Decreased Proliferation and Erythroid Differentiation of K562 Cells by siRNA-induced Depression of OCTN1 (SLC22A4) Transporter Gene

Abstract

It was suggested that OCTN1 is involved in the transport of physiological compounds that are important for cell proliferation and erythroid differentiation.

Functional Characterization of Ergothioneine Transport by Rat Organic Cation/Carnitine Transporter Octn1 (slc22a4)

“…[9][10][11] Furthermore, we previously suggested that OCTN1 is expressed preferentially in erythroid cells. 7,8) Accordingly, OCTN1 is considered to be a physiologically important transporter in several cells such as blood cells, whereas its physiological roles remain to be clarified. As substrates of OCTN1, organic cations such as TEA and carnitine were demonstrated considering from the closely related transporter family members such as organic cation transporters OCTs and carnitine transporter OCTN2.…”

“…creased apoptosis in K562 cells was associated with the suppressed expression of human OCTN1 with siRNA method. 8) Accordingly, we hypothesized that the OCTN1-mediated uptake of ergothioneine is important in protective function against oxidative damage and rat Octn1 may be involved in the uptake of the anti-oxidant by PC12 cells.…”

“…[3][4][5] Based on expression of OCTN1 in kidney, we previously found that OCTN1 was localized on the apical membrane of renal proximal tubular epithelial cells and suggested that it is involved in the tubular secretion of cationic compounds. 6) Furthermore, based on preferential expression of OCTN1 in erythroid cells, 7) we also suggested that its physiological role is closely associated with differentiation and/or growth of blood cells, 8) while its physiological substrates have not been clearly identified yet.…”

Functional expression of carnitine/organic cation transporter OCTN1 in mouse brain neurons: Possible involvement in neuronal differentiation

“…In peripheral organs, OCTN1 has been proposed to be involved in proliferation and differentiation of erythroid cells (Nakamura et al, 2007), and to be associated with 6 development of rheumatoid arthritis (Tokuhiro et al, 2003) and Crohn's disease .…”

Increased ergothioneine tissue concentrations in carriers of the Crohn's disease risk-associated 503F variant of the organic cation transporter OCTN1

“…Since ET is suggested to inhibit apoptosis,6 7 an increased tissue accumulation in 503F carriers may contribute to a defective innate immunity by prolonging inflammatory immune cell survival or by impairing removal of intracellular bacteria.…”