Decoding the formation of diverse petal colors of Lagerstroemia indica by integrating the data from transcriptome and metabolome

Abstract

Lagerstroemia indica has great economic value due to its ecological, medicinal, and ornamental properties. Because its bloom color is one of the most essential characteristics, research into its color development is a hot topic. In this study, five representative colored cultivars were chosen, each representing a different color, such as white, red, pink, violet, and purple. Fully bloomed flowers were used to detect flavonoids in the petals. Anthocyanin is the main factor for the color formation of L. indica. 14 anthocyanins were discovered among the 299 flavonoids. Among 14 anthocyanins, malvidin-3,5-di-O-glucoside varied greatly among four colored samples and is the main contributor to color diversity. Transcriptome sequencing revealed that compared to white flowers, Anthocyanin pathway genes appear to be more active in colored samples. Analyzing the correlation network between metabolites and differential expressed genes, 53 key structural genes, and 24 TFs were detected that may play an essential role in the formation of color in L. indica flowers. Among these, the differential expression of F3′5′H and F3′H between all samples are contributors to color diversity. These findings lay the foundation for discovering the molecular mechanism of L. indica flower color diversity.

Multi-Omics Analysis Reveals That Anthocyanin Degradation and Phytohormone Changes Regulate Red Color Fading in Rapeseed (Brassica napus L.) Petals

“…Furthermore, delphinidin 3- O -glucoside, cyanidin 3- O -glucoside, petunidin 3- O -glucoside, and peonidin 3- O -glucoside also showed a decreasing trend [ 24 ]. Similar investigations were also reported [ 62 , 63 ]. Therefore, our metabolomics analysis once again proved the importance of anthocyanin content in petal coloration.…”

Integrated Metabolomic and Transcriptomic Profiles Provide Insights into the Mechanisms of Anthocyanin and Carotenoid Biosynthesis in Petals of Medicago sativa ssp. sativa and Medicago sativa ssp. falcata

“…Dihydromyricetin is a precursor in the synthesis of delphinidin, petunidin and malvidin. Previous research has suggested that delphinidin, petunidin and malvidin can endow plant tissues and organs with a purple color, and the gene, F3′5′H , is frequently associated with purple color formation [ 37 , 38 ]. In the present work, we detected that the gene, F3′5′H ( MsG0380013382.01 , Log 2 fold-change value = 1.80), is significantly upregulated in purple flowers compared to cream flowers.…”

Metabolomics and Transcriptomics Revealed a Comprehensive Understanding of the Biochemical and Genetic Mechanisms Underlying the Color Variations in Chrysanthemums

“…Metabolomic analysis is a post-genomic technology designed to provide a comprehensive profile of all metabolites (estimates at least 200,000) in a typical plant and represents an important branch of systems biology [ 39 ]. Via metabolomic analysis, many researchers have deeply studied the differences in flavonoid metabolites in crops, fruits, and flowers under different conditions to provide new insights into genetic breeding [ 40 , 41 , 42 , 43 , 44 ]. In this study, metabolomics technology was used to determine and analyze the metabolite content of different chrysanthemum cultivars.…”