Decoding the Complex Crossroad of Tryptophan Metabolic Pathways

Mondanelli, Giada; Volpi, Claudia; Orabona, Ciriana

doi:10.3390/ijms23020787

Cited by 8 publications

(5 citation statements)

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“…In the present study, the tryptophan metabolic pathway was significantly enriched in all three treatment groups. Tryptophan is an essential aromatic amino acid, and among the 20 common typical amino acids, tryptophan has the highest molecular weight ( 31 ). Although tryptophan is the least abundant amino acid in proteins and cells, it is a biosynthetic precursor for large number of microbial and host metabolites ( 32 , 33 ).…”

Section: Discussionmentioning

confidence: 99%

“…Compared with groups C50 and C65, xanthuronic acid was upregulated in group C80. The possible explanation for this phenomenon is that the increase of the concentrate ratio leads to increased generation of xanthurenic acid from tryptophan ( 31 , 34 ). In this way, subsequent reference numbers will be added by one digit, so that the sequence will not be disordered.…”

Section: Discussionmentioning

confidence: 99%

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Dietary Concentrate-to-Forage Ratio Affects Rumen Bacterial Community Composition and Metabolome of Yaks

Dai

et al. 2022

Front. Nutr.

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Changes in dietary composition affect the rumen microbiota in ruminants. However, information on the effects of dietary concentrate-to-forage ratio changes on yak rumen bacteria and metabolites is limited. This study characterized the effect of three different dietary concentrate-to-forage ratios (50:50, C50 group; 65:35, C65 group; 80:20, C80 group) on yak rumen fluid microbiota and metabolites using 16S rRNA gene sequencing and liquid chromatography-mass spectrometry (LC-MS) analyses. Rumen fermentation parameters and the abundance of rumen bacteria were affected by changes in the dietary concentrate-to-forage ratio, and there was a strong correlation between them. At the genus level, higher relative abundances of norank_f__F082, NK4A214_group, Lachnospiraceae_NK3A20_group, Acetitomaculum, and norank_f__norank_o__Clostridia_UCG-014 were observed with a high dietary concentrate-to-forage ratio (P < 0.05). Combined metabolomic and enrichment analyses showed that changes in the dietary concentrate-to-forage ratio significantly affected rumen metabolites related to amino acid metabolism, protein digestion and absorption, carbohydrate metabolism, lipid metabolism, and purine metabolism. Compared with the C50 group, 3-methylindole, pantothenic acid, D-pantothenic acid, and 20-hydroxy-leukotriene E4 were downregulated in the C65 group, while spermine and ribose 1-phosphate were upregulated. Compared to the C50 group, Xanthurenic acid, tyramine, ascorbic acid, D-glucuronic acid, 6-keto-prostaglandin F1a, lipoxin B4, and deoxyadenosine monophosphate were upregulated in the C80 group, while 3-methylindole and 20-hydroxy-leukotriene E4 were downregulated. All metabolites (Xanthurenic acid, L-Valine, N-Acetyl-L-glutamate 5-semialdehyde, N-Acetyl-L-glutamic acid, Tyramine, 6-Keto-prostaglandin F1a, Lipoxin B4, Xanthosine, Thymine, Deoxyinosine, and Uric acid) were upregulated in the C80 group compared with the C65 group. Correlation analysis of microorganisms and metabolites provided new insights into the function of rumen bacteria, as well as a theoretical basis for formulating more scientifically appropriate feeding strategies for yak.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dietary Concentrate-to-Forage Ratio Affects Rumen Bacterial Community Composition and Metabolome of Yaks

Dai

et al. 2022

Front. Nutr.

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“…Tryptophan is metabolized to form 5‐hydroxytryptophan which is used in the production of serotonin (Andreou et al., 2014). Therefore, oral consumption of CAE increases bioavailability of the monoamine neurotransmitters, dopamine, norepinephrine and serotonin (Mondanelli et al., 2022; Mondanelli & Volpi, 2021; Pero, 2010). This is particularly important because these neurotransmitters regulate attention.…”

Section: Discussionmentioning

confidence: 99%

Oral intake of carboxy alkyl ester improves attention: A randomized double‐blind cross‐over placebo‐controlled study

