hydrogel, NT-3 underwent a controlled release manner, while curcumin released explosively within the first 24 hours, followed by rather sustained but slower release. The integration of both NT-3 and curcumin significantly enhanced NSCs proliferation and their neuronal differentiation. Meanwhile, the release of curcumin promoted macrophages polarization into anti-inflammatory subtypes, which further facilitated NSCs differentiation into neurons. The in-situ injected DSCM+NT3+Cur hydrogel exerted superior capability in alleviating the inflammatory responses in rat contused spinal cord. Compared to DSCM hydrogel alone, DSCM+NT3+Cur hydrogel more significantly promoted the recruitment of NSCs and their neuronal differentiation at the lesion site. These outcomes favored functional recovery, as evidenced by the improved hind limb movement. Overall, the bioactive DSCM hydrogel can serve as a multifunctional carrier for cooperatively release of growth factors and drugs, which significantly benefits microenvironment regulation and nerve regeneration after SCI.

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Application of Adipose Extracellular Matrix and Reduced Graphene Oxide Nanocomposites for Spinal Cord Injury Repair

Verstappen,

Bieler,

Barroca

et al. 2024

Adv Healthcare Materials

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Graphene‐based materials (GBMs) hold strong promise to restore the spinal cord microenvironment and promote functional recovery after spinal cord injury (SCI). Nanocomposites consisting of reduced graphene oxide (rGO) and adipose tissue‐derived extracellular matrix (adECM) are known to promote neuronal growth in vitro and to evoke a biocompatible response in vivo when implanted on top of the intact spinal cord. In this study, pristine adECM and adECM‐rGO nanocomposites are implanted directly after hemisection SCI in rats. Scaffolds composed of collagen type I (COL) are applied as negative control, based on evidence that COL triggers integrin‐mediated astrogliosis. However, COL scaffolds induce orthotopic bone formation in the lesion site and are therefore excluded from further analyses. Compared to pristine adECM, adECM‐rGO nanocomposites completely restore spinal cord integrity. Macrophage‐mediated uptake and clearance of rGO remnants is observed as early as 3 weeks post‐implantation. Nanocomposites show an elevated presence of βIII‐tubulin‐positive axons in the host‐material interface after 8 weeks, yet scaffold penetration by axons is only occasionally observed. This is partially due to an increased expression of chondroitin sulfate proteoglycans (CSPGs) within the nanocomposites, even though reactive astrogliosis is unaltered. Despite the complete restoration of tissue architecture, adECM‐rGO treatment does not significantly improve functional recovery.

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Decellularized extracellular matrix in the treatment of spinal cord injury

Cited by 8 publications

References 74 publications

Exploring the properties and potential of the neural extracellular matrix for next‐generation regenerative therapies

Exploring the properties and potential of the neural extracellular matrix for next‐generation regenerative therapies

Sustained delivery of NT-3 and curcumin augments microenvironment modulation effects of decellularized spinal cord matrix hydrogel for spinal cord injury repair

Application of Adipose Extracellular Matrix and Reduced Graphene Oxide Nanocomposites for Spinal Cord Injury Repair

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