Decoding epigenetic cell signaling in neuronal differentiation

Vieira, Mariana Sousa; Goulart, Vânia Aparecida Mendes; Parreira, Ricardo Cambraia; Oliveira‐Lima, Onésia Cristina; Glaser, Talita; Naaldijk, Yahaira; Ferrer, Alejandra I.; Savanur, Vibha Harindra; Reyes, Paola Alicea; Sandiford, Oleta A.; Rameshwar, Pranela; Ulrich, Henning; Pinto, Mauro Cunha Xavier; Resende, Rodrigo R.

doi:10.1016/j.semcdb.2018.12.006

Cited by 13 publications

(8 citation statements)

References 209 publications

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“…The cell-type-specific 3D epigenomes revealed new laws of cis-acting element regulation of gene expression during cell fate determination, was expected to provide new reference data for brain development and neuropsychiatric disorders (Song et al, 2020). The epigenetics of iPSC-derived neurons has been extensively studied, which was limited by the lack of complex networks (Vieira et al, 2019).…”

Section: Epigenetic Modification Of Human Brain and Brain Organoidsmentioning

confidence: 99%

Genetic and Epigenetic Regulation of Brain Organoids

Wang

2022

Front. Cell Dev. Biol.

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Revealing the mechanisms of neural development and the pathogenesis of neural diseases are one of the most challenging missions in life science. Pluripotent stem cells derived brain organoids mimic the development, maturation, signal generation, and function of human brains, providing unique advantage for neurology. Single-cell RNA sequencing (scRNA-Seq) and multielectrode array independently revealed the similarity between brain organoids and immature human brain at early developmental stages, in the context of gene transcription and dynamic network of neuronal signals. Brain organoids provided the unique opportunity to investigate the underlying mechanism of neural differentiation, senescence, and pathogenesis. In this review, we summarized the latest knowledge and technology in the brain organoid field, the current and potential applications in disease models and pre-clinic studies, with emphasizing the importance of transcriptional and epigenetic analysis.

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Section: Epigenetic Modification Of Human Brain and Brain Organoidsmentioning

confidence: 99%

Genetic and Epigenetic Regulation of Brain Organoids

Wang

2022

Front. Cell Dev. Biol.

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“…Neurogenesis is a dynamic process associated with NSCs and NPCs differentiation into newborn neurons, which integrate into the local neural network in the mammalian CNS [39]. This process is a well-orchestrated sequence of…”

Section: The Function Of Lncrnas In Neurogenesis/neural Differentiationmentioning

confidence: 99%

The functions of long non-coding RNAs in neural stem cell proliferation and differentiation

et al. 2020

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The capacities for neural stem cells (NSCs) self-renewal with differentiation are need to be precisely regulated for ensuring brain development and homeostasis. Recently, increasing number of studies have highlighted that long non-coding RNAs (lncRNAs) are associated with NSC fate determination during brain development stages. LncRNAs are a class of non-coding RNAs more than 200 nucleotides without protein-coding potential and function as novel critical regulators in multiple biological processes. However, the correlation between lncRNAs and NSC fate decision still need to be explored in-depth. In this review, we will summarize the roles and molecular mechanisms of lncR-NAs focusing on NSCs self-renewal, neurogenesis and gliogenesis over the course of neural development, still more, dysregulation of lncRNAs in all stage of neural development have closely relationship with development disorders or glioma. In brief, lncRNAs may be explored as effective modulators in NSCs related neural development and novel biomarkers for diagnosis and prognosis of neurological disorders in the future.

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“…Under in vitro conditions, the differentiation of sufficient neurons from NSCs has been challenging. The extracellular matrix (ECM) and secreted molecules are the main players that affect cell behavior, including differentiation of stem cells into mature cells [ 11 , 12 , 13 , 14 ]. Therefore, research is currently in progress to determine if fabricated culture surfaces could help promote the differentiation of NSCs mostly into neurons.…”

Section: Introductionmentioning

confidence: 99%

Functionalized Nanocellulose Drives Neural Stem Cells toward Neuronal Differentiation

Pandanaboina

RanguMagar

Sharma

et al. 2021

JFB

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Transplantation of differentiated and fully functional neurons may be a better therapeutic option for the cure of neurodegenerative disorders and brain injuries than direct grafting of neural stem cells (NSCs) that are potentially tumorigenic. However, the differentiation of NSCs into a large population of neurons has been a challenge. Nanomaterials have been widely used as substrates to manipulate cell behavior due to their nano-size, excellent physicochemical properties, ease of synthesis, and versatility in surface functionalization. Nanomaterial-based scaffolds and synthetic polymers have been fabricated with topology resembling the micro-environment of the extracellular matrix. Nanocellulose materials are gaining attention because of their availability, biocompatibility, biodegradability and bioactivity, and affordable cost. We evaluated the role of nanocellulose with different linkage and surface features in promoting neuronal differentiation. Nanocellulose coupled with lysine molecules (CNC–Lys) provided positive charges that helped the cells to attach. Embryonic rat NSCs were differentiated on the CNC–Lys surface for up to three weeks. By the end of the three weeks of in vitro culture, 87% of the cells had attached to the CNC–Lys surface and more than half of the NSCs had differentiated into functional neurons, expressing endogenous glutamate, generating electrical activity and action potentials recorded by the multi-electrode array.

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Decoding epigenetic cell signaling in neuronal differentiation

Cited by 13 publications

References 209 publications

Genetic and Epigenetic Regulation of Brain Organoids

Genetic and Epigenetic Regulation of Brain Organoids

The functions of long non-coding RNAs in neural stem cell proliferation and differentiation

Functionalized Nanocellulose Drives Neural Stem Cells toward Neuronal Differentiation

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