Guthrie,

Yang

2023

Human Psychopharmacology

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ObjectiveTo test the null hypothesis that oral intake of the dietary supplement carboxy alkyl ester (CAE) would have no effect on attention as revealed by mean rapid visual information processing (RVIP) scores.MethodsIn a randomized double‐blind cross‐over placebo‐controlled trial, healthy participants (age 19–66 years) of both sexes were randomly assigned to consume 700 mg of CAE or 700 mg of placebo. They received baseline attention testing via the RVIP task. Then they consumed CAE or placebo followed by RVIP testing. Participants were then given a washout period where they did not consume CAE or placebo. Afterward, individuals who initially consumed CAE were given the placebo and those who initially consumed the placebo were given CAE. Finally, all participants were tested again via RVIP.ResultsA priori statistical computation revealed that 30‐day oral intake of CAE improved mean RVIP test scores (t = 2.4, p < .05) relative to that at baseline, which resulted in a rejection of the null hypothesis.ConclusionsDaily oral intake of the CAE dietary supplement may boost attention and further research is now needed to confirm this observation.

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“…Tryptophan and its metabolites (kynurenine, NAD+, 5-HT, etc .) are essential for cell growth and cellular homeostasis ( Mondanelli, Volpi & Orabona, 2022 ; Platten et al, 2019 ). Another enriched pathway was the pentose phosphate pathway, which is a major pathway for glucose catabolism that produces NADPH and ribose 5-phosphate (R5P).…”

Section: Discussionmentioning

confidence: 99%

Metabolomics profiling reveals differences in proliferation between tumorigenic and non-tumorigenic Madin-Darby canine kidney (MDCK) cells

Sun,

Zhang,

Dong

et al. 2023

PeerJ

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Background Madin-Darby canine kidney (MDCK) cells are a cellular matrix in the production of influenza vaccines. The proliferation rate of MDCK cells is one of the critical factors that determine the vaccine production cycle. It is yet to be determined if there is a correlation between cell proliferation and alterations in metabolic levels. This study aimed to explore the metabolic differences between MDCK cells with varying proliferative capabilities through the use of both untargeted and targeted metabolomics. Methods To investigate the metabolic discrepancies between adherent cell groups (MDCK-M60 and MDCK-CL23) and suspension cell groups (MDCK-XF04 and MDCK-XF06), untargeted and targeted metabolomics were used. Utilizing RT-qPCR analysis, the mRNA expressions of key metabolites enzymes were identified. Results An untargeted metabolomics study demonstrated the presence of 81 metabolites between MDCK-M60 and MDCK-CL23 cells, which were mainly affected by six pathways. An analysis of MDCK-XF04 and MDCK-XF06 cells revealed a total of 113 potential metabolites, the majority of which were impacted by ten pathways. Targeted metabolomics revealed a decrease in the levels of choline, tryptophan, and tyrosine in MDCK-CL23 cells, which was in accordance with the results of untargeted metabolomics. Additionally, MDCK-XF06 cells experienced a decrease in 5’-methylthioadenosine and tryptophan, while S-adenosylhomocysteine, kynurenine, 11Z-eicosenoic acid, 3-phosphoglycerate, glucose 6-phosphate, and phosphoenolpyruvic acid concentrations were increased. The mRNA levels of MAT1A, MAT2B, IDO1, and IDO2 in the two cell groups were all increased, suggesting that S-adenosylmethionine and tryptophan may have a significant role in cell metabolism. Conclusions This research examines the effect of metabolite fluctuations on cell proliferation, thus offering a potential way to improve the rate of MDCK cell growth.

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Decoding the Complex Crossroad of Tryptophan Metabolic Pathways

Abstract: Among the 20 amino acids needed for protein synthesis, Tryptophan (Trp) is an aromatic amino acid fundamental not only for the synthesis of the major components of living cells (namely, the proteins), but also for the maintenance of cellular homeostasis [...]

Cited by 8 publications

References 16 publications

Dietary Concentrate-to-Forage Ratio Affects Rumen Bacterial Community Composition and Metabolome of Yaks

Dietary Concentrate-to-Forage Ratio Affects Rumen Bacterial Community Composition and Metabolome of Yaks

Oral intake of carboxy alkyl ester improves attention: A randomized double‐blind cross‐over placebo‐controlled study

Metabolomics profiling reveals differences in proliferation between tumorigenic and non-tumorigenic Madin-Darby canine kidney (MDCK) cells

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