Deciphering the structure of isomeric oligosaccharides in a complex mixture by tandem mass spectrometry: Photon activation with vacuum ultra-violet brings unique information and enables definitive structure assignment

Ropartz, David; Lemoine, Jérôme; Giuliani, Alexandre; Bittebière, Yann; Enjalbert, Quentin; Antoine, Rodolphe; Dugourd, Philippe; Ralet, Marie-Christine; Rogniaux, Hélène

doi:10.1016/j.aca.2013.11.018

Cited by 34 publications

(63 citation statements)

References 58 publications

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“…LE-CID only produced five cross-ring cleavages, three of which are 0,2 X fragment ions. Similar fragmentation pattern has been observed in CID results reported by Brodbelt et al [52], Rogniaux et al [179], etc. The lack of cross-ring fragmentation not only precludes the identification of sites of post-translational modifications (PTMs), but also presents the potential limitation in elucidating the branching pattern of highly branched oligosaccharide samples in the future.…”

Section: Resultssupporting

confidence: 89%

Charge Transfer Dissociation Mass Spectrometry of Biomolecules

Li¹

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Balapuwaduge Mendis, as well as "short-term" members in our laboratory-Heather Birks, Clayton Johnson, Tyler Williams, Iris Kreft and Dr. David Ropartz. All the group members have made contributions in creating a positive and collaborative laboratory atmosphere, in which the lab members help each other in both research work and daily life. All these leave me with fond and valuable memories for my graduate experience. I also would like to thank the members of my dissertation committee-Dr.

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Section: Resultssupporting

confidence: 89%

Charge Transfer Dissociation Mass Spectrometry of Biomolecules

Li¹

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“…Fragmentation of a complex mixture of thirty‐five oligogalacturonans with various degrees of polymerization and methylation derived from enzymatic digestion of a large, highly methylated, homogalacturonan from citrus fruit has been investigated by CID and UV photon activation (Ropartz et al, ). The oligogalacturonans were first separated by reversed‐phase chromatography with addition of an ion pairing reagent and examined by CID.…”

Section: Fragmentationmentioning

confidence: 99%

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Harvey

2018

Mass Spectrometry Reviews

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This review is the eighth update of the original article published in 1999 on the application of Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry to the analysis of carbohydrates and glycoconjugates and brings coverage of the literature to the end of 2014. Topics covered in the first part of the review include general aspects such as theory of the MALDI process, matrices, derivatization, MALDI imaging, fragmentation, and arrays. The second part of the review is devoted to applications to various structural types such as oligo- and poly- saccharides, glycoproteins, glycolipids, glycosides, and biopharmaceuticals. Much of this material is presented in tabular form. The third part of the review covers medical and industrial applications of the technique, studies of enzyme reactions, and applications to chemical synthesis. © 2018 Wiley Periodicals, Inc. Mass Spec Rev 37:353-491, 2018.

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“…Recently, Broadbelt and coworkers utilized UVPD to analyze O‐ linked acidic glycopeptide species from A baumannii OmpA/MotB site‐specifically, sequencing glycan, and peptide simultaneously . Additionally, Rogniaux and coworkers used tunable synchrotron radiation as the laser source (100 nm < λ < 200 nm) of UVPD to characterize the structures of isomeric oligosaccharides released from homogalacturonans originating from citrus fruit . Moreover, they implemented synchrotron radiation laser producing extreme‐energy photons (10 nm < λ < 100 nm) to MS, to characterize the complex mixtures of oligosaccharides derived from red algae Porphyra umbilicalis .…”

Section: Advanced Dissociation Techniques For Glycomicsmentioning

confidence: 99%

Advances in mass spectrometry‐based glycomics

et al. 2018

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The diversification of the chemical properties and biological functions of proteins is attained through posttranslational modifications, such as glycosylation. Glycans, which are covalently attached to proteins, play a vital role in cell activities. The microheterogeneity and complexity of glycan structures associated with proteins make comprehensive glycomic analysis challenging. However, recent advancements in mass spectrometry (MS), separation techniques, and sample preparation methods have primarily facilitated structural elucidation and quantitation of glycans. This review focuses on describing recent advances in MS-based techniques used for glycomic analysis (2012-2018), including ionization, tandem MS, and separation techniques coupled with MS. Progress in glycomics workflow involving glycan release, purification, derivatization, and separation will also be highlighted here. Additionally, the recent development of quantitative glycomics through comparative and multiplex approaches will also be described.

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Deciphering the structure of isomeric oligosaccharides in a complex mixture by tandem mass spectrometry: Photon activation with vacuum ultra-violet brings unique information and enables definitive structure assignment

Cited by 34 publications

References 58 publications

Charge Transfer Dissociation Mass Spectrometry of Biomolecules

Charge Transfer Dissociation Mass Spectrometry of Biomolecules

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2013–2014

Advances in mass spectrometry‐based glycomics

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