Deciphering bacterial social traits via diffusible signal factor (DSF) -mediated public goods in an anammox community

Guo, Yongzhao; Zhao, Yunpeng; Tang, Xi; Na, Tianxing; Pan, Juejun; Zhao, Huazhang; Liu, Sitong

doi:10.1016/j.watres.2020.116802

Cited by 47 publications

(11 citation statements)

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“…Studies have shown that the key to regulating gene transcription is that the transcription regulator could bind to gene promoters, and different transcription regulators have different binding sequences . Therefore, we could screen the regulated genes by finding the specific binding sequence of Clp, which has been used in previous studies. , Through this method, 299 genes, including a few encoding transcription factors, were found to be regulated by DSF-mediated communication for the bacterial pathogen X. campestris …”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, prediction of clp-regulated genes was conducted referring to Guo et al . Specifically, a PYTHON script was used to parse and match the promoter sequences of clp-regulated genes using the putative clp-binding motif “TGTGA-N6-TCACA” .…”

Section: Methodsmentioning

confidence: 99%

“…43−45 Moreover, a structure-based sequence alignment with Salmonella typhimurium LsrB was generated on the fold recognition server PHYRE, 46 and conserved residues important in the binding with R-THMF were examined. 41 Furthermore, prediction of clp-regulated genes was conducted referring to Guo et al 28 Specifically, a PYTHON script was used to parse and match the promoter sequences of clpregulated genes using the putative clp-binding motif "TGTGA-N6-TCACA". 47 The promoter sequence, which was arbitrarily defined as a 500 bp region upstream of an open reading frame (ORF), was obtained from a previous study.…”

Section: Bcg Retrieval Within Magsmentioning

confidence: 99%

“…For example, bacterial communication could regulate the biosynthesis of bacterial antibiotic and toxic factors, thereby inhibiting the surrounding bacteria, which could change the composition of the community . On the contrary, production or transport of “public goods”, which could be used by any member of the community, is under regulation of bacterial communication. − In other words, bacterial interactions including both cooperation and competition could be affected by communication. Studies have shown that bacterial cooperation helps bacteria resist adverse environments .…”

Section: Introductionmentioning

confidence: 99%

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Bacterial Communication Coordinated Behaviors of Whole Communities to Cope with Environmental Changes

Pan

Zhou

Tang

et al. 2023

Environ. Sci. Technol.

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Bacterial communication plays an important role in coordinating microbial behaviors in a community. However, how bacterial communication organizes the entire community for anaerobes to cope with varied anaerobic–aerobic conditions remains unclear. We constructed a local bacterial communication gene (BCG) database comprising 19 BCG subtypes and 20279 protein sequences. BCGs in anammox-partial nitrification consortia coping with intermittent aerobic and anaerobic conditions as well as gene expressions of 19 species were inspected. We found that when suffering oxygen changes, intra- and interspecific communication by a diffusible signal factor (DSF) and bis-(3′-5′)-cyclic dimeric guanosine monophosphate (c-di-GMP) changed first, which in turn induced changes of autoinducer-2 (AI-2)-based interspecific and acyl homoserine lactone (AHLs)-based intraspecific communication. DSF and c-di-GMP-based communication regulated 455 genes, which covered 13.64% of the genomes and were mainly involved in antioxidation and metabolite residue degradation. For anammox bacteria, oxygen influenced DSF and c-di-GMP-based communication through RpfR to upregulate antioxidant proteins, oxidative damage-repairing proteins, peptidases, and carbohydrate-active enzymes, which benefited their adaptation to oxygen changes. Meanwhile, other bacteria also enhanced DSF and c-di-GMP-based communication by synthesizing DSF, which helped anammox bacteria survive at aerobic conditions. This study evidences the role of bacterial communication as an “organizer” within consortia to cope with environmental changes and sheds light on understanding bacterial behaviors from the perspective of sociomicrobiology.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Bcg Retrieval Within Magsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Bacterial Communication Coordinated Behaviors of Whole Communities to Cope with Environmental Changes

Pan

Zhou

Tang

et al. 2023

Environ. Sci. Technol.

