Deciphering the effect of vitamins and mineral nutrients on kiwiberry micropropagation using computer-based tools

Artificial Neural Networks Elucidated the Essential Role of Mineral Nutrients versus Vitamins and Plant Growth Regulators in Achieving Healthy Micropropagated Plants

“…The implications of this adjustment can be observed in the effect of K + over SQ. Although in our previous study, the positive effect of K + on interaction with SO 4 2− was pointed out for SQ [ 4 , 22 ], in the present study sulfate ion did not appear as a key factor affecting this parameter, probably underlining the predominant role of K + , as this ion persisted as critical for this parameter in both studies. In the present study, a strong interaction of K + with both NO 3 − and SO 4 2− was described, being necessary to have Low K + levels and High NO 3 − and SO 4 2− or vice versa , to achieve the highest results for SN, SL, and LA.…”

“…Neurofuzzy logic established the importance of this ion in the culture medium, being necessary to supply Mg 2+ at 2.44–4.50 mM ( Table 4 ) to achieve longer shoots. That optimal range is slightly higher than the one obtained for the same species in our previous studies [ 4 , 22 ]. This correction of the optimal range could be because the model now considers all the components of the medium (minerals, vitamins, and PGRs).…”

“…To that end, a strategy based on data mining was used. Data from two previous studies focused on the effect of mineral nutrients [ 4 ] and vitamins [ 22 ] on the micropropagation of Actinidia arguta were merged with the results of a new experiment focused on the effect of two PGRs. It should be noted that some modifications have been made compared to previous databases: (i) EDTA has been removed as a factor and only Fe 2+ ion is considered, (ii) shoot number (SN) and shoot length (SL) parameters have been curated to better represent the most viable shoots for subsequent stages of micropropagation (see Material and Methods).…”

“…The type or amount required for the plant remains unclear [ 21 ]. In a recent study, Arteta and coworkers [ 22 ], taking advantage of ANN tools, shed light on the role of certain vitamins such as pyridoxine, vitamin E, and Myo-inositol on the shoot number and shoot length of A. arguta .…”

“…For this, a strategy based on data mining was applied. Data from two previous studies focused on the effect of mineral nutrients [ 4 ] and vitamins [ 22 ] on the micropropagation of A. arguta were merged with the results of a new experiment focused on the effect of two PGRs. All treatments were established based on the original MS formulation.…”