Deciphering genetic variations of proteome responses to water deficit in maize leaves

Riccardi, Frédérique; Gazeau, Pascale; Jacquemot, Marie-Pierre; Vincent, Delphine; Zivy, Michel

doi:10.1016/j.plaphy.2004.09.009

Cited by 84 publications

(47 citation statements)

References 44 publications

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“…Additionally, our transcript and protein expression data confirmed and extended findings of up-regulation by water deficit of ZmASR1 in maize leaves (Riccardi et al, 1998(Riccardi et al, , 2004. Indeed, we showed up-regulation of ZmASR1, ZmASR2, ZmASR4, and ZmASR7-1 by water deficit in leaves, although direct treatment with ABA in leaves induced elevations of ZmASR1, ZmASR3, and ZmASR4.…”

Section: Discussion Expression Of Asr Genes In Response To Water Defisupporting

confidence: 87%

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

et al. 2011

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Abscisic acid-, stress-, and ripening-induced (ASR) proteins were first described about 15 years ago as accumulating to high levels during plant developmental processes and in response to diverse stresses. Currently, the effects of ASRs on water deficit tolerance and the ways in which their physiological and biochemical functions lead to this stress tolerance remain poorly understood. Here, we characterized the ASR gene family from maize (Zea mays), which contains nine paralogous genes, and showed that maize ASR1 (ZmASR1) was encoded by one of the most highly expressed paralogs. Ectopic expression of ZmASR1 had a large overall impact on maize yield that was maintained under water-limited stress conditions in the field. Comparative transcriptomic and proteomic analyses of wild-type and ZmASR1-overexpressing leaves led to the identification of three transcripts and 16 proteins up-or down-regulated by ZmASR1. The majority of them were involved in primary and/or cellular metabolic processes, including branched-chain amino acid (BCAA) biosynthesis. Metabolomic and transcript analyses further indicated that ZmASR1-overexpressing plants showed a decrease in BCAA compounds and changes in BCAA-related gene expression in comparison with wild-type plants. Interestingly, within-group correlation matrix analysis revealed a close link between 13 decreased metabolites in ZmASR1-overexpressing leaves, including two BCAAs. Among these 13 metabolites, six were previously shown to be negatively correlated to biomass, suggesting that ZmASR1-dependent regulation of these 13 metabolites might contribute to regulate leaf growth, resulting in improvement in kernel yield.

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Section: Discussion Expression Of Asr Genes In Response To Water Defisupporting

confidence: 87%

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

et al. 2011

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“…For such a purpose, a detailed knowledge at the molecular level of the mechanisms of drought stress early perception, signal transduction and responses is necessary. It is already known that drought responses are multiple and interconnected [7]. Besides, studies in sunflower have revealed that drought tolerance is a complex trait, controlled by factors that affect leaves and roots, and alter physiological aspects of the plant [8].…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of Responses to Drought Stress in Sunflower (Helianthus annuus) Leaves by 2DE Gel Electrophoresis and Mass Spectrometry

Castillejo¹,

Maldonado²,

Ogueta³

et al. 2008

TOPROTJ

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By using a differential proteomic approach, responses to drought stress in sunflower have been studied. Two sunflower genotypes, showing different levels of tolerance to drought have been utilized. Following TCA-acetone protein extraction, the 2-DE leaf protein profile of well watered and drought stressed plants have been compared. Coomassie staining of the gels allowed visualization of around 350 well resolved spots within the 5-8 pH and 10-100 kDa ranges. Image analysis revealed the presence of both, qualitative and quantitative changes between genotypes and treatments. Differential spots were subjected to trypsin digestion and peptides were analyzed by MALDI-TOF mass spectrometry. After database search using peptide mass fingerprinting, 2 genotype-dependent and 23 (susceptible genotype) and 5 (tolerant genotype) stress-responsive protein spots were identified. The two proteins spots differentiating sunflower genotypes corresponded to phosphoglycerate kinase and glyceraldehyde-3-phosphate dehydrogenase. In response to drought conditions a general decrease in protein spots corresponding to enzymes of the photosynthesis and carbohydrate metabolism was observed in the more susceptible genotype, suggesting inhibition of the energetic metabolism. Such changes have not been observed in the tolerant genotype, indicating a normal metabolism under drought stress.

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“…In a report of Riccardi et al (1998), it also identified a protein of the same type in maize treated in water shortages and associated with the dehydration tolerance mechanisms (Riccardi et al 2004). This gene acts to ameliorate the effect of osmotic stress, such as detoxification of ROS (Veeranagamallaiah et al 2008).…”

Section: Analysis Of Salt Responsive Gene Expression (Osr40c1)mentioning

confidence: 99%

Short Communication: Altitudinal distribution of Papilionoidea (Lepidoptera) in Mount Aragats, Armenia

Zarikian

2017

Biodiversitas

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Abstract. Saputro TB, Sholihah NF, Ermavitalini D. 2017. Dynamics expression of Osr40c1 gene and growth of maize (Zea mays) calluses in responding to salt stress. . Maize (Zea mays L) is one of the most planted cereal plants worldwide. The demand of maize plant is increased over the years since its various usage in industry. Unfortunately, the production rate does not meet the demands caused by the significant reduction of plantation area for housing and the others building. The marginal saline soil area can be a promising area to grow the maize although contains a high amount of salt. The high concentration of salt can significantly reduce the plant growth and productivity. The aims of this study were to determine the tolerant level of Manding and Bluto varieties against salt stress. There were two main stages on this research. First, callus induction through in vitro culture (MS0 + 2,4-D 3 mg/L), then the calluses were subcultured on the selection medium (MS0 + 2,4-D 3 mg/L + NaCl concentrations (0, 2500, 5000 and 7500 mg/L)). Second, Q-PCR analysis was used to observe the differences of gene expression level. The result shows that NaCl stress can alter the color of callus, from yellow to brown. The percentage of surviving callus was 100% in both varieties, although the callus weight decreased along with the increment of NaCl concentrations. In 7500 mg/L, Manding has 0.023 g in fresh weight, while Bluto 0,027 gr. Interestingly, the expression of Osr40c1 gene showed different level between those two varieties. Manding had only 0.82 fold, while Bluto had 4.86 folds compared with the control. Taken together, Bluto has a better performance in high content of NaCl.

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Deciphering genetic variations of proteome responses to water deficit in maize leaves

Cited by 84 publications

References 44 publications

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

The ZmASR1 Protein Influences Branched-Chain Amino Acid Biosynthesis and Maintains Kernel Yield in Maize under Water-Limited Conditions

Proteomic Analysis of Responses to Drought Stress in Sunflower (Helianthus annuus) Leaves by 2DE Gel Electrophoresis and Mass Spectrometry

Short Communication: Altitudinal distribution of Papilionoidea (Lepidoptera) in Mount Aragats, Armenia

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