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“…QS can regulate the secretion of extracellular digestive enzymes ( Allen et al, 2016 ) to coordinate the cooperation of Pseudomonas aeruginosa . Guo et al (2021) also found that some species can provide expensive metabolites to DSF-secreting species after the addition of exogenous diffusible signal factor (DSF) to the anammox microbial co-culture system.…”

Section: Construction Of Synthetic Microbial Consortia Based On Cross...mentioning

confidence: 99%

Construction of Environmental Synthetic Microbial Consortia: Based on Engineering and Ecological Principles

Liang

Zhuang

2022

Front. Microbiol.

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In synthetic biology, engineering principles are applied to system design. The development of synthetic microbial consortia represents the intersection of synthetic biology and microbiology. Synthetic community systems are constructed by co-cultivating two or more microorganisms under certain environmental conditions, with broad applications in many fields including ecological restoration and ecological theory. Synthetic microbial consortia tend to have high biological processing efficiencies, because the division of labor reduces the metabolic burden of individual members. In this review, we focus on the environmental applications of synthetic microbial consortia. Although there are many strategies for the construction of synthetic microbial consortia, we mainly introduce the most widely used construction principles based on cross-feeding. Additionally, we propose methods for constructing synthetic microbial consortia based on traits and spatial structure from the perspective of ecology to provide a basis for future work.

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Recent Nanotechnologies to Overcome the Bacterial Biofilm Matrix Barriers

Wang

Mei

et al. 2022

Small

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Bacterial biofilms cause persistent infectious diseases and create tremendous obstacles to anti-infection treatment. [5,6] Epidemiological evidence has shown that biofilm formation poses a risk of chronic infection for clinical treatments, particularly in implant patients [7,8] such as cardiovascular grafts, fracture treatment, and orthodontic treatment. [9,10] If the biofilm infection is not cured in time, the long-term use of antibiotics to treat chronic infection may further aggravate the negative impact of antibiotics on the human body.The particular structure and components of the biofilm hinder the penetration efficiency of drugs and reduce the therapeutic effect of antibiotics, causing the hazard of bacterial biofilms. [11] In addition, some drug-resistant bacteria that inhabit the biofilms could secrete hydrolases (e.g., β-lactamases) to degrade antibiotics, making antibiotics ineffective. [12] In biofilms, the matrix, which accounts for most of the dry mass, possesses diverse functions such as the external digestive system, recycling center, gene pool, and nutrient source. [13,14] Biofilm organisms produce extracellular polymeric substances (EPS), primarily comprising exopolysaccharides, proteins, lipids, and extracellular DNA (eDNA), varying according to the environmental conditions. [15,16] EPS protects the organism from drying, oxidization, charged biocides, antibiotics, metal cations, and the host's immune defenses. [13,17,18] Additionally, EPS limits the diffusion of nutrients, creating a nutrient-deficient environment, further resulting in the slow metabolism of bacteria within the biofilm, making the bacteria less sensitive to antibiotics. [19] First, obstruction of the biofilm matrix results in extremely poor antibiotic and nutrient permeability. The aggregation of cations, such as Ca 2+ and Mg 2+ , in biofilms, promotes crosslinking between polymeric polysaccharide molecules, increasing both the viscosity and binding forces of the biofilm matrix. [20] The hydrophilic-hydrophobic properties of EPS also influence the drug penetration effect, which is related to the hydration state of EPS. [21,22] Furthermore, EPS helps immobilize bacterioplankton. The embedded bacteria in the biofilm show intense interconnections and produce stronger intercellular signal communication, a process called quorum sensing (QS). [23] Benefitting from QS, a relatively Bacterial biofilm-related infectious diseases severely influence human health. Under typical situations, pathogens can colonize inert or biological surfaces and form biofilms. Biofilms are functional aggregates that coat bacteria with extracellular polymeric substances (EPS). The main reason for the failure of biofilm infection treatment is the low permeability and enrichment of therapeutic agents within the biofilm, which results from the particular features of biofilm matrix barriers such as negatively charged biofilm components and highly viscous compact EPS structures. Hence, developing novel therapeutic strategies with enhanced biofilm penetrability is...

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Deciphering bacterial social traits via diffusible signal factor (DSF) -mediated public goods in an anammox community

Cited by 47 publications

References 54 publications

Bacterial Communication Coordinated Behaviors of Whole Communities to Cope with Environmental Changes

Bacterial Communication Coordinated Behaviors of Whole Communities to Cope with Environmental Changes

Construction of Environmental Synthetic Microbial Consortia: Based on Engineering and Ecological Principles

Recent Nanotechnologies to Overcome the Bacterial Biofilm Matrix Barriers

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