Decreased activity of neutrophils in the presence of diferuloylmethane (curcumin) involves protein kinase C inhibition

Modern Pharmacological Approaches to Therapies: Substances Tested in Animal Models of Rheumatoid Arthritis

“…As resulted also from our previous experiments (Bauerova et al, 2005a, 2005b, 2008a, 2008bDrafi et al, 2010;Jancinova et al, 2009;Kogan et al, 2005;Macickova et al, 2010;Ponist et al, 2010;Sotnikova et al, 2009;Strosova et al, 2008Strosova et al, , 2009), all performed in the AA model, substances with antioxidant properties have a high potency to be used in therapy of RA. They decreased the progression of AA when administered to rats with AA over the period of 28 days.…”

Therapeutic actions of curcumin in bone disorders

“…This will lead to higher plasma levels, as well as higher intracellular levels of curcumin. Consequently, this enhancement leads to a significant antiproliferative effect (IC 50 ¼ 70 mM) in MDA-MB-231 cells as compared with free curcumin (IC 50 ¼ 90 mM at 72 h). 59 In consistence with that data, Wartlick et al 66 previously reported that the presence of albumin in nano-formulation increased the endocytic uptake and the accumulation of the loaded drug within tumor site.…”

“…49 Finally, it has been demonstrated that curcumin can reduce the formation of reactive species (oxygen and nitrogen ones) responsible for the degradation of the cartilage. 50 Recently, a randomized clinical trial strongly showed the efficiency of curcumin to reduce pain and improve physical outcome of patients. 51 Despite these positive results, further clinical studies should be designed to evaluate whether curcumin could prevent the development of OA or limit its progression.…”

The natural stilbenoid pinosylvin and activated neutrophils: effects on oxidative burst, protein kinase C, apoptosis and efficiency in adjuvant arthritis

“…The phosphorylation of protein kinase C (PKC) isoenzymes α and βII was detected as previously described [29] . Isolated human neutrophils (5×10 6 ) were incubated at 37 °C with pinosylvin for 1 min, stimulated with PMA (0.15 µmol/L, 1 min) and rapidly lysed by the addition of solubilization buffer (20 mmol/L Tris-HCl, 5 mmol/L EDTA, 1% Triton, 10% glycerol, 10 mmol/L sodium fluoride, 1 mmol/L sodium orthovanadate, 200 µmol/L PMSF (phenylmethylsulfonylfluoride), 2 µg/mL pepstatin, 2 µg/mL leupeptin, and 2 µg/mL aprotinin, pH 7.4).…”

“…Pinosylvin cytotoxicity was evaluated on the basis of ATP (adenosine triphosphate) liberation measured by the luciferinluciferase chemiluminescence method [29] . A suspension of isolated neutrophils (30 000/sample, 30 µL) and 20 µL of Tyrode's solution were incubated with 50 µL of pinosylvin (1-100 µmol/L) for 15 min at 37 °C.…”