Decreased expression of Dkk1 and Dkk3 in human clear cell renal cell carcinoma

Guo, Changcheng; Zhang, Xiaolong; Yang, Bin; Geng, Jiang; Peng, Bo; Zheng, Junhua

doi:10.3892/mmr.2014.2077

Cited by 29 publications

(22 citation statements)

References 38 publications

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“…Significant decrease in tumorigenic ability in nude mice was observed in SFRP1-silenced ccRCC cells [27]. The mRNA and protein expression level of DKK was significant decrease in ccRCC patients [28]. LZTFL1 regulates the nuclear translocation of β-catenin and correlates with Wnt signaling [29–31].…”

Section: Discussionmentioning

confidence: 99%

miR-106b-5p promotes renal cell carcinoma aggressiveness and stem-cell-like phenotype by activating Wnt/β-catenin signalling

et al. 2017

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PurposeTo examine the role of miR-106b-5p in regulating the cancer stem-cell-like phenotype in clear cell renal cell carcinomas (ccRCC).Experimental DesignReal-time PCR was performed to evaluate miR-106b-5p levels in ccRCC cell lines and patients specimens. A series of in vivo and in vitro assays were performed to confirm the effect of miR-106b-5p on ccRCC stemness phenotype.ResultsccRCC cells and tissues expressed more miR-106b-5p than normal controls. Gain- and loss-of-function studies demonstrated that overexpression of miR-106b-5p in ccRCC cells increased the spheres formation ability and the proportion of side population cells. Ectopic expression of miR-106b-5p in ccRCC cells increased tumour growth rates and the number of metastatic colonies in the lungs by using an orthotopic kidney cancer model and a tail vein injection model, respectively. Mechanistic studies revealed that, miR-106b-5p has an activating effect on Wnt/β-catenin signalling. miR-106p-5p overexpression simultaneously targets multiple negative regulators of the Wnt/β-catenin pathway, namely, LZTFL1, SFRP1 and DKK2. In addition, we also confirmed that miR-106b-5p and its targets expression correlates with the overall-survival of ccRCC patients from TCGA.ConclusionsThese findings suggest that miR-106b-5p mediates the constitutive activation of Wnt/β-catenin signalling, likely serving as a potential therapeutic target for ccRCC.

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Section: Discussionmentioning

confidence: 99%

miR-106b-5p promotes renal cell carcinoma aggressiveness and stem-cell-like phenotype by activating Wnt/β-catenin signalling

et al. 2017

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“…SATB2 has been shown to be highly expressed in osteosarcoma cells and has been used as a sensitive marker for this type of bone malignancy [Seong et al, ; Davis and Horvai, ;]. In contrast, some reports suggest that SATB2 acts as a tumor suppressor, however no patients with documented SATB2 haploinsufficiency have been reported to have malignancies thus far [Brocato and Costa, ; Guo et al, ; Mansour et al, ; Wu et al, ]. Thus, the precise role of SATB2 in cancer remains to be fully elucidated.…”

Section: Clinical Datamentioning

confidence: 99%

SATB2‐associated syndrome: Mechanisms, phenotype, and practical recommendations

Zárate

Fish

2016

American J of Med Genetics Pt A

123

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The SATB2‐associated syndrome is a recently described syndrome characterized by developmental delay/intellectual disability with absent or limited speech development, craniofacial abnormalities, behavioral problems, dysmorphic features, and palatal and dental abnormalities. Alterations of the SATB2 gene can result from a variety of different mechanisms that include contiguous deletions, intragenic deletions and duplications, translocations with secondary gene disruption, and point mutations. The multisystemic nature of this syndrome demands a multisystemic approach and we propose evaluation and management guidelines. The SATB2‐associated syndrome registry has now been started and that will allow gathering further clinical information and refining the provided surveillance recommendations. © 2016 The Authors. American Journal of Medical Genetics Part A Published by Wiley Periodicals, Inc.

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“…Loss of Dkk3 protein expression is associated with poor prognoses in patients with gastric cancer, indicating that it may be a biomarker for predicting lymph node involvement in these patients (35). Dkk3 has recently been implicated in clear cell renal cell carcinoma, and may present a novel molecular target for its diagnosis and treatment (36). Additionally, Dkk3 may represent a therapeutic target for the treatment of heart failure following myocardial infarction (37).…”

Section: Dkk3mentioning

confidence: 99%

Serum biomarker analysis in patients with recurrent spontaneous abortion

Guo

et al. 2017

Molecular Medicine Reports

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Recurrent spontaneous abortion (RSA) occurs in 1–5% of parturients. The sustained therapy and research for RSA is expensive, which is a serious issue faced by both patients and doctors. The aim of the present study was to detect protein expression profiles in the serum of RSA patients and healthy controls, and to identify potential biomarkers for this disease. A 1,000-protein microarray consisting of a combination of Human L-507 and L-493 was used. The microarray data revealed that eight serum protein expression levels were significantly upregulated and 143 proteins were downregulated in RSA patients compared with the healthy controls. ELISA individually validated 5 of these 151 proteins in a larger cohort of patients and control samples, demonstrating a significant decrease in insulin-like growth factor-binding protein-related protein 1 (IFGBP-rp1)/IGFBP-7, Dickkopf-related protein 3 (Dkk3), receptor for advanced glycation end products (RAGE) and angiopoietin-2 levels in patients with RSA. Sensitivity and specificity analyses were calculated by a receiver operating characteristics curve, and were revealed to be 0.881, 0.823, 0.79 and 0.814, with diagnostic cut-off points of 95.44 ng/ml for IFGBP-rp1, 32.84 ng/ml for Dkk3, 147.27 ng/ml for RAGE and 441.40 ng/ml for angiopoietin-2. The present study indicated that these four proteins were downregulated in RSA samples and may be useful as biomarkers for the prediction and diagnosis of RSA. Subsequent studies in larger-scale cohorts are required to further validate the diagnostic value of these markers.

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Decreased expression of Dkk1 and Dkk3 in human clear cell renal cell carcinoma

Cited by 29 publications

References 38 publications

miR-106b-5p promotes renal cell carcinoma aggressiveness and stem-cell-like phenotype by activating Wnt/β-catenin signalling

miR-106b-5p promotes renal cell carcinoma aggressiveness and stem-cell-like phenotype by activating Wnt/β-catenin signalling

SATB2‐associated syndrome: Mechanisms, phenotype, and practical recommendations

Serum biomarker analysis in patients with recurrent spontaneous abortion